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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Zoology × End date: 2018 × Type: Thesis ×
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    ThesisItemOpen Access
    Effect of heavy metals on bio-molecules and immune cells of earthworm, Eudrilus eugeniae
    (CCSHAU, 2018) Renu; Dharambir Singh
    Earthworms are important components of the soil ecosystem, because their burrowing and feeding activities contribute notably to increase water infiltration, soil aeration and the stabilization of soil aggregates. Recent years have witnessed the contamination of soil ecosystem by heavy metals, which are considered as one of the major environmental issue. Presence of heavy metals in the soil environment adversely affects the earthworm populations resulting in an imbalance of the normal functioning of the various components of the ecosystem and due to this reason; the present study was carried out to investigate the effect of heavy metals on biomolecules and immune cells of earthworm, E. eugeniae. The results obtained showed the acute toxicity of two heavy metals individually as well as in combination of different concentrations on earthworms in terms of survival rate, growth (length and weight), reproductive efficacy, cocoon production, bio-molecules and coelomocytes. Maximum reduction in length (4.47 cm) and weight (0.41 g) of earthworm was seen in case of soil treated with Pb-0.06ppm as compared to control. Similar decrease in the survivability of earthworms was 43.35% and number of cocoons reduced upto 24.67 in case of Pb-0.06ppm contaminated soil. The maximum reduction in bio-molecules i.e. 16.23% in crude protein 28.55% in carbohydrate and 44.74% in crude lipid concentration was seen in earthworms treated with Pb-0.06ppm. In case of coelomocytes, there was a significant reduction in the total number of coelomocytes (67.39 %) in worms exposed to Pb (0.06 ppm). Similarly significant reduction in eleocytes, amoebocytes-I, amoebocytes-II, granulocytes-I and granulocytes- II was seen. Thus the study proves that Lead is more toxic and detrimental to earthworms as compared to Nickel.
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    ThesisItemOpen Access
    Molecular characterization and management of Tyrophagus putrescentiae (Schrank) in button mushroom, Agaricus bisporus (Lange)
    (CCSHAU, 2018) Arvind; Gulati, Rachna
    Molecular characterization of acaricide susceptible, resistant and untreated Tyrophagus putrescentiae on mushroom clusters having a similarity coefficient of 0.45. Cluster one was represented by untreated (control) and resistant population sharing a similarity coefficient of 0.68 where as the cluster 2 was represented by susceptible population alone. Preliminary primers screening produced 22 polymorphic bands (70.96% polymorphism) and 9 monomorphic bands (29.03%). Methanolic seed and leaf extracts of P. pinnata showed acaricidal activity against T. putrescentiae. In Direct spray and Treated arena bioassay method, maximum mortality of 82, 76 % and 75.33, 63.33% was observed at 1.0 % concentration of seed and leaf extract, respectively. Both the extracts were equally effective against T. putrescentiae when direct spray on mites was done. The mortality was 59.63 and 58.15 percent in seed and leaf extract spray, respectively. Under Treated arena bioassay, seed extract was statistically more effective against T. putrescentiae (52.22 % mortality) as compared to leaf extract (43.56 % mortality). The LC50 value in Direct spray Treated arena bioassay method was 0.77, 0.63 and 0.85 and 0.64% in leaf and seed extract of P. pinnata. Among the solvents, hexane was most potent as 8, 5.66 and 0.33 mites were recovered from 0.5, 1 and 1.5 percent concentration after 36 h as compared to 38.66 mites in control. Bioefficacy studies conducted in compost bags under direct spray showed that among the treatments, Propargite (0.05%) significantly was more effective in reducing T. putrescentiae number (0.78 mites) than P. pinnata leaf extract at 0.752% (30 mites), 0.852% (18.89 mites) and 0.952% (12.22 mites) concentrations. P. pinnata seed extract at 0. 738% reduced the population to 5.88 mites which was at par with Propargite treatment.The role of T. putrescentiae as vector of dry bubble and green mould disease caused by fungus Verticillium fungicola and Trichoderma harzianum, respectively was also ascertained during the present study. V. fungicola reared T. putrescentiae population on Agaricus bisporus spread the disease within 8 days culture plates and fruiting bodies. Similarly, T. harzianum reared T. putrescentiae population on Agaricus bisporus spread the green mould disease to culture plates and fruiting bodies.A peak in population (47.40, and 60.80 mites) on V. fungicola and T. harzianum was recorded after 12 and 14 days, respectively which was statistically higher than the mites recorded at other observation periods.
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    ThesisItemOpen Access
    Comparative impact of insecticides on bacterial and acetylcholinesterase activity of Eisenia fetida (Savigny) and Pheretima posthuma (Kinberg)
    (CCSHAU, 2017) Yadav, Jyoti; Dharambir Singh
    The present study was carried out to analyze the toxicity of chlorpyrifos and carbofuran on E. fetida and P. posthuma. Paper contact toxicity test was used to determine LC50. LC50 of chlorpyrifos against adult E. fetida and P. posthuma was 0.25% and 0.11%, respectively. While LC50 of carbofuran against adult E. fetida and P. posthuma was 5.14% and 4.11%, respectively that confirms the greater sensitivity of P. posthuma towards insecticide induced toxicity in comparison to E. fetida. Lower LC50 value of chlorpyrifos confirms its higher toxicity as compared with carbofuran. Total 52 bacterial isolates were isolated from the gut of worms, out of which six resistant gut bacterial species having agricultural importance were identified as Sphingobacterium sp., Citrobacter freundii, Citrobacter murliniae, Bacillus australimaris and Burkholderia paludis. Inhibition in AChE activity due to chlorpyrifos and carbofuran exposure was observed at 24h. While E. fetida exposed to carbofuran tried to maintain AChE level, which may be seen as strategy to cope up with toxicity after prolonged exposure.
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    ThesisItemOpen Access
    Evaluation of management practices against Polyphagotarsonemus latus (Banks) and its role as vector of leaf curl virus in chilli
    (CCSHAU, 2018) Monika; Gulati, Rachna
    Under in vitro 35.00 to 67.27 and 33.89 to 63.89 percent mortality of P. latus was recorded in Direct Spray Bioassay, with 2.5 to 10.0 % and 1.25 to 10.0 % seed and leaf extract concentrations of P. pinnata, respectively. LC50 values of methanolic seed and leaf extract of P. pinnata were 5.36 and 5.31, respectively for mobile stages of P. latus. Under field conditions, the percent reduction in P. latus was from 40.60 to 72.55 percent with all the treatments concentration of P. pinnata. Under in vivo P. pinnata seed extract 10.0% (72.55%), was the most potent in reducing the mite population followed by P. pinnata leaf extract 10.0% (65.10 %), P. pinnata seed extract 7.5 % (62.66 %), P. pinnata seed extract 5.4 % (55.49%), P. pinnata leaf extract 7.5 % (54.98 %), P. pinnata seed extract 5.0 % (48.33%), P. pinnata leaf extract 5.3 % (47.58%), P. pinnata leaf extract 5.0 % (40.16%) and Pyriproxyfen 5% + Fenpropathrin 15% EC (1ml/l H2O) (37. 83%), respectively. Studies on seasonal incidence of P. latus revealed a peak in its population on chilli during second fortnight of October during the respective crop seasons in both the years under field conditions. Strata wise, top strata harboured significantly more number of mites followed by middle and bottom strata. A comparison of mulch treatments showed that Organic mulch 12t/ha was most effective in reduction of P. latus population (79.30 %), followed by Black/Silver polythene sheet (78.50 %), Black/White polythene sheet (78.30 %), Black polythene sheet (77.50 %), Organic mulch 9t/ha (74.70 %) and Organic mulch 6t/ha (65.30 %) treatment as compared to control. In terms of percent increase in yield, Organic mulch 12t/ha caused highest increase (57.90 % over control) followed by Black polythene sheet mulch, Black/Silver polythene sheet mulch, Black/White polythene sheet mulch, Organic mulch 9t/ha and Organic mulch 6t/ha showing 47.49, 46.72, 45.17, 42.47 and 34.36 percent increase over control. P. latus showed non-transmissible nature of virus. Thus it indicated that mite is not a carrier vector for transmission of leaf curl virus into chilli plant.
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    ThesisItemOpen Access
    Determining the relative efficacy of vermicompost, FYM and chemical fertilizers on crop yield of some vegetable crops
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Kumar, Dinesh; Sihag, R. C
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    ThesisItemOpen Access
    Studies on seasonal gonadal changes 1n common house rat, Rattus rattus Linnaeus
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Vir1nani, Shelly; Pasahan, S. C
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    ThesisItemOpen Access
    ldcntification, isolation and characterization or immunodominant antigens of adult Haemonchus contortus (Nematoda : Trichostrongylidae)
    (Department of College of Basic Sciences and Entomology Punjab Agricultural University Ludhiana, 2002) Kaur, Kanwaljeet; Sood, M.L
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    ThesisItemOpen Access
    Studies on the activities of insect-pollinators of cucurbit crops
    (College of Agriculture Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Kumar, Surender; Gahlawat, S. K
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    ThesisItemOpen Access
    Studies on the nematophagy in acarine fauna and their feeding potential on seed gall nematode
    (College of Basic Sciences and Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 2002) Rani, Ranjana; Gulati, Rachna