Biochemical studies during organogenesis in callus culture of ballon vine (Cardiospermum halicacabum L.)
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Date
2006
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CCSHAU
Abstract
Present investigation was conducted on Cardiospermum halicacabum L.
(Family Sapindanceae) and attempts have been made for direct and indirect
regeneration of plantlets from various explants viz., leaf, node and internode
from in vivo grown plants. Metabolites, enzymes and protein profiles studies
were also made during root and shoot differentiation from callus. All the three explants were cultured on MS medium with and without
growth regulators like cytokinins and auxins at different concentrations alone or
in combination with each other. All the three explants gave good parcentage of
callus induction but the growth of callus was best from leaf explant on MS
medium supplemented with 2, 4-D (1.0 mg/l) + Kn (1.0 mg/l). The calli
showed differentiation of root on MS + BAP (0.5 mg/l) + NAA (0.3 mg/l) and
shoot on MS + BAP (0.5 mg/l) + NAA (0.1 mg/l).
Decrease in metabolities like starch, reducing sugars, totals soluble sugar
and total phenols while increase in total soluble proteins was found during root
and shoot differentiation. Activities of enzymes viz. -amylase acid invertase
and peroxidase decreased while acid protease activity increased during root and
shoot differentiation.
SDS-PAGE studies revealed the synthesis of four common protein ands
(51.29, 46.77, 33.8 and 30.2 KDa) during differentiation of root and shoot. It was
interesting to note the synthesis of one root specific protein band (85.1 KDa) and
two shoot specific proteins bands (102.3 and 36.31 KDa) during differentiation.