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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Author: Pooja × End date: 2023 × Start date: 2023 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Computational studies of cytochrome P450 (CYPs) enzyme in pulses
    (CCSHAU, Hisar, 2023-10) Pooja; Ahalawat, Navjeet
    Cytochrome P450 (CYP450) is a large superfamily of enzymes found in all domains of life, including plants. It plays a crucial role in the metabolism of various compounds, including drugs, toxins, and endogenous substrates. Pulses are the richest source of fiber and complex carbohydrates, resulting in a low GI food. CYP450s enzymes are heme-containing monooxygenases that catalyze metabolisms of various endogenous and exogenous compounds. They constitute a superfamily of enzymes present in various organisms including mammals, plants, bacteria, and insects. CYP450s are diverse and metabolize a wide variety of substrates, but their structures are largely conserved. In this study, bioinformatics analyses of CYP450s enzyme performed in pulses. According to the conserved motifs obtained by MEME and MAST tools, ten motifs were common in all those pulses. The structural and functional analyses of CYP450s in pulses were done by using UniProtKB and InterProScan tools in ExPASy database. The tertiary structures of pulses were predicted by Phyre2 web server. The protein sequences were aligned with ClustalW and then the phylogenetic tree was constructed by MEGA11 using the neighbor-joining (NJ) method. By the successful analysis of CYP450s, we developed a productive database by using MYSQL and PHP. The database provides information related to 200 proteins of P450s in pulses with their predicted 3D structure in PDB format, functions and Fasta sequences. A Pulses Cytochrome P450 database serves as a centralized repository for curated information on CYP450 enzymes in pulses. It consolidates data from various sources, including sequence information, structural data, substrate specificity, and catalytic properties.
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    ThesisItemOpen Access
    Fabrication of bismuth telluride thin film and study of its electrical properties
    (CCSHAU, Hisar, 2023-05) Pooja; Paul Singh
    In this study, thin films of Bismuth Telluride were fabricated successfully by using thermal evaporation method onto different substrates (i.e., glass and silica) at ambient temperature. Their structural, morphological, optical and electrical properties were investigated by using X-ray diffraction (XRD), Field Emission-Scanning Electron Microscopy (FE-SEM), Photoluminescence (PL) sepectrooscopy and SourceMeter Instrument, respectively. The FT-IR study showed that the prepared Bismuth Telluride films do not show any transmittance spectra. The XRD analysis revealed that the films were crystalline in nature. The surface mimages showed that the prepared films have a homogeneous and compact grain surface. The calculated optical band gap was about 2 eV for both types of film. The I-V characteristics of prepared thin films were analyzed at temperature ranges from 30°C to 100°C. It was found that the films fabricated onto silica substrates showed larger electrical conductivity than that of the films fabricated onto glass substrates. Also, the increment in electrical conductivity was observed with the temperature showing that the prepared films have negative temperature coefficient of resistance and exhibit the semiconductor behaviour.
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    ThesisItemOpen Access
    Influence of manure and nano urea on productivity and quality of wheat (Triticum aestivum L.)
    (CCSHAU, Hisar, 2023-08-01) Pooja; Thakral, S.K.
    The present investigation entitled “Influence of manure and nano urea on productivity and quality of wheat (Triticum aestivum L.)” was carried out during Rabi 2022-23 at the Agronomy Research Farm, Department of Agronomy, CCS HAU, Hisar with the objectives to evaluate the effect of organic manures and nano urea on growth, quality, yield and economics of wheat. The research was carried out in factorial randomized block design with nine treatments with four replications. Factor A consisted of two wheat varieties (WH 1270 and DBW 303), assisted in main plot and while factor B comprised nine fertility levels, viz., 100% RDF, 125% RDF, 3 sprays of nano urea @ 500 ml/acre at 20-25, 40-45 and 60-65 DAS, 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS, 75% RDF + 2 sprays of nano urea @ 500 ml/acre at 20-25 and 40-45 DAS + PSB, 50% RDF + VC @ 4 t/ha + FYM @ 5t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS, 50% RDF + VC @ 4 t/ha + 2 sprays of nano urea @ 500ml/ acre at 20-25 and 40-45 DAS, 50% RDF + FYM @ 10 t/ha + Azotobacter @ 500 g/ha, 50% RDF + 2 sprays of nano urea @ 500 ml/acre at 20-25 and 40-45 DAS + Azotobacter @ 500g/ha + PSB in sub plots. Based on the present study it was be concluded that the variety WH 1270 recorded in significantly higher growth parameters such as plant height, dry matter accumulation, crop growth rate (CGR), relative growth rate (RGR) and yield attributing characters, viz., number of spikes per mrl-1 and 1000 grain weight as compared to the variety DBW 303. Higher grain yield (5154 kg ha-1) and straw yield (6099 kg ha-1) were registered by the variety WH 1270. Among the fertility levels, maximum plant height, crop growth rate, relative growth rate along with yield attributing like number of spikes per mrl-1, number of grains per spike and 1000- grain weight were noted by the treatment with application of 125% dose of fertilizer. This treatment resulted in dry matter accumulation, number of spikes per mrl-1,number of grains per spike and 1000- grain weight at par with the treatment with 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS. Maximum grains yield (5426 kg ha-1) was recorded with the treatment 125% RDF which was at par with the treatments 100% RDF (5350 kg ha-1) and 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS (5396 kg ha-1). Maximum straw yield (5422 kg ha-1) was observed with the treatment 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS were applied. Highest protein content (11.26%) was recorded with the treatment where 125% RDF was applied. It was 1.3% and 1.5% higher than the treatments with 75% RDF + FYM @ 5 t/ha + 1 spray of nano urea @ 500 ml/acre at 40-45 DAS (11.50) and 100% RDF (11.48). Highest net returns (₹ 86937 ha-1) and B: C (1.24) ratio were recorded with the treatment where 75% RDF + FYM at @ 5 t/ha with 1 spray of nano urea at 40-45 DAS was applied.