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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Nematology × Author: Sharma, Lochan × Start date: 2020 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Studies on development and bioefficacy of chitosan based nano formulation (s) against root knot nematode, Meloidogyne incognita
    (Chaudhary Charan Singh Haryana Agricultural University hisar, 2022-11) Sharma, Lochan; Banakar, Prakash
    Present study was conducted to investigate the synthesis, characterization and bioefficacy of chitosan based nanoformulation (s) against root knot nematode, Meloidogyne incognita. Nanoparticles were synthesized by ionic gelation method and characterized by DLS, FTIR, FESEM and TEM. The DLS results confirmed the size, stability and charge of synthesized Cu-chitosan and chitosan-saponin NPs.Whereas FTIR, FESEM, FESEM-EDS and TEM, observations confirmed the functional group, crystalline porous structure, elemental compostion and spherical shape of synthesized nanoparticles. Encapsulation and release profile of Cu was confirmed by ICP-MS under varying pH and time.Maximum Cu release confirmed at pH 1 and 144 h of time period. In in vitro, assay results revealed that increasing the concentration and time exposure of Cu-chitosan and chitosan-saponin NPs leads to more nematode mortality and egg hatching inhibition. The maximum mortality and egg hatching inhibition was documented in 1000 ppm of Cu-chitosan NPs after 72 and 96 h respectively. In pluronic gel experiment NPs exposure to M. incognita juveniles for 16 h affect host finding behavior. A minimum number of juveniles reached around tomato roots were observed in chitosan-saponin NPs at 1200 ppm in 4 and 8 h as compared to water and octopamine control. Increasing the NPs concentration reduced nematode penetration and minimum number of nematode penetrated in 1200 ppm of chitosan-saponin NPs as compared water control in tomato roots. In management experiment foliar spray was more effective in increasing the plant growth parameters as compared to seedling root dip and soil drenching method. But, in reduction of nematode parameters observed in seedling root dip treatment (8 h) was more effective as compared to 4 h seedling root dip and other treatments. Cu-chitosan is more effective in reduction of number of galls, number of egg masses, eggs per egg mass and soil population per 200 cc soil, while chitosan-saponin NPs was more effective in increasing plant growth parameters. It was observed NPs also caused toxicity in M. incognita J2s and up regulates the detoxifying genes. Hence, the chitosan based nano formulation (s) proved to be of great nematicidal potential and opened new opportunities for expanding their effectiveness as new nematode control agents.