Molecular characterization of cold tolerant and susceptible genotypes of chickpea (Cicer arietinum L.)
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Date
2009
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CCSHAU
Abstract
Chickpea (Cicer arietinum L.) is an annual grain legume cultivated in many parts of world and constitutes an important source of protein in human diet. Production of chickpea is affected by many biotic and abiotic factors. So, present investigation was undertaken to assess genetic differences between cold tolerant and susceptible genotypes of chickpea (Cicer arietinum L.) using SSR markers. Forty one SSR primers were used to assess molecular polymorphism in forty one chickpea genotypes. A total of 72 amplified products were obtained out of which 67 were polymorphic and 5 were monomorphic. Average polymorphism across forty genotypes was found to be 93.05%. For the genotypes tested, 1 to 7 bands were obtained, with an average of 1.75 bands per primer. The size of amplified fragments ranged from100-1300 bp. One primer also produced unique alleles in specific
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genotype E 100Ym which could be used to distinguish it. Analysis of this polymorphism profile, generated using suitable statistical programmes, grouped the forty one genotypes into two major clusters at a similarity coefficient of 0.60. These groups were further divided into sub groups and sub sub groups. The maximum similarity value of 0.94 obtained between Pusa 267 on the other hand PG 96006 & H04-11 and H04-11 & Pusa 244 found to be the most divergent with similarity value of 0.41. Genetic map was constructed and compared using Kosambi function. The marker regression function calculated by interval mapping the highest likelihood ratio statistic 3.668, 1.498, 1.429, 1.284, 0.848, 0.727, 0.693, 0.4 for each of the eight chromosomes at H1O09 (loc930), H2L102 (loc158), H1H24 (loc98), H1E06 (loc1), H1P17 (loc30), H4H05 (loc8), H1C19 (loc60) and H3H022 (loc21).These markers can be used in MAS for improvement of chickpea for cold tolerance. Fine mapping of the QTLs may be done to isolate the genes for cold tolerance using map based cloning.
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Keywords
Chickpeas, Genotypes, Dna, Genetics, Planting, Polymorphism, Tolerance, Genetic structures, Crops, Genomes