Thumbnail Image

Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

Browse

2010 - 20142
Reset filters
Subject: Genetics × Author: Gupta, Mamta × End date: 2014 × Start date: 2010 × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    Genetic analysis of terminal heat tolerance in wheat using molecular markers
    (CCSHAU, 2014) Gupta, Mamta; Chawla, Veena
    The present investigation was carried out for genotyping and phenotyping of F2 population for heat tolerance in wheat and identification of putative QTLs using F2 population of cross PBW373 x WH1081 and PBW373 x PBW343. Three parents and four check varieties (Raj3765, WH730, DBW17 and WH711) were evaluated in field under normal sown (29.Nov.2011)and late sown (3.Jan.2012) conditions. Two factor ANOVA exhibited highly significant differences for genotypes, environments, as well as for genotype x environment interactions for days to heading, days to anthesis, days to physiological maturity, grain filling duration, plant height, number of productive tillers/plant, number of grains/spike, 1000 grain weight, grain yield/plant, biomass/plant, harvest index and membrane thermostability. HSI (Heat susceptibility index) for 1000 grain weight and grain yield/plant was observed to be significantly low in all tolerant genotypes (PBW373, Raj3765 and WH730). The performance of sensitive parents was affected to more extent in late sown conditions as compared to that in tolerant parents. Grain yield/plant showed a positive correlation with plant height (0.69**, 0.28**), biomass/plant (0.98**, 0.94**) and number of productive tillers/plant (0.89**, 0.85**) in both the crosses. For genotyping DNA from three parents PBW373, WH1081 and PBW343 was amplified using 200 SSRs. 158 SSRs showed amplification in parents. Only 22 SSRs produced polymorphic bands, of size between 100 to 300 bp and an average of 1.45 alleles. No polymorphism was observed in parents of other population. SSR amplification profile of 152 F2plants was used for identification of QTLs using QTL Cartographer version- 2.5. The single marker analysis identified 19 markers indicating the putative QTLs for yield, its components and heat stress related physiological traits. A total 22 SSRs were used to construct the map and these were found to be mapped on 16 linkage group. The number of markers on these 16 linkage groups varied from one to four. On A genome 13 QTLs were identified, on B genome 5 QTLs and on D genome 9 QTLs recepectively. The A-, Band D- genomes had 1360.3 cM, 272.4 cM and 919.5 cM of linkage coverage with average interval distances of 104.63 cM, 54.48 cM and 102.16 cM/Marker. A total of nine QTLs were resolved following composite interval mapping,one QTL was detected at a LOD score equal to threshold value of 2.5 while eight at LOD scores above the threshold value. All the nine QTLs were shown to be definitive located on chromosome 3A (QDh.CCSHAU-3A, QDa.CCSHAU-3A and QPm.CCSHAU-3A), cromosome 5A (QBm.CCSHAU-5A, QCtd.CCSHAU-5A and QCl.fl.CCSHAU-5A), chromosome 6A (QPh.CCSHAU-6A) and chromosome 3B (QTgw.CCSHAU and QMts.CCSHAU-3B). Positive QTL effect suggested that an allele of the above QTL for heat stress tolerance is available in the tolerant parental genotype PBW373. These putative QTLs indicated by the SSRs may be used in MAS after further validation using another mapping population.
  • Thumbnail Image
    ThesisItemOpen Access
    Molecular analyses of recombinant inbred lines of wheat cross aldan x WH542 for resistance to Karnal bunt (Neovossia indica)
    (CCSHAU, 2010) Gupta, Mamta; Chawla, Veena
    Present studies were conducted on two genotypes, Aldan (resistant) and WH542 (susceptible), and their 94 recombinant inbred lines. Investigations were carried out to screen the recombinant inbred lines (RILs) for Karnal bunt resistance and to characterize above lines using molecular markers. There was significant variation among all the recombinant inbred lines for coefficient of infection of Karnal bunt. The range of coefficient of infection (CI) on the RILs was 0-41.70. The chi-square analysis indicated that a single dominant gene was responsible for Karnal bunt resistance. Thirty five inter simple sequence repeat primers were used for amplification of DNA from the two parents. DNA amplification was observed with only 25 markers. ISSR markers based finger print database were generated using these 25 primers. Out of 25, six primers showed 100% polymorphism. A total of 193 alleles were detected at 25 ISSR loci. The number of alleles per locus ranged from 4-14 with an average of 7.72 alleles per locus. The two parents were genetically divers (similarity coefficient 0.419). The cluster analysis led to distribution into two groups: group 1 had resistant parent Aldan and group 2 had susceptible parent WH542, resistant bulk and susceptible bulk. Among all the primers used one primer (IS-43) was identified to be associated with Karnal bunt resistance. This showed an amplification profile (650 bp product) characteristic of resistant parent, in corresponding bulk and in individual genotyping of the recombinant inbred lines. This might have an application in marker assisted selection after converting it to SCAR marker.