Evaluation of recombinant inbred lines (RILs) of bread wheat for thermo response at seedling stage
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Date
2008
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Publisher
CCSHAU
Abstract
The present study has been conducted to Evaluation of recombinant inbred lines (RILs)
of bread wheat for thermo response at seedling stage. The seed weight (g), seed density (mg
ml-3), shoot length (cm), root length (cm), seedling length, fresh weight (mg), dry weight (mg),
vigour (I), vigour (II) and germination % in 100-RILs of Harrier x S308 wheat population were
observed at three temperature conditions (15º, 20º and 25ºC) with two replications. The estimates
of mean, components of genetic parameters (GCV, PCV,h2, genetic advance etc.) and correlation
coefficients along with divergence analysis has been worked out after the establishment of
substantial amount of variability in the present material.
The estimates of h2 (BS) were high for almost for all traits. Genetic advance expressed as
% of mean revealed the maximum scope of improvement is for shoot length at 15ºC. Correlation
coefficient was indicated to be highest for dry weight and fresh weight at all the three
temperatures, and was followed by seedling length with shoot length and that with vigour I at
15ºC; fresh weight with vigour II at 20º and 25ºC and that of dry weight with vigour I at 20ºC and
with vigour II at 25ºC.
Euclidean Clustering revealed that these RILs could be grouped in 9 clusters at 15ºC,
where as in 8 clusters at 20ºC and 25ºC. Only one combination of RILs HS-135 & -136 had the
tendency of grouping under same cluster across all the temperatures. Cluster II had maximum
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number of genotypes (24) at 15ºC whereas cluster III at 20ºC, and 25ºC had 20 and 19 genotypes
respectively. The cluster VIII had the minimum number of RILs (two) at 15ºC where as four at
both 20ºC and 25ºC in each case.
At 15ºC RILs in cluster I were better for SL, RL, Se L, VI in cluster III were better for
FW, GP, and that of cluster VIII were better for SW, SD, DW, VII as revealed by cluster means,
RILs grouped in cluster I at 20ºC were better for RL, Se L, FW, DW, VI, VII; and that of cluster
II were better for SW, SL, whereas in case of cluster III were better for SD, GP on the basis of
cluster means. Likewise, RILs in cluster I were better for SW, SD; of cluster II being better for
FW, DW, VII, GP, and that of cluster III as better for SL, RL, Se L, VI , as indicated by cluster
means at 25oC.
Consistently higher mean values at all the three temperatures has been recorded in HS
104 for fresh weight, HS 3 for vigour I & HS 51 for germination %, whereas the RIL HS 98 had
consistently lower means in case of fresh weight, dry weight and vigour II, HS 99 for shoot
length & HS 86 for dry weight at all the three temperatures. RILs specific for improving the
traits, shoot length, root length, seedling length, fresh weight and the various seedling vigour
parameters of wheat under diverse temperature conditions have been identified.
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Keywords
Sowing, Segregation, Developmental stages, Diseases, Crossing over, Genes, Seed treatment, Biological phenomena, Plant habit, Crops