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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2009 - 200912010 - 20131
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Subject: Microbiology × Author: Chaudhary, Deepika × Start date: 1998 × Type: Thesis ×
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    ThesisItemOpen Access
    Amelioration of salt stress in chickpea (Cicer arietinum L.) by inoculation of ACC deaminase-containing mesorhizobia and rhizobacteria
    (CCSHAU, 2013) Chaudhary, Deepika; Sindhu, S.S.
    Chickpea is a major legume crop grown in the semi-arid tropics of Asia and Africa. Salinity mainly affects plant growth by decreasing the availability of water to the roots due to osmotic effect of external salts. Salinity also influences other physiological processes such as seed germination, photosynthesis, respiration and metabolite accumulation. The use of plant growth-promoting rhizobacterial as inoculants have been reported to facilitate plant growth in saline lands. Fifty isolates of Mesorhizobium were obtained from the nodules of chickpea plant samples and fifty five isolates of rhizobacteria were obtained from the chickpea rhizosphere soil collected from different part of Haryana. Among the fifty Mesorhizobium isolates, only four isolates i.e., MHD2, MSD41, MHD12 and MHD14 showed growth up to 4% NaCl salt concentration whereas 28 isolates among the 55 rhizobacterial isolates showed growth up to 4% salt with different colony size. Two Mesorhizobium isolates i.e., MHD1 and MHD12 and six rhizobacterial isolates i.e., RSD17, RSD19, RSD23, RBD12, RHD2 and RHD18 showed significant growth on ACC supplemented plates as compared to ammonium sulphate incorporated medium plates. Inoculation of selected Mesorhizobium or rhizobacterial isolates on sterilized chickpea seeds in water agar plates showed that isolate KR48 showed maximum growth of seedling roots at 5 days whereas MBD26 showed maximum growth of shoot at 10 days. Isolates RHD18, RSD3, RBD12 and RSD23 showed significant stimulation of shoot growth even at 8 dS/m salt concentration as compared to uninoculated control. Coinoculation studies with ACC+ as well as ACC- isolates of Mesorhizobium and rhizobacterial isolates were made in chickpea cultivar HC-1 under chillum jar conditions containing sloger’s broth with salt (EC, 4dS/m) and without salt. Coinoculation of ACC+ Mesorhizobium isolate MBD26 with rhizobacterial isolate RHD18 produced maximum 59 nodules/plant and 50.6% increase in plant dry weight and in the presence of salt slight decrease in nodulation (49 nodules/plant) and gain in plant dry weight (39.3%) was observed in comparison to single inoculation of MBD26 at 50 days of plant growth. At 80 days of plant growth, coinoculation of both ACC- MBD20 and RBD19 formed 41 nodules/plant and only 7.8% increase in shoot dry weight was observed as compared to the single inoculation. Coinoculation of Mesorhizobium isolate MBD26 with rhizobacterial isolate RHD18 caused increase in nodule number (78 nodules/plant), nodule weight (413 mg/plant) and shoot dry weight (61.7% increase). Whereas in the presence of salt, same treatment formed 53 nodules per plant and caused only 53.2% increase in plant biomass in comparison to single Mesorhizobium inoculated plants at 80 days of plant growth.
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    ThesisItemOpen Access
    Isolation and characterization of salinity tolerant azotobacter spp. (free living diazotrophs) for wheat crop (T. aestivum L.)
    (CCSHAU, 2009) Chaudhary, Deepika; Narula, Neeru
    Saline soil is a major agricultural problem and significantly affects plants and the microbial activity. Development of a biofertilizer for crops those grown under saline soil conditions are very important and are of utmost importance of under present day crisis. For different crops no biofertilizers are available in saline soil conditions. Keeping in mind the present investigation deals with isolation and characterization of salinity tolerant Azotobacter spp. (free living diazotrophs) for wheat crop. Different soil samples have different electrical conductivity those used during the study. Different morphological, biochemical and beneficial properties like IAA, ammonia excretion and fixed nitrogen were studied in all the isolates. On the basis of these properties 5 soil isolates (ST3, ST6, ST9, ST17 and ST24) were selected and used as inoculant for wheat (var. WH-157) in saline soil under pot house conditions. Maximum increase in plant parameters viz. plant height, seed yield, dry weight and % nitrogen were observed in ST24 soil isolate. Isolate ST24 showed plant height(cm) of 68.4, 78.4 and 89.9, seed yield of (g) 4.8, 5.0 and 6.1 , shoot dry weight of (g) 5.9, 7.4 , 12 and 0.40, 0.58, 0.70 percent nitrogen as compared to their respective controls. Bacterial survival of ST24 was maximum at 30 DAS 7.95 ×104, 8.23 ×104, 8.50 ×104 cfu ml-1 and decrease at 60 DAS and 90 DAS compared to their respective controls.