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Chaudhary Sarwan Kumar Himachal Pradesh Agriculture University, Palampur

Himachal Pradesh Krishi Vishvavidyalaya (renamed as Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya in June, 2001) was established on 1st November, 1978.The College of Agriculture (established in May, 1966) formed the nucleus of the new farm University. It is ICAR accredited and ISO 9001:2015 certified institution. The Indian Council of Agricultural Research has ranked this University at eleventh place among all farm universities of the country. The University has been given the mandate for making provision for imparting education in agriculture and other allied branches of learning, furthering the advancement of learning and prosecution of research and undertaking extension of such sciences, especially to the rural people of Himachal Pradesh. Over the years, this University has contributed significantly in transforming the farm scenario of Himachal Pradesh. It has developed human resources, varieties and technologies and transferred these to farming community enabling the State to receive the “Krishikarman award” of Govt. of India four times in row for food grain production among small states of the country. Today, the State has earned its name for hill agricultural diversification and the farming community has imposed its faith in the University.

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Subject: Agricultural Biochemistry × Author: Kumari, Nirupma × Start date: 2008 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    SSR markers based genetic diversity studies in Valeriana jatamansi Jones.
    (Palampur, 2021-10-28) Kumari, Nirupma; Sharma, Ram Kumar
    Valeriana jatamansi Jones. is a perennial, tetraploid (2n = 4x = 32), gynodioecious herb from family Valerianaceae. It is native to the Himalayan region, distributed from an altitude range of 1,000 to 3,000 m asl (above sea level) and in India, it is mainly distributed in the northern and north-eastern states. Due to great economic importance for its unique medicinal, flavour and aromatic properties the species have been subjected to great habitat degradation and fragmentation by the illegal trade. Additionally, plant also lacks any molecular genetics efforts mainly because of the non-availability of genome-wide molecular marker resources. In this study, a total of 9,988 SSR containing sequences were derived with maximum abundance of direpeats (~48%) followed by tri-repeats (~32%). Furthermore, synthesis and validation of 103 functionally SSR markers identified 51 polymorphic markers. Extrapolation of highly informative 10 SSR markers for genotyping 100 individuals representing ten diverse geographical populations suggests wide utilization of these markers in large scale fingerprinting studies of V. jatamansi germplasm. Neighbor- joining based hierarchical clustering and Bayesian structure clustering analysis corresponded each other and grouped 100 genotypes into two major clusters suggesting that there are two major genetic pools operating in Himachal Pradesh. Further, AMOVA analysis suggests maximum diversity exists within population (75%). Moderate genetic differentiation (Fst = 0.14) with significant gene flow (Nm = 1.79) may be attributed to natural bottlenecks and various anthropogenic activities in the region. Microsatellite markers identified in this study can be categorized as “Informative Markers” which can be further used for genotyping, genome mapping, evolutionary studies and implementation conservation plans for sustainable utilization of V. jatamansi.