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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Molecular Biology and Biotechnology × Author: Sandhu, Nitika × End date: 2009 × Start date: 2009 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Development of F1 hybrids from crosses between aerobic and high-yielding rice cultivars and their identification via molecular marker analysis
    (CCSHAU, 2009) Sandhu, Nitika; Jain, R.K.
    Experiments were conducted to: (i) assess the plant type, yield potential and root traits of some of the aerobic rice genotypes (MAS25, MAS26, MAS109, 3508 and 3512) developed by UAS Bangalore and those selected at rice research station in comparison to the high-yielding indica (PAU201, HKR47) /basmati (HBC19, Pusa Sugandha 4 and Pusa Basmati 1460) rice varieties, (ii) make the crosses between aerobic and high-yielding indica/basmati rice varieties, (iii) assess the genetic diversity in a set of ten rice genotypes comprising of aerobic, indica and basmati rice varieties, and (iv) use microsatellite polymorphism for identification of F1 hybrids. Field evaluation showed that aerobic rice varieties produced optimum yield under aerobic conditions, which declined by 14-24% when cultivated under submerged conditions. On the contrary, indica/basmati had optimum yield under submerged conditions, which declined by 11-25% under aerobic conditions. Aerobic rice varieties have longer and dense root system compared to high-yielding indica/basmati rice varieties. Seedling length and fresh weight decreased under PEG-induced water stress, but the decreasing rate was lesser in aerobic rice varieties. A DNA fingerprinting database of ten parental aerobic, indica and basmati rice varieties have been prepared using 18 microsatellite and a BAD2 aroma gene specific markers. High level of polymorphism was observed among the aerobic, indica and Basmati rice varieties. Number of alleles per locus ranged from 3 to 5 with an average of 3.579 alleles per locus. Size of the PCR products amplified ranged from 80- 585 bp. The molecular size difference between the smallest and largest allele at a locus varied from 15 to 328. No Null alleles were detected. Multiple alleles were also detected at an average frequency of 0.263. Polymorphism information content (PIC), which is an indicative level of polymorphism, varied from 0.330 to 0.780 with an average of 0.620 per locus. The NTSYS-PC UPGMA tree cluster analysis showed the clustering of 10 rice genotypes into two major distinct groups. The group I had three Basmati rice varieties, HBC19, Pusa Sugandha 4 and Pusa Basmati 1460. The other group was further divided into two subgroups with subgroup 1 having indica rice varieties (HKR47 and PAU201) and other subgroup having all the aerobic rice varieties (MAS25, MAS26, MAS109, 3508 and 3512). Polymorphism for four (RM440, RM162, RM144, RM240) of the 18 SSR markers with a base difference of >30 bp in the amplified products, could be clearly visualized on 2.5% w/v agarose gels. A total of 22 crosses were made between aerobic (MAS25, MAS26, MAS109) and high yielding indica (HKR47, PAU201)/Basmati (HBC19, Pusa Sugandha 4 and Pusa Basmati 1460) varieties. The crossed seeds were recovered from 14 crosses. Two plants obtained from the Pusa Sugandha 4 x MAS25 and HBC19 x MAS26 crosses were confirmed as F1 hybrids by molecular marker analysis.