Molecular analyses of recombinant inbred lines of wheat cross aldan x WH542 for resistance to Karnal bunt (Neovossia indica)
Loading...
Date
2010
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
Present studies were conducted on two genotypes, Aldan (resistant) and WH542 (susceptible),
and their 94 recombinant inbred lines. Investigations were carried out to screen the recombinant inbred
lines (RILs) for Karnal bunt resistance and to characterize above lines using molecular markers. There
was significant variation among all the recombinant inbred lines for coefficient of infection of Karnal
bunt. The range of coefficient of infection (CI) on the RILs was 0-41.70. The chi-square analysis
indicated that a single dominant gene was responsible for Karnal bunt resistance. Thirty five inter
simple sequence repeat primers were used for amplification of DNA from the two parents. DNA
amplification was observed with only 25 markers. ISSR markers based finger print database were
generated using these 25 primers. Out of 25, six primers showed 100% polymorphism. A total of 193
alleles were detected at 25 ISSR loci. The number of alleles per locus ranged from 4-14 with an average
of 7.72 alleles per locus. The two parents were genetically divers (similarity coefficient 0.419). The
cluster analysis led to distribution into two groups: group 1 had resistant parent Aldan and group 2 had
susceptible parent WH542, resistant bulk and susceptible bulk. Among all the primers used one primer
(IS-43) was identified to be associated with Karnal bunt resistance. This showed an amplification
profile (650 bp product) characteristic of resistant parent, in corresponding bulk and in individual
genotyping of the recombinant inbred lines. This might have an application in marker assisted selection
after converting it to SCAR marker.
Description
Keywords
Diseases, Wheats, Biological phenomena, Dna, Genetics, Genes, Fungi, Polymorphism, Planting, Genotypes