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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2010 - 201622
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Subject: Biotechnology and Molecular Biology × End date: 2016 × Start date: 2010 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Molecular mapping of bacterial blight resistance gene, drought tolerant QTL(s) and genetic diversity analysis in clusterbean {Cyamopsis tetragonoloba(L) Taub}
    (CCSHAU, 2011) Bajaj, Anshu; Boora, K.S.
    The present investigation was carried out to screen genetic diversity using RAPD markers and to identify and map the QTL for resistance to bacterial blight and drought tolerance in clusterbean .The plant material was procured from Forage section . For bacterial blight, the resistant (HG 75) & susceptible(PNB) genotypes were selected & crossed to generate F3 population. The F3 population was used to map QTLs. For drought tolerance studies genotypes HG 563 (tolerant) & PNB (susceptible) were used to generate the mapping population (F 2:3).The genetic diversity studies were carried out in 48 genotypes of clusterbean using 130 RAPD primers and 97 primers produced amplification of 705 alleles ;out of which 644 were polymorphic. The similarity ranged from 0.11 to 0.97.The dendrogram grouped the genotypes into two major clusters at a similarity coefficient of 0.68. A variety FS277 was out grouped which is a forage variety. The genotype GP96 and F5277 were found to be most diverse (89%).The QTLs were identified for bacterial blight resistance using parental genotypes (HG75 & PNB) & their F3 population. The F 3 population was screened artificially for disease resistance along with the parent genotypes. Polymorphic primers were used to screen the F3 population & the major QTLs were identified for disease resistance using parental genotypes, HG75, PNB and their F 3 population. A total of 83 RAPD primers were used to screen the parental genotypes out of which nine primers were polymorphic. These primers were used to screen the F 3 population and the QTLs found were L19 , D1, AB7 and QLTY 3. Parental genotypes HG563 and PNB and their F2-3 genotypes were screened at field and net house for resistance to drought. Out of fifty genotypes, 12 were highly tolerant, 23 were moderately tolerant and 15 were susceptible to drought. The parents were screened with eighty seven primers out of which, sixteen were found polymorphic and nine QTLs were found linked to drought tolerance. Loci OPQ 20,OPD10, OPD14,OPQ 12,OPAC 8 and OPF 9 were major contributing QTLs and OPAC2,OPL3 and OPF17 are minor QTLs The diverse lines may be used for breeding programs to improve the production of agronomically superior genotypes of guar. The major QTLs for bacterial blight and drought tolerance may be used for introgression into agronomically superior genotypes of guar.
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    ThesisItemOpen Access
    Molecular analysis of rice (Oryza sativa L.) for bacterial blight resistance genes
    (CCSHAU, 2016) Mehta, Kirti; Shikha Yashveer
    Rice (Oryzasativa) is an important cereal food crop that servesas amajor carbohydrate source for half of the world’s population. Bacterialblight (BB),caused by the bacterium, Xanthomonasoryzaepv. oryzae(Xoo) is one of the most serious threatto the rice production. The present work was undertaken to analyse the major BB resistance genes (Xa21, xa13 and xa5) in the backcrossed populations derived from the crosses between HKR-47 x IRBB-60 and HKR-127 x IRBB- 60 using STS markers. Foreground selection was carried out in theBC3F2plants of both the crossesusing specific STS markers pTA248, RG136 and RG556 linked to Xa21, xa13 and xa5 genes, respectively. A total of sixand nine BC3F2 plantswere found to have all the three resistance genesin the crosses HKR-47 x IRBB-60 and HKR-127 x IRBB-60, respectively. Theseedsfrom these positive plants from both the crosses were harvested separately. Foreground selection of BC3F3 plants of both the crosses was carried out to confirm the presence of three BB resistant genes using the same STS markers.Plants having all the three resistance genes were selected for background selection.Three hundred and sixty SSR markers representing whole of the genome wereused to identify polymorphism between parental genotypes. A total of 77/360 and 79/360 SSR markers produced polymorphism between the parents HKR-47 and IRBB-60 and between, HKR-127and IRBB-60, respectively. Finally, the maximum genome recoveryfor positivegenotypeswas analyzedby NTSYS andGGT softwares. The percentage recovery of recurrent parent genome in three-gene positiveBC3F3 genotypes of the crosses HKR-47 x IRBB-60 and HKR-127 x IRBB-60 ranged from 82.8% to 91.0% and78.6% to 92.1%, respectively. Based on agronomic evaluation, BB reaction, percentage recovery of recurrent parent genome and grain quality evaluation,genotypes G1-8, G1-12 and G1-13from the cross HKR-47 x IRBB-60and genotypes, H1-17and H1-18from the cross HKR-127 x IRBB-60were found promising. The present work successfully analysedthegenotypes from both the crosses forthethree BB resistant genes(Xa21, xa13 and xa5) and recovery of the positive genotypes with maximum recurrent parent genome and other agronomic traits.
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    ThesisItemOpen Access
    Molecular breeding for developing grain protein and micronutrient (Iron and Zinc) dense Indian bread wheat genotypes
    (CCSHAU, 2014) Tomar, Luxmi; Yadav, Neelam R.
    Marker assisted selection strategy was used to introgress the functional Gpc-B1 allele in Indian wheat varieties (WH711 and PBW343) from Lassik, a hard red spring wheat. Fifty two of the most widely accepted Indian varieties were screened for their GPC and micronutrient (Zinc and Iron) content. A narrow range of variability was observed in GPC (9.3-13.7%), zinc (25.8-54.1mg/kg) and iron (37.5-57.9mg/kg) content. H711 and PBW343 were selected and crossed with Lassik. F2 plants were screened for presence/ absence of functional Gpc-B1 and rust resistance genes Lr37/Yr17/Sr38 and Lr34/Yr18 using allele specific amplification (ASA) markers. Plants from F2 observed, on an average 26.4% increase in GPC, 37.5% in zinc and 64.8 % in iron content. The plants did not show any rust susceptibility under field conditions. F2 plants with minimum reduction in yield were chosen for back-crossing with their respective Indian parent. Plants obtained from subsequent back crosses i.e . BC1F1 and BC1F2, also had better quality attributes than the parents. The GPC increased on an average by 30.2% and twice zinc (90.2%) and iron (52.5%) as compared to the recurrent parent. Hybrid plants also had better agronomical properties and showed resistance to stripe rust under field conditions whereas parental genotypes faced significant yield loses. These plants showed complete rust resistance due to presence of Gpc-B1/Yr36 with the Lr37/Yr17/Sr38 and Lr34/Yr18 loci. This population has all the combined benefits of better nutritional quality, yield and disease resistance and could be used both in MAS as well as for transferring the Gpc-B1in other Indian varieties to minimize the linkage drag. Though introgression of Gpc-B1 was linked with a net reduction in yield, interestingly, some of these plants with functional Gpc-B1 allele had higher TKW than either of the parents emphasizing the extent of influence of GxE interaction could have on Gpc-B1 allele. A consensus between quality and quantity has to be reached while breeding for improving GPC in wheat.
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    ThesisItemOpen Access
    Development Of Gender-Specific Scar Marker(S) In Date Palm (Phoenix Dactylifera L.)
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Mitra, Charu; Kharb, Pushpa
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    ThesisItemOpen Access
    Studies On Genetic Diversity And Molecular Mapping Of Qtls For Resistance To Fusarium Wilt And Ascochyta Blight In Chickpea
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Vashist, Urvashi; Boora, Khazan Singh
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    ThesisItemOpen Access
    Phenotypic and molecular analysis for terminal heat stress tolerance in wheat (Triticum aestivum L.)
    (CCSHAU, 2013) Pritam Kumar; Dhillon, Santosh
    The present investigation was conducted to study the phenotypic and molecular analysis of terminal heat stress tolerance in wheat (Triticum aestivum L.) in the Department of Molecular Biology & Biotechnology, CCS Haryana Agricultural University, Hisar, India. Two F2 mapping populations containing 90 plants each involving crosses between Hindi62 (heat tolerant) x WH711 (heat susceptible) and DBW16 (heat tolerant) x WH147 (heat susceptible) were developed. Seeds from individual F2 plants were harvested and sown in two environments, normal sown (30th November 2011) and late sown (26th December 2011) to get F3 mapping population. Heat stress was created by late sowing the crop. Data was recorded on individual plant basis for both the crosses in respect of characters viz. membrane thermostability index, chlorophyll stability index, grain yield, kernels/spike, thousand kernel weight (Test weight), days to heading, days to maturity, plant height, number of tillers/plant, biomass/plant, harvest index, grain filling rate and grain filling duration.103 SSR, 25 RAPD and 2 known gene primers were used in molecular marker analysis for terminal heat stress tolerance. For QTL identification data was analysed by WinQTL-Cartographer. In cross Hindi62 x WH711 three QTL were detected for chlorophyll index at map positions, 135.7 (1B), 184.6 (2A) and 36.4 (6B) by SSR marker WMC 416, Xbarc188, Xgwm526, WMC170, Xgwm219, WMC398respectively. Six QTL were detected for membrane thermostability index at map positions, 118.7 (1B), 58.2 (2A), 137.2 (2A), 176.7 (2A), 62.0 (5A) and 11.7 (6B) by SSR marker WMC416, Xbarc188, WMC407, WMC642, Xgwm356, Xgwm95, Xgwm526, WMC170, Xgwm443, WMC475, Xgwm219 and WMC398. For yield per plant 6 QTLs were detected at map position103.4 (1B), 51.6 (2A), 130.6 (2A), 178.9 (2A), 55.4 (5A), 22.6 (6B). For grain filling duration one QTL was detected at chromosome no. 2A at a map position 171.9 and for grain filling rate 2 QTLs were detected on chromosome 2A and 6B at map position of 66.0 and 76.3 respectively. Similarly in cross DBW16 x WH147, 4 QTLs were detected for chlorophyll index on chromosome 1B, 2A, 6B and 6B respectively. Five QTLs were for membrane thermostability index on chromosome 1A, 1B, 2A, 3A and 6B.One QTL was identified for yield per plant and one for grain filling rate at chromosome 2A and 4A respectively. Five QTLs were identified for grain filling duration at a map position 113.5 (2A), 31.5 (2A), 88.5 (3A), 31.0 (5A) and 29.0(5B). These QTLs may be used for further improvement of the traits they represent. HSP16.9 and HSP 101b primers showed clear distinction between thermotolerant and thermosensitive genotypes and are suggested as potential DNA markers for improving thermotolerance in wheat using marker-assisted selection.
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    ThesisItemOpen Access
    Marker assisted selection for introgression of bacterial blight (BB) resistance genes in rice (Oryza sativa L.)
    (CCSHAU, 2013) Malik, Rekha; Boora, Khazan Singh
    The present investigation was undertaken to introgress the major BB resistance genes (Xa21, xa13 and xa5) into BB susceptible rice variety CSR-30, from BB resistant donor varieties Pusa Basmati -1460 (having two BB resistance genes Xa21 and xa13) and IRBB-60 (having three BB resistance genes Xa21, xa13 and xa5) through marker assisted selection. Crosses were made between CSR-30 x Pusa Basmati -1460 and CSR-30 x IRBB-60. Foreground selection was carried out in F1 plants of both the crosses using specific STS markers pTA248, RG136 and RG556 linked to Xa21, xa13 and xa5, genes respectively. In cross CSR-30 x Pusa Basmati -1460, four F1 seeds were harvested. Foreground selection was carried out in which two plants were found to have both the BB resistance genes Xa21and xa13. These F1 plants were grown in the net house and selfed to get F2 seeds. In cross CSR-30 x IRBB-60, 6 F1 seeds were harvested. Foreground selection was carried out and three plants were found to have three resistance genes Xa21, xa13 and xa- 5. Backcrosses of these positive F1 plants were made with CSR-30 and the BC1F1 seeds of this cross harvested. These BC1F1 seeds were selfed to produce BC1F2 seeds. In foreground selection, 10/250 BC1F2 plants were found to have all the three resistance genes Xa21, xa13 and xa-5. For background selection, 300 SSR primers were used to identify the polymorphism between parental genotypes Pusa Basmati-1460 and CSR-30 and between IRBB-60 and CSR-30, out of which 72 and 104 SSR markers produced polymorphic alleles between the parents Pusa Basmati-1460 and CSR-30 and parents IRBB- 60 and CSR-30, respectively. Cluster tree analysis revealed that the three gene pyramided BC1F2 rice genotypes and the recipient parent CSR-30 fell in one group with two major sub-groups and the donor parent IRBB-60 remained as a separate group. The percentage recovery of recurrent parent genome in the three-gene pyramided BC1F2 genotypes ranged from 44.2% to 78.9% using GGT software. The pyramided lines exhibited very high level of resistance to bacterial blight disease when artificially inoculated with Xanthomonas oryzae strain isolated from the BB infected fields of RRS, Kaul, CCSHAU, Hisar. The agro-morphological traits of the three-gene pyramided BC1F2 genotypes were found to be either superior or on par with the recurrent parent CSR-30. Genotype G-3 showed the best agronomic features with maximum recovery of recurrent parent genome (RPG) 78.9% followed by G-169 with RPG 76.2% followed by line G-7 with RPG 72.5%. The plants having maximum recurrent parent genome were backcrossed with the recurrent parent CSR-30 and BC2F2 seeds were harvested. It is suggested that positive F2 seeds of cross CSR-30 x Pusa Basmati -1460 should be backcrossed with CSR-30 for further work. However, in cross CSR-30 x IRBB-60, BC2F2 genotypes should be further Evaluated for agronomic traits. This work demonstrates the successful application of MAS for targeted introgression of multiple resistance genes into premium quality rice variety CSR-30.
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    ThesisItemOpen Access
    Augmenting The Protein Quality And Mineral Content In Rice (Oryza Sativa L.)
    (Chaudhary Charan Singh Haryana Agricultural University; Hisar, 2010) Basanti; Jain, R K
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    ThesisItemOpen Access
    Development ofRhizobia with improved symbiotic properties for diverse legume crops
    (CCSHAU, 2014) Ikbal; Sikka, Virendra K.
    Present investigation was undertaken to develop efficient rhizobial strains and to over ride host specific symbiotic nodulation behavior in the process of symbiotic infection in diverse legume crops. Such promiscuous Rhizobium strains with improved efficiency to fix nitrogen might add higher amount of nitrogen to the plants. Towards these efforts 295 rhizobial strains were isolated from nodules of legumes chickpea, pea and lentil grown in different fields. These isolates were then analyzed for their cellulose and pectin utilizing ability by growing them on CMC and pectin media. Best growing strains on CMC medium were selected for gamma irradiation to create genetic variations. These gamma irradiated mutant isolates were screened for their enhanced cellulose and pectin utilizing ability. These mutants were compared with their parents for enhanced cellulose and pectin utilization ability. It was found that percent increase in growth on CMC media over parents was in the range of 8.82 to 422.22%. Rhizobial mutants having contrasting antibiotic profile were electrofused for obtaining cross infective rhizobia. Total five fusants each from the rhizobial cross combination E1, E2, E3, E4 and E5 which had their resistance pattern confirmed were obtainedwith the help of electroporation. Symbiotic infection behaviour and cross infectivity of electrofusants, mutants and wild type rhizobial isolates were analysed on chickpea, pea and lentil plants through plant infection test. It was found that doses of gamma-rays on biofertilizer appeared significant effect on number of nodules, plant weight and shoot N content. Greater number of nodules and promiscuous behavior was observed in mutants followed by electrofusants as compare to their parental rhizobial isolates.Mutant rhizobial strains improved shoot nitrogen content up to 50% inchickpea,45% in pea plant whereas the same trend was also observed on lentil plants. The developed Rhizobium strains with improved symbiotic association and ability to infect across strict specificity for host legumes would be of great help for the farming community at large. Representative strains were selected for the molecular analysis based on PCR amplification. As a nodulation genes marker, nodA, nodC, nodD, nodE, nodV and nodW genes were selected, which are common nod genes essential for nodulation in all rhizobial species investigated so far and these are also determinants of host range. The comparison of the nod genes between symbiotic strains and host plant groups taken together indicated that lateral transfer of Sym genes and genetic rearrangements are involved in the genetic modification.Apparently the Nod factors of the strains are quite variable. The PCR amplification of these factors determined that these factors were considerably altered through mutation and rearrangements were taken place by electro-poration.