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Chaudhary Charan Singh Haryana Agricultural University, Hisar
Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.
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ThesisItem Open Access Studies on combining ability and economic heterosis in upland cotton (Gossypium hirsutum L.)(CCSHAU, 2011) Manish Kumar; Nirania, K.S.The present study was undertaken to estimate economic heterosis with the objective of exploring possibilities of its commercial utilization and to study the combining ability effects. The material for the present investigation comprised of 70 upland cotton genotype viz., 52 hybrids developed on 4 female parents (testers) using 13 male parents (lines) in line x testers mating design along with their 17 parents (4 females and 13 males) and one standard check (Hybrid HHH-223).The experimental material was grown at CCS Haryana Agricultural University, Cotton Research Station Sirsa, during Kharif 2010 in a randomized block design with three replications. Observations were recorded for nine characters namely, plant height, days to first flower, number of monopods, number of bolls, boll weight, ginning out turn, seed index, lint index and seed cotton yield per plant. The economic heterosis for all these characters was calculated over standard check HHH-223. Considerable amount of heterosis were recorded for seed cotton yield and other related characters under study. The hybrids H1226 x HS-1 and H1098 x DELTA SL exhibited heterosis of more than 65 per cent and hence warrant their further testing over locations for commercial utilization. The combining ability analysis revealed that both additive and non additive variances were present in the expression of all the characters with former playing major role for majority of the characters. In general, none of the male and female parents was found to posses high gca effects for all the characters under study. However, considering the economic importance of various characters DELCOT-517, F-1378, AUBURN NE-165, REX-66 and RS-875 among the males and H1226 among females may be used for future breeding programmes.ThesisItem Open Access In Vitro And In Vivo Selection Of Rice Genotypes For Salinity Tolerance And Their Characterization By Molecular Markers(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Bisht, Surinder Singh; Kishor, ChanderThesisItem Open Access Genetic Divergence, Stability Analysis And Characterization Of Citronella And Lemongrass (Cymbopogon Species)(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Singh, Ravinder; Verma, P.K.ThesisItem Open Access Genetic variability for zinc uptake and concomitant traits using agronomic and molecular indices in wheat (Triticum aestivum L.)(CCSHAU, 2013) Dinisha Abhishek; Sethi, S.K.A study was conducted to investigate variability for Zn, N, and P concentration in grain and straw, grain yield and its components, correlation and genetic diversity in wheat genotypes. The experiment laid out in completely randomized design included 15 hexaploid wheat cultivars, 12 F1 hybrids among them and 107 F2 plants. Results revealed that mean sum of squares due to genotypes (Parents and F1s) were significant for all the characters. Grain zinc concentration among parental lines ranged from 33.09 to 77.06 Ig/g, N concentration ranged from 2.79 to 5.10 % and P concentration ranged from 4171.00 to 5809.75 Ig/g. The highest level of Zn and N concentration was observed in cultivar C 306, and highest P concentration was observed in KRL 19. Some hybrids had more Zn, N and P concentrations while others had less as compared to their parental varieties The F2 population obtained from a cross between PBW 343 × KRL 19 showed large segregations for grain zinc (25.78 to 119 Ig/g ), N (1.5 to 5.2 %) and P (3292.35 to 8458.50 Ig/g) concentration. F1 hybrids had increased plant height, broader flag leaf area, more number of tillers and higher 1000-grain weight which resulted in higher grain yield when compared to their parental varieties. Correlation coefficients revealed no clear association of grain zinc concentration with grain yield and 1000-grain weight. However, phytic acid exhibited positive association with grain: zinc, protein and total phosphorus. Flag leaf area, 1000-grain weight, number of tillers and dry matter were significant component of grain yield. Genetic diversity for zinc assimilation in representative sets of wheat cultivars was studied at molecular level. Of 54 SSR primer pairs studied, 44 showed polymorphism, when screened in 15 wheat cultivars. A total of 165 alleles were identified averaging 3.1 alleles per locus. The value of average polymorphic information content for these SSR markers was estimated to be 0.42. The mean PIC value suggested that SSR marker system is effective in determining polymorphism. The SSR primer pairs found polymorphic in both PBW 343 and KRL 19 were selected to screen their 48 F2 plants. Of 20 polymorphic microsatellite markers screened, only 6 showed allelic variability both in parents and F2 plants with 30% polymorphism.ThesisItem Open Access Molecular Mapping Of Fusarium Wilt In Pigeonpea (Cajanus Cajan. L Millsp.)(Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Naresh Kumar; Singh, DhirajThesisItem Open Access Gene effects and characters association among the seed yield and it’s attributes in black gram (Vigna mungo L. hepper)(CCSHAU, 2010) Sukhwinder Singh; Lather, V.S.The present investigation was aimed to study gene effects of seed yield and other important characters and also study the characters associations among seed yield The genetic variation for yield and some important yield components was assessed in three sets of crosses involving four parents through generation mean analysis. The mean data of six populations (both parents, F1, F2, BC1 and BC2) were subjected to joint scaling test. In the presence of epistasis, six-parameter model was used to detect all types of gene effects. All three crosses had shown complex genetic behaviour for all the traits examined. The additive (d) and dominant (h) components of genetic variation were significant for all the traits in all the crosses except days to 50% flowering and days to maturity, but additive (d) component was non-significant for branches per plant in cross UH-04-06 X IPU-94-1 and UH-04-08 X IPU-94-1, for plant height in cross UH-04-08 X IPU-94-1. The duplicate type of non allelic interactions was found for all the characters except days to 50% flowering and days to maturity in all the crosses. The complementary type of non-allelic interaction for plant height was found in cross UH-04-04 X IPU-94-1. The F2 plants showed inbreeding depressions for almost all the characters in all the three crosses. A stable trend of positive correlation was observed among plant height, branches per plant, clusters per plant, pods per plant, seeds per pod, 100-seed weight and yield per plant.ThesisItem Open Access Genetic variation among disomic chromosome substitution lines of wheat variety C591 for grain yield attributes and nutrient uptake(CCSHAU, 2010) Surya, Aditya Kumar; Behl, R.K.disomic chromosome substitution lines of C591 in the background of Chinese Spring. The experiment was laid out in RBD with two treatments and replicated thrice in blocks under field condition. The data were recorded for different plant morphological characters, nutrient contents of all disomic lines and their parents and root characters of C591 and six DCS lines selected on the basis of grain weight Analysis of variance showed the presence of significant variability in the materials under study. Observations recorded on yield and its attributing traits and N, P, K and Zn contents showed genotype and chromosome specific variation. Correlation coefficients revealed highly significant to significant positive association among most characters. Highest magnitude of correlation coefficient was observed between P content (mg/plant) and grain yield per plant (g) and lowest for N content (mg/plant) and flag leaf area. On the basis of genotypic performance under various treatments for different plant characters, macro N, P, K and Zn uptake, DCS lines 2D, 4D, 1B performance were promising. Thus, It could be postulated that the quantitative traits loci for high N, P, K and Zn uptake were present on 2D, 3D chromosomes and for response on 6D. Data also showed that response genes and gene for performance are located on different chromosome. This indicated good scope for wheat improvement through analytical breeding at chromosome level.ThesisItem Open Access Genetic variability for grain quality attributes and HMW proteins in bread wheat, durum wheat and triticale(CCSHAU, 2010) Raosaheb, Magar Dhanaji; Chhabra, A.K.Genetic variability analysis is an important aspect of wheat and triticale grain quality improvement. The present study was conducted to analyze genetic variability in grain quality traits and HMW proteins. It was resolved into two experiments. The first experiment was conducted to assess the genotypic variation for grain quality traits of wheat and triticale genotypes. The observations on physico-chemical characteristics of wheat were recorded. These are directly or indirectly related to end-use quality, viz., grain weight (g), grain density (g/cc), grain crushing hardness (kg/seed), hectolitre weight (kg/hl), protein content (%), starch content (%), reducing and non-reducing sugars (%), total lipids (%), sedimentation value (ml), pelshenke value (min.), minerals [Cu, Fe, Mn, Zn (ppm)] and crude fibre (%). The data revealed significant genotypic variation for all the physico-chemical parameters of grain quality, viz., grain weight, grain density, grain crushing hardness, hectolitre weight, protein content, starch content, reducing and non-reducing sugars, total lipids, sedimentation value, crude fibre, pelshenke value, and all the minerals [Fe, Mn and Zn] except Cu content. The second experiment aimed at characterization of genotypes by protein profiling of seed storage proteins through electrophoresis (SDS-PAGE). The HMW subunit composition of 10 T.aestivum genotypes was determined and HMW glutenin subunits were identified and numbered. Overall quality scores of the HMW glutenin sub-units (Glu-1 quality scores) for a given genotype could thus be obtained as the sum of the scores were calculated. Allelic variations for HMW-glutenins were observed at each of the three complex loci i.e. Glu-1A, Glu- 1B and Glu-1D. The results revealed that the HMW glutenin subunit 5+10 was invariably associated with good bread making quality as inferred from grain quality score. The cluster analysis based on protein banding pattern broadly divided the wheat and triticale genotypes into two groups. Ten genotypes were differentiated from each other. Implication of results obtained in the study is discussed for wheat and triticale breeding.ThesisItem Open Access Genotypic diversity and stability analysis in chickpea (Cicer arietinum L.)(CCSHAU, 2010) Yadav, Asha; Yadav, C.K.Fifty genotypes of chickpea were evaluated in three different environments for the nature of variability and stability for eleven traits viz., days to 50% flowering, days to maturity, plant height , number of branches per plant, number of pods per plant, number of seeds per pod, biological yield, seed yield per plant, harvest index (%), 100 seed weight and protein content ( %). Analysis of variance revealed significant differences among the genotypes for all the characters studied. The highest GCV and PCV were observed for number of branches per plant followed by 100 seed weight, number of pods per plant, seed yield per plant, biological yield per plant. The highest heritability value was registered for protein content (%), plant height, days to 50% flowering, seed yield per plant, biological yield per plant. Genetic advance as % of mean was highest for 100 seed weight in E1 and E2 followed by seed yield per plant for number of pods per plant in E3 followed by number of branches per plant, harvest index and seed yield per plant. In the present study high heritability estimates accompanied with high genetic advance were observed for 100 seed weight in all the three environments. These genotypes were grouped into eight clusters each in first two environments (E1, E2) and into nine clusters in environment three (E3). On overall basis genotypes HC-3, HK-3, HK06-169, HK06-151, H05-10, H06-07, H06-32 and H07-23 were observed quite divergent in all the environments and could be used in hybridization programme for obtaining superior recombinants. Pooled analysis indicated wide differences between environments and differential behaviour of genotypes in different environments. Stability analysis showed that a major portion of genotype × environment (G × E) interaction was accompanied by linear component for days to 50% flowering, days to maturity, number of branches per plant, number of pods per plant, number of seeds per pod, biological yield, seed yield per plant, 100 seed weight, whereas, non-linear portion predominantly contributed towards plant height, number of branches per plant, harvest index and protein content (%). Environment two (E2) was observed to be best for most of the yield attributing traits. Based on stability parameters genotypes H06-79, H04-31, HK05-151, HK06-162, HK06-170, HK06-171, HK-2, HK-3, H06-32 were found stable in better environmental conditions, genotype HC-3 for poor environment and genotypes H05-10, HK06-152, HK06-155 across the environments.