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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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2009 - 200912010 - 20161
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Subject: Biochemistry × Author: Babita Rani × Start date: 1985 × Type: Thesis ×
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    ThesisItemOpen Access
    Effect of arbuscular mycorrhiza fungi on biochemical parameters in wheat (Triticum aestivum L.) under drought conditions
    (CCSHAU, 2016) Babita Rani; Madan, Shashi
    The present study was carried out to evaluate the effect of arbuscular mycorrhizal fungi (AMF) on the biochemical changes of two wheat varieties viz. WH 1025 and WH 1105. Plants of two wheat varieties were raised in earthen pots. Water stress was created by withholding irrigation at two growth stages (jointing and heading). In present study the effect of drought stress was observed on antioxidative system, nitrogen, phosphorous metabolizing enzymes, physiological, quality and yield parameters at jointing and heading stage. The results showed that under stress conditions, mycorrhizal inoculation significantly increased the relative water content, chlorophyll content and chlorophyll fluorescence but decreased the osmotic potential and electrolyte leakage in leaves of both the varieties at both the growth stages. The AMF colonized plants had significantly lower superoxide radical (O2 .-) hydrogen peroxide (H2O2) and malondialdehyde (MDA) content as compared to the uninoculated controls under water deficit conditions in leaves and roots of both the varieties. The activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and glutathione reductase enhaced at both growth stages in both the varieties under these conditions. However the higher activities were reported in WH 1025 under stress conditions. Contrarily, reduction in CAT activity was observed in leaves of WH 1105 at both the stages. Ascorbic acid and glutathione content were found to be increased in WH 1025, however decreased in WH 1105 under stress conditions. Further, increase in nitrogen and phosphorous metabolizing enzymes under stress conditions is in agreement with N, P, K levels at both the growth stages of WH 1025 and WH 1105 varieties. Quality parameters like moisture content and crude fiber content decreased whereas, sedimentation value, gluten, protein content and grain hardness increased under drought stress conditions however AMF inoculation did not play a significant role in quality traits at both the growth stages of both the varieties. Yield parameters like grain number per spike, grain weight, numbers of productive tillers and biomass per plant and grain yield decreased under drought stress while in AMF treated plants yield was more than untreated plants of both varieties. The reduction in physiological, biochemical, yield and grain quality traits was found to be more pronounced in WH 1105 in comparison to the WH 1025. These results demonstrated that the AMF could confer greater tolerance of WH 1025 variety to soil water deficit through an enhancement in their antioxidative defence system and thereby reducing the level of O2 .-, H2O2 and MDA content
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    ThesisItemOpen Access
    High temperature induced changes in oxidative stress, antioxidant system and polypeptide pattern in Indian mustard [Brassica juncea (L.) Czern & Coss.]
    (CCSHAU, 2009) Babita Rani; Dhawan, Kamal
    The present investigations were undertaken to study the changes in lipid peroxidation, antioxidative enzymes, metabolites and protein pattern in Brassica juncea seedling after subjecting to high temperature stress (45±0.5C) and on revival. Two tolerant genotypes viz. BPR-542-6 and NRCDR-02 and two susceptible genotypes viz. NPJ-119 and RGN-152 were screened at 45±0.5C on the basis of time taken to 50% seedling mortality. Lipoxygenase (LOX), malondialdehyde (MDA) and hydrogen peroxide (H2O2) which are indicators of cell membrane damage increased under heat stress in all the genotypes but increase was higher in susceptible genotypes. On recovery, LOX, MDA and H2O2 content decreased in all the genotypes. The activities of antioxidative enzymes viz . superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) increased under high temperature stress . SOD and CAT started decreasing but activity of POX and GR still continued increasing in all the genotypes. But APX enzyme exhibited differential behaviour on revival which increased in tolerant genotypes but decreased in susceptible genotypes. Ascorbic acid and carotenoids increased under heat stress but during recovery, ascorbic acid continued increasing while carotenoids started decreasing in all the genotypes. A major band of 53.12 kDa and minor bands of 100 kDa, 89.12 kDa, 74.13 kDa, 46.76 kDa and 38.9 kDa in tolerant genotypes and protein bands of molecular weight 25.79 kDa and 30.7 kDa in susceptible genotypes appeared under high temperature stress which disappeared when the stress was relieved.