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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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Subject: Biochemistry × Author: Jyoti × Start date: 1998 × Type: Thesis ×
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    ThesisItemOpen Access
    In vitro antioxidant and antimicrobial activities of mustard extracts
    (CCSHAU, Hisar, 2021-09) Jyoti; Nisha Kumari
    Mustard is the third most important oilseed crop after oil palm and soybean. Although mustard is widely used as edible oil and condiment, the residue left after oil extraction is popularly used as livestock and poultry feed with excellent protein quality. However, the information regarding antioxidative and antimicrobial potential of mustard seed meal is still scanty. Therefore, the objective of the present study was to extract the polyphenols from brown and yellow mustard seed meal with different solvents i.e 80% methanol, 80% ethanol, 80% acetone and 80% isopropanol. Each extract was analysed for antioxidant potential using DPPH and ABTS scavenging activity, ferric ion reducing antioxidant power (FRAP) assay, total antioxidant activity, iron chelating activity and β-Carotene content. The 80% acetone extracted maximum polyphenols followed by 80% ethanol, 80% methanol and 80% isopropanol. Brown mustard exhibited a higher amount of polyphenols as compared to yellow mustard. Yellow mustard exhibited higher antioxidant potential in terms of DPPH and ABTS scavenging activity, ferric ion reducing antioxidant power, iron chelating activity and β-Carotene content. Correlation analysis indicated a negative association between total phenolic content and DPPH, ABTS and FRAP. Antimicrobial potential was evaluated against two gram-negative (Escherichia coli and Xanthomonas campesteris), two gram positive (Bacillus cereus and Lactobacillus plantarum) bacteria and three fungi (Fusarium oxysporum, Macrophomina phaseolina and Candida albicans). Yellow mustard exhibited higher antibacterial activity while brown mustard had higher antifungal activity. Both the mustard seed meal extracts were ineffective against probiotic bacteria Lactobacillus plantarum. Gallic acid, caffeic acid, ferulic acid and sinapic acid were identified in mustard seed meal by HPLC analysis.
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    ThesisItemOpen Access
    Carbohydrate metabolism in developing seeds of Brassica campestris L.
    (College of Basic Sciences and Humanities Chaudhary Charan Singh Haryana Agricultural University Hisar, 1999) Jyoti; Singh, Randhir
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    ThesisItemOpen Access
    Use of molecular markers for varietal identification and assessing phylogenetic status of Basmati rice in genus Oryza sativa L.
    (CCSHAU, 2011) Jyoti; Jain, Sunita
    Molecular markers provide novel tools for varietal identification, diversity analysis and assessing phylogenetic relationships among various rice groups in genus Oryza. A set of 50 rice genotypes comprising of seven japonica rice varieties, six traditional Basmati, thirteen cross-bred Basmati and 24 indica rice varieties was investigated using 54 markers (28 on chromosome 8 and 26 on rest of the chromosomes); data obtained has been used to assess the phylogenetic status of Basmati rice in genus Oryza sativa L. A total of 217 alleles were detected, with an average of 4.01 alleles per locus. Number of alleles per locus (3.5 alleles) for markers on chromosome 8 was less than the mean value (4.58 alleles) based on 26 SSR markers on rest of eleven chromosomes of rice. Eleven of these alleles were unique, present in only one genotype. Null allele was observed at only one locus (RM408) in Super Basmati. The number and size of alleles and Polymorphism Information Content (PIC) values ranged between 2-7, 83-381 bp and 0.074-0.810, respectively. An average PIC of 0.55 per locus was obtained, which confirms that markers used in this study were highly informative. Genetic relationships generated using the whole genome marker data as well as allelic profile on chromosome 8 and rest of eleven chromosomes separately placed 50 rice genotypes in three distinct clusters. Seventeen of the nineteen Basmati rice varieties (except two cross-bred Basmati rice varieties, Sabarmati and Improved Sabarmati) formed a separate cluster quite distinct from the indica and japonica rice clusters. Interestingly, mean similarity indices obtained from chromosome 8 dataset placed Basmati rice cluster at equal distance from japonica and indica rice types. However, whole genome as well as rest of the genome marker datasets placed Basmati group closer to indica rice varieties than japonica. Chromosome 8 dataset showed a positive correlation (Mantel test, r = 0.631) with the rest of the genome dataset, indicating a higher level of similarity between the two. All the traditional and crossbred Basmati rice varieties and two aromatic japonica varieties shared the same BAD2 alleles at the aroma locus, indicated the distinctness of the aroma locus. While the present study gives support to the hypothesis that japonica genotypes may have contributed towards the evolution of chromosome 8 or a part of chromosome 8 in Basmati rice, it also indicate the active flow of genes from indica rices during the course of parallel evolution in northern Indian sub-continent. The study also demonstrates the distinctness of TB from other rice types (indica and japonica) and also provides a number of novel SSR markers which can be used to differentiate within/among the various rice groups at commercial level. It was observed that the amplified products differing in ≥12bp can be separated on 3% agarose gels for varietal identification in Basmati rice, which is faster, much cheaper and relatively simple method compared to the advanced molecular methods already available for the purpose.
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    ThesisItemOpen Access
    Use Of Molecular Markers For Warietal Identification And Assessing Phylogenetic Status Of Basmati Rice In Genus Oryza Sativa L.
    (Department Of Biochemistry College Of Basic Sciences And Humanities CCS Haryana Agricultural University : Hisar, 2010) Jyoti; Jain,Sunita.