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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Botany × Author: Ashwani Kumar × End date: 2007 × Start date: 2003 × Type: Thesis ×
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    ThesisItemOpen Access
    Biochemical studies during organogenesis in callus culture of ballon vine (Cardiospermum halicacabum L.)
    (CCSHAU, 2006) Ashwani Kumar; Goyal, S.C.
    Present investigation was conducted on Cardiospermum halicacabum L. (Family Sapindanceae) and attempts have been made for direct and indirect regeneration of plantlets from various explants viz., leaf, node and internode from in vivo grown plants. Metabolites, enzymes and protein profiles studies were also made during root and shoot differentiation from callus. All the three explants were cultured on MS medium with and without growth regulators like cytokinins and auxins at different concentrations alone or in combination with each other. All the three explants gave good parcentage of callus induction but the growth of callus was best from leaf explant on MS medium supplemented with 2, 4-D (1.0 mg/l) + Kn (1.0 mg/l). The calli showed differentiation of root on MS + BAP (0.5 mg/l) + NAA (0.3 mg/l) and shoot on MS + BAP (0.5 mg/l) + NAA (0.1 mg/l). Decrease in metabolities like starch, reducing sugars, totals soluble sugar and total phenols while increase in total soluble proteins was found during root and shoot differentiation. Activities of enzymes viz. -amylase acid invertase and peroxidase decreased while acid protease activity increased during root and shoot differentiation. SDS-PAGE studies revealed the synthesis of four common protein ands (51.29, 46.77, 33.8 and 30.2 KDa) during differentiation of root and shoot. It was interesting to note the synthesis of one root specific protein band (85.1 KDa) and two shoot specific proteins bands (102.3 and 36.31 KDa) during differentiation.