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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Biotechnology and Molecular Biology × Author: Alka Rani × Start date: 1980 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Molecular characterization of cluster bean (Cyamopsis tetragonoloba L. Taub.) genotypes
    (CCSHAU, 2019) Alka Rani; Yadav, Neelam R.
    Cluster bean (Cyamopsis tetragonoloba L. Taub.) is an annual, diploid legume crop with 14 number of chromosomes and highly valued for presence of galactomannan in its seeds. For commercial use of seeds, their genetic purity determination is very important. The present study was carried out for development of molecular profile of commercial varieties of cluster bean using SSRs and to study molecular diversity in selected genotypes of cluster bean using EST-SSRs. For this study, seeds of 22 commercial varieties and 41 germplasm lines of guar were grown in net house of the department and Farm area of Forage Section, Department of Genetics and Plant Breeding. DNA was extracted using CTAB method. Molecular analysis was carried out using 79 SSRs which included 40 EST-SSRs and 39 miSSRs from Medicago truncatula and cluster bean (developed in our lab). Out of 79 SSRs, 55 SSRs were found polymorphic and employed for varietal identification. Among these polymorphic SSRs, eight SSRs identified selected varieties. Ct-miSSR-17, Ct-miSSR-19, Mt-miRNA-SSR45, Mt-miRNA-SSR47 and Mt-miRNA-SSR164, EST-SSR2, EST-SSR17 and EST-SSR40 identified selected varieties. Among 55 polymorphic SSRs, size of amplified products ranged from 100-1160 bp and PIC value ranged from 0.28 to 0.91. The molecular analysis divided the 22 cluster bean varieties into two major clusters at similarity coefficient of 0.06 and HG 6 existed as an isolated genotype. Cross-transferability of Medicago truncatula miRNA-SSRs showed 31% cross-transferability among cluster bean varieties. Molecular analysis showed that IC 402295-2, IC 522486, IC 113452, IC 415161 and IC 113489 were extreme and diverse group with around 30 % gum content while IC 113472 and IC 370725 were low gum (<20%) genotypes. The molecular analysis of 63 genotypes together showed that all the 22 varieties are in different genetic pool than 41 germplasm lines and can be exploited in cluster bean breeding programme.