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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Biotechnology and Molecular Biology × Author: Chaudhary, Anuya × Start date: 2005 × Type: Thesis ×
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    ThesisItemOpen Access
    Micropropagation of guggal (Commiphora mukul, hook ex stock)
    (CCSHAU, 2005) Chaudhary, Anuya; Yadav, R.C.
    The present study was undertaken to develop an efficient micropropgation system for plant regeneration and transfer the regenerated plants to soil conditions in Guggal (Commiphora mukul, Hook ex stock). Nodal, shoot tip and leaf explants were used for shoot regeneration on 37 different media supplemented with different growth regulators and additives. Nodal explants gave best shoot regeneration response (71.40%) on MS medium supplemented with kinetin (1.0 mg/l) and GA3 (1.0 mg/l) and MS medium fortified with BAP (5.0 mg/l) and GA3 (1.0 mg/l) gave highest per cent of shoot initiation (81.11%) in shoot tip explants. The shoots were elongated on MS medium containing different concentrations of growth regulators using agar as gelling agent. Best shoot elongation was observed on MS medium containing BAP (5.0 mg/l) and GA3 (1.0 mg/l) out of the four media tried. Shoot tip explants are considered to be the best explant compared to nodal explants for shoot regeneration. Leaf explants did not show any response. However, callusing was observed on MS medium containing 2,4-D (1.0 mg/l). Analysis of variance of shoot formation indicated that nature of explants, medium and their interaction were highly significant at 1 per cent level of significance. Rooting of regenerated shoots was observed (35.00%) on liquid MS medium supplemented with IBA (1.0 mg/l) out of fifteen different media tried. The regenerated plantlets were transferred to soil and vermculite mixture (1:1) and 30 per cent plants survived in the soil.