Amelioration of salt stress in chickpea (Cicer arietinum L.) by inoculation of ACC deaminase-containing mesorhizobia and rhizobacteria
Loading...
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
Chickpea is a major legume crop grown in the semi-arid tropics of Asia and Africa. Salinity
mainly affects plant growth by decreasing the availability of water to the roots due to osmotic effect of
external salts. Salinity also influences other physiological processes such as seed germination,
photosynthesis, respiration and metabolite accumulation. The use of plant growth-promoting
rhizobacterial as inoculants have been reported to facilitate plant growth in saline lands. Fifty isolates
of Mesorhizobium were obtained from the nodules of chickpea plant samples and fifty five isolates of
rhizobacteria were obtained from the chickpea rhizosphere soil collected from different part of
Haryana. Among the fifty Mesorhizobium isolates, only four isolates i.e., MHD2, MSD41, MHD12 and
MHD14 showed growth up to 4% NaCl salt concentration whereas 28 isolates among the 55
rhizobacterial isolates showed growth up to 4% salt with different colony size. Two Mesorhizobium
isolates i.e., MHD1 and MHD12 and six rhizobacterial isolates i.e., RSD17, RSD19, RSD23, RBD12,
RHD2 and RHD18 showed significant growth on ACC supplemented plates as compared to
ammonium sulphate incorporated medium plates. Inoculation of selected Mesorhizobium or
rhizobacterial isolates on sterilized chickpea seeds in water agar plates showed that isolate KR48
showed maximum growth of seedling roots at 5 days whereas MBD26 showed maximum growth of
shoot at 10 days. Isolates RHD18, RSD3, RBD12 and RSD23 showed significant stimulation of shoot
growth even at 8 dS/m salt concentration as compared to uninoculated control. Coinoculation studies
with ACC+ as well as ACC- isolates of Mesorhizobium and rhizobacterial isolates were made in
chickpea cultivar HC-1 under chillum jar conditions containing sloger’s broth with salt (EC, 4dS/m)
and without salt. Coinoculation of ACC+ Mesorhizobium isolate MBD26 with rhizobacterial isolate
RHD18 produced maximum 59 nodules/plant and 50.6% increase in plant dry weight and in the
presence of salt slight decrease in nodulation (49 nodules/plant) and gain in plant dry weight (39.3%)
was observed in comparison to single inoculation of MBD26 at 50 days of plant growth. At 80 days of
plant growth, coinoculation of both ACC- MBD20 and RBD19 formed 41 nodules/plant and only 7.8%
increase in shoot dry weight was observed as compared to the single inoculation. Coinoculation of
Mesorhizobium isolate MBD26 with rhizobacterial isolate RHD18 caused increase in nodule number
(78 nodules/plant), nodule weight (413 mg/plant) and shoot dry weight (61.7% increase). Whereas in
the presence of salt, same treatment formed 53 nodules per plant and caused only 53.2% increase in
plant biomass in comparison to single Mesorhizobium inoculated plants at 80 days of plant growth.
Description
Keywords
Planting, Biological development, Root nodulation, Bacteria, Application methods, Food preservation, Vegetative propagation, Chickpeas, Ethylene, Tolerance