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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Molecular Biology and Biotechnology × Author: Mahavir × End date: 2009 × Start date: 2009 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Molecular characterization of cold tolerant and susceptible genotypes of chickpea (Cicer arietinum L.)
    (CCSHAU, 2009) Mahavir; Boora, Khazan Singh
    Chickpea (Cicer arietinum L.) is an annual grain legume cultivated in many parts of world and constitutes an important source of protein in human diet. Production of chickpea is affected by many biotic and abiotic factors. So, present investigation was undertaken to assess genetic differences between cold tolerant and susceptible genotypes of chickpea (Cicer arietinum L.) using SSR markers. Forty one SSR primers were used to assess molecular polymorphism in forty one chickpea genotypes. A total of 72 amplified products were obtained out of which 67 were polymorphic and 5 were monomorphic. Average polymorphism across forty genotypes was found to be 93.05%. For the genotypes tested, 1 to 7 bands were obtained, with an average of 1.75 bands per primer. The size of amplified fragments ranged from100-1300 bp. One primer also produced unique alleles in specific xx genotype E 100Ym which could be used to distinguish it. Analysis of this polymorphism profile, generated using suitable statistical programmes, grouped the forty one genotypes into two major clusters at a similarity coefficient of 0.60. These groups were further divided into sub groups and sub sub groups. The maximum similarity value of 0.94 obtained between Pusa 267 on the other hand PG 96006 & H04-11 and H04-11 & Pusa 244 found to be the most divergent with similarity value of 0.41. Genetic map was constructed and compared using Kosambi function. The marker regression function calculated by interval mapping the highest likelihood ratio statistic 3.668, 1.498, 1.429, 1.284, 0.848, 0.727, 0.693, 0.4 for each of the eight chromosomes at H1O09 (loc930), H2L102 (loc158), H1H24 (loc98), H1E06 (loc1), H1P17 (loc30), H4H05 (loc8), H1C19 (loc60) and H3H022 (loc21).These markers can be used in MAS for improvement of chickpea for cold tolerance. Fine mapping of the QTLs may be done to isolate the genes for cold tolerance using map based cloning.