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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2010 - 20142
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Author: Aneja, Bharti × Start date: 2000 × Type: Thesis ×
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    ThesisItemOpen Access
    Transcript profiling of stress inducible MYB transcription factor and related genes in Indian mustard (Brassica juncea L. Czern. & Coss.)
    (CCSHAU, 2014) Aneja, Bharti; Yadav, Neelam R.
    Drought stress reduces the capacity of plants to take up water from soil, limiting plant growth and yield worldwide. The present investigation was carried out to study the expression of drought stress induced myb transcription factor genes (myb2, myb44, myb60) and related genes (hsp, pp2c and lhcb) in Brassica juncea cvs. RH0116 (drought tolerant) and RH8812 (drought sensitive) through semi-quantitative RT-PCR using actin gene as control. Brassica plants were raised in nethouse and subjected to drought stress by water withholding at flowering stage. We found that myb2, myb44, hsp and pp2c genes were upregulated and were expressed in both shoots and roots during drought stress. RH0116 showed higher accumulation of myb2, myb44 and pp2c transcripts as compared to RH8812. Whereas, myb60 and lhcb genes were downregulated during drought stress and their expression was limited only to shoots. The drought stress induced Atmyb44 homologues from RH8812 and RH0116 were cloned, sequenced and designated as BjMyb44-1 (872 bp) and BjMyb44-2 (862 bp) respectively. These had much similarity to other myb genes of Arabidopsis thaliana (84%) and Brassica rapa (94%). Multiple sequence alignment using CLUSTALW2 program with each other and with other sequences established homology. Seeds of both the genotypes were grown on MS medium with various levels of stress treatments using PEG 6000, Mannitol and ABA. Seed germination was arrested at initial stage only and reduction in seed germination was significant among treatments and no seedling growth was observed in case of ABA treatment. The average shoot and root lengths of seedlings decreased with the increasing dose of PEG & Mannitol in both the genotypes. The Brassica plants grown in vivo and two weeks old in vitro grown Brassica seedlings subjected to air drying stress treatments were used to assess the physiological parameters. The stressed plants showed lower Relative water content as compared to the control plants and the decline was higher in sensitive check. Osmotic potential was found to be more negative in case of stressed plants than control plants and decrease was relatively more in RH8812 as compared to RH0116. Electrolyte leakage increased in stressed plants as compared to control plants and RH8812 showed more increase than RH0116. Chlorophyll fluorescence study revealed that the value of ratio of variable fluorescence to minimum fluorescence (Fv/ Fm) was more in the control plants as compared to stressed plants, however, the increase was relatively more in RH8812 than RH0116.
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    ThesisItemOpen Access
    Micronutrient and molecular diversity analysis in mungbean [Vigna radiata (L.) wilczek] genotypes
    (CCSHAU, 2010) Aneja, Bharti; Yadav, Neelam R.
    Vigna radiata (L.) Wilczek, commonly called mungbean or greengram is the third most important pulse crop of India occupying nearly 3 million hectare. It is an excellent source of inexpensive and easily digestible proteins of low flatulence and is available in several edible forms. Iron and Zinc are important micronutrients for human health whose deficiency causes anemia and malfunctioning of immune system. Commercial cultivars contain low levels of iron and zinc and it is important to assess genetic variability in available germplasm for improving micronutrient content in commercial cultivars. The present study was undertaken to study SRAP polymorphism among 21 mungbean genotypes using 29 SRAP primers. A total of 121 amplified bands were produced which were polymorphic with an average of 4.65 bands per primer. The size of amplified bands ranged from 70-3000 bp. Unique bands were displayed by 8 mungbean genotypes using SRAP analysis. Six out of 29 SRAP primers were most useful in fingerprinting mungbean genotypes under study. The similarity coefficients between different genotypes ranged from 0.45-0.96 with an average similarity value of 0.71. At an arbitrary cut-off at 60 per cent similarity level on a dendrogram, the mungbean accessions were categorized into two major clusters. ML1108 and 2KM115 were found to genetically similar. SMH99-1A and ML776 showed high iron and zinc content while Satya was poor in iron as well as zinc content. Recombinant inbred lines involving ML776 and Satya could be used for tagging gene for micronutrient content. The results indicated that SRAP markers were efficient for identification of Vigna radiata genotypes and for determination of the genetic relationships among them.