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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Genetics × End date: 2008 × Start date: 2008 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Evaluation of recombinant inbred lines (RILs) of bread wheat for thermo response at seedling stage
    (CCSHAU, 2008) Sharma, Vikas; Yadava, Ramesh Kumar
    The present study has been conducted to Evaluation of recombinant inbred lines (RILs) of bread wheat for thermo response at seedling stage. The seed weight (g), seed density (mg ml-3), shoot length (cm), root length (cm), seedling length, fresh weight (mg), dry weight (mg), vigour (I), vigour (II) and germination % in 100-RILs of Harrier x S308 wheat population were observed at three temperature conditions (15º, 20º and 25ºC) with two replications. The estimates of mean, components of genetic parameters (GCV, PCV,h2, genetic advance etc.) and correlation coefficients along with divergence analysis has been worked out after the establishment of substantial amount of variability in the present material. The estimates of h2 (BS) were high for almost for all traits. Genetic advance expressed as % of mean revealed the maximum scope of improvement is for shoot length at 15ºC. Correlation coefficient was indicated to be highest for dry weight and fresh weight at all the three temperatures, and was followed by seedling length with shoot length and that with vigour I at 15ºC; fresh weight with vigour II at 20º and 25ºC and that of dry weight with vigour I at 20ºC and with vigour II at 25ºC. Euclidean Clustering revealed that these RILs could be grouped in 9 clusters at 15ºC, where as in 8 clusters at 20ºC and 25ºC. Only one combination of RILs HS-135 & -136 had the tendency of grouping under same cluster across all the temperatures. Cluster II had maximum xii number of genotypes (24) at 15ºC whereas cluster III at 20ºC, and 25ºC had 20 and 19 genotypes respectively. The cluster VIII had the minimum number of RILs (two) at 15ºC where as four at both 20ºC and 25ºC in each case. At 15ºC RILs in cluster I were better for SL, RL, Se L, VI in cluster III were better for FW, GP, and that of cluster VIII were better for SW, SD, DW, VII as revealed by cluster means, RILs grouped in cluster I at 20ºC were better for RL, Se L, FW, DW, VI, VII; and that of cluster II were better for SW, SL, whereas in case of cluster III were better for SD, GP on the basis of cluster means. Likewise, RILs in cluster I were better for SW, SD; of cluster II being better for FW, DW, VII, GP, and that of cluster III as better for SL, RL, Se L, VI , as indicated by cluster means at 25oC. Consistently higher mean values at all the three temperatures has been recorded in HS 104 for fresh weight, HS 3 for vigour I & HS 51 for germination %, whereas the RIL HS 98 had consistently lower means in case of fresh weight, dry weight and vigour II, HS 99 for shoot length & HS 86 for dry weight at all the three temperatures. RILs specific for improving the traits, shoot length, root length, seedling length, fresh weight and the various seedling vigour parameters of wheat under diverse temperature conditions have been identified.
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    ThesisItemOpen Access
    Genetic and symbiotic characterization of poly-β-hydroxybutyrate production in Rhizobium and Bradyrhizobium spp. nodulating mungbean [Vigna radiata (L.) Wilczek]
    (CCSHAU, 2008) Sandeep Kumar; Yadav, A. S.
    Twenty-one WT strains of Rhizobium/Bradyrhizobium sp. (Vigna) were isolated purified and tested for their ability to form nodules on the mungbean plants. These WT strains were screened for PHB production on Beringer’s MM with Nile blue A under UV light. The two WT strains MBR 16 and MBR 25 produced high amount of PHB and these were used to isolate PHB mutants. Three types of mutants were isolated and characterized as MHt, MM and ML mutants. The MHt mutants produced highest amount of PHB, whereas ML mutants produced very low amount of PHB. The Maximum amount of PHB was found in MBR 16 MHt 1 i. e. 1.24 g/l. The antibiotic resistance pattern of parent strains and their mutants was almost same. The symbiotic effectivity of parent strains and their mutants on mugbean plants showed that the MHt mutants had higher shoot dry weight and total shoot nitrogen than the MM and ML mutants. A highly significant positive correlation was found between the amount of PHB produced and shoot dry weight and total shoot nitrogen of the munbean plants.
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    ThesisItemOpen Access
    Studies on wheat-azotobacter interaction under pot house conditions
    (CCSHAU, 2008) Kusum; Vasudava, Manjula
    To study the varietal behaviour of wheat with regard to different strains of Azotobacter chroococcum, as experiment was conducted in which six varieties of wheat such as C-306, PBW-343, WH-283, WH-542, WH-147 and WH-711 were treated with two mutants of A. chroococcum Mac27 and HT54 at three levels of fertilizer such as O kg N ha-1, 90 kg N ha-1, 120 kg N ha-1 in terms of agronomical parameters under pot house conditions. Response of wheat varieties was studied towards A. chroococum in plant height, seed yield, total nitrogen content and total biomass. Variety C-306 showed best response towards both the strains of A. chroococcum. Mac27 showed better response as compared to HT54. Root exudates of these wheat varieties were analyzed for chemotactic behaviour and other components such as sugars, amino acids, phytohormones and organic acids. Glutamic acid and some other unidentified amino acids were detected in both inoculated and uninoculated wheat varieties. Succinic acid was present in wheat varieties WH-711 and WH-147. Maleic acid and some unidentified organic acids were detected in inoculated and uninoculated wheat varieties C-306, PBW-343, WH-283 and WH-542.
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    ThesisItemOpen Access
    Rhizobacterial strains as biocontrol agents against various soil pathogens
    (CCSHAU, 2008) Chauhan, Sapna; Vasudeva, Manjula
    A total of fifty three isolates/mutant were screened for their antagonistic potential against three selected fungal soil pathogens; Rhizoctonia solani cotton, R. solani rice and Fusarium oxysporum. Out of these 10, 6 and 12 isolates/mutant of Azotobacter chroococcum were selected against R. solani cotton, R. solani rice and Fusaraium oxysporum tomato respectively. 19.61% showed zone of inhibition against R. solani cotton, 11.76% against R. solani rice and 23.53% against Fusarium oxysporum tomato respectively. Further quantitative studies were made and these zones of inhibition were measured. In R. solani cotton, the zone was maximum (2276.5 mm2) with AH2 and minimum (141.3 mm2) with AM1. Similarly, in R. solani rice, the zone of inhibition was maximum (1243.44 mm2) with AVK42 and minimum (120.5 mm2) with AR4 and in Fusarium oxysporum, maximum (1004.8 mm2) with HT54 ii and minimum (164.05 mm2) with 103. Further, various studies were done to isolate, evaluate and characterize the antimicrobial substance responsible for this activity. The antimicrobial substance responsible for the antagonistic activity was found to be extracellularly produced and it was not thermostable. TLC assays were done to confirm the production of antifungal and antibiotic compounds taking Pseudomonas as standard. The invitro results were further checked in pothouse conditions on three crops, cotton, guar and tomato respectively. Increase in percent germination was observed. Maximum percentage of disease index (80.36 % and 93.75% on 60th day) was observed with R. solani cotton and R. solani rice check respectively while minimum disease index was seen in case of 103DS (16.67 %) in R. solani cotton and (2.5%) with 103DB in R. solani rice in cotton crop. Similarly in guar crop, maximum percentage of disease index (95.24% and 85.93% on 60th day) was observed with R. solani cotton and R. solani rice check respectively while minimum disease index was seen in case of AH2 (2.5%) in R. solani cotton and (13.29%) with AR4 in R. solani rice. Further in case of tomato, maximum disease index (85 % on 60th day) was observed in control Fusarium oxysporum check and was negligible in strains HT57, AP4, AVK42, AVK51, 103, AH2 and 103DS. The disease was completely controlled by these selected isolates/mutants.