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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    “Assessment of differential gene expression of various cytokines due to in vitro exposure of selected medicinal plant extracts in chicken splenocytes”
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-10) Shivani; Ambwani, Sonu
    Immune system is crucial for poultry health and productivity. The permutation in immunity may result in increase or decrease of immune response, known as Immunomodulation. Nowadays, use of ethnomedicinal plants and their products for modulation of immune response has become the preferred approach. The present study focused on the exploration of antioxidative, anti- inflammatory and immunomodulatory potential of the three plant extracts (PEs) viz., aqueous leaf extract of Moringa oleifera (MOE), hydromethanolic leaf extract of Eclipta alba (EAE) and aqueous root extract of Bergenia ligulata (BLE). The maximum non-cytotoxic dose (MNCD) of the MOE, EAE, BLE and DEXA was determined through MTT assay and immunomodulatory effect of all the PEs and DEXA individually as well as in combination of the PEs and DEXA was evaluated through Lymphocyte Proliferation Assay (LPA). Moreover, in vitro differential gene expression at mRNA level was evaluated in chicken splenocytes by treating them with the MNCD of MOE, EAE, BLE, DEXA individually as well as in combinations of the PEs and DEXA via quantitative real time PCR (qRTPCR). A significant in vitro antioxidant and anti-inflammatory activity was exhibited by all the three PEs with highest potential in case of MOE followed by EAE and BLE, respectively. The MNCD of all the three PEs and DEXA showed 100% cell viability as determined through MTT assay. Moreover, PEs displayed a significant increase in B and T cells proliferation in LPS, PHA, Con A treated splenocytes. Over all the three PEs displayed significant immunopotentiating properties while DEXA showed immunosuppressive effect due to in vitro exposure in chicken lymphocytes. The differential gene expression analysis showed significant alteration in the expression levels due to in vitro exposure of MOE, EAE, BLE, DEXA and combination of PEs and DEXA. There was a significant increase in transcription factor NFAT1, NFAT2, AP-1c-Fos, AP-1 c-Jun and cytokines like IL-2, IL-4 and IFN-􀁊 that triggered immunostimulatory effect due to PEs exposure, whereas DEXA displayed immunosuppressive potential in chicken splenocytes. There was significant increase in antioxidant and anti-inflammatory mediators (Nrf-2 and IL-10) while significant down regulation in pro-inflammatory mediators (IL-1β, IL-6, TGF-β, TNF-α, iNOS2, COX-2 and NF-κB1) due to in vitro exposure of PEs and DEXA in chicken splenocytes. Combination treatment exhibited ameliorative effect of PEs against DEXA induced immunosuppression in LPA as well as in differential gene expression. Over all the results showed antioxidative, anti-inflammatory and immunostimulatory potential of all the three PEs in vitro whereas DEXA displayed the immunosuppressive and anti- inflammatory property. The PEs may further be explored through suitable in vitro and in vivo analyses to use these PEs as feed additive for improved poultry health and performance.
  • ThesisItemOpen Access
    Development of biofortified oyster mushroom (Pleurotus spp.) using Zinc Sulphate nanoparticle
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-11) Shivani; Kushwaha, K.P.S.
    Pleurotus spp. commonly known as oyster mushroom in english and Dhingri in hindi, is one of the most important edible mushrooms cultivated in all around the world and is rich in nutritional as well as medicinal properties. Zinc is an essential trace element next to iron and its deficiency can lead to growth retardation, loss of appetite, and impaired immune function. Since the human body does not store excess zinc, it must be consumed regularly as part of the diet. Biofortification of food crops for increasing the zinc content and its bio-availability is one of the potential alternatives for combating zinc deficiency. The present study pertains to the development of zinc bio-fortified Pleurotus spp. (P. sajor-caju, P. florida and P. flabellatus) using zinc sulphate nanoparticles (ZnSO4 NPs). Zinc sulphate nanoparticles was sprayed at different concentrations (10 ppm, 20 ppm, 30 ppm and 40 ppm) after complete spawn run and nutritional and antioxidant properties of the fruiting bodies were analysed. The maximum mycelial radial growth and growth rate per day (mm) was obtained in all the tested species of Pleurotus at 10 ppm concentration of ZnSO4 NPs. Moreover, there was maximum increase in the growth parameters (pileus width, stipe length and stipe width), total yield and biological efficiency was obtained at10 ppm concentration of ZnSO4 NPs of as compared to the control. The highest increase in zinc content was obtained in 40 ppm concentration of ZnSO4 NPs i.e., 115.63% in P. sajor-caju followed by P. flabellatus (113.58%) and P. florida (106.35%). Similarly, at 40 ppm concentration of ZnSO4 NPs the phenol and flavonoid content was also found maximum. Whereas, the highest sodium and potassium content was bioaccumulated at 10 ppm concentration. The nutritional status of Pleurotus spp., measured in terms of crude protein, total ash and total soluble sugars, was found highest at 10 ppm concentration. The maximum increase in DPPH, ABTS and FRAP radical scavenging activity was observed at 10 ppm concentration of zinc sulphate nanoparticle. In case of, DPPH radical scavenging activity the highest increase was observed in P. sajor-caju followed by P. flabellatus and P. florida. Whereas, in case of ABTS radical scavenging activity the highest increase was obtained in P. sajor-caju followed by P. florida and P. flabellatus. Furthermore, in case of FRAP radical scavenging activity the highest increase was obtained in P. flabellatus followed by P. sajor-caju and P. florida. Therefore, Pleurotus spp. when biofortified with zinc sulphate nanoparticle at 10 ppm concentration has positive effect on the mycelial growth, biological efficiency, growth parameters, bioaccumulation of micronutrients, nutritional as well as antioxidant activities of Pleurotus spp. Thus, 10 ppm ZnSO4 NPs can be used for getting maximum yield of oyster mushroom and to combat zinc deficiency.
  • ThesisItemOpen Access
    Combining ability and heterosis analysis for yield and drought related traits in chickpea (Cicer arietinum L.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-07) Shivani; Arora, Anju
    The present investigation was undertaken to identify promising parents and crosses through Line × Tester analysis involving eight lines (PG 3, PG 4, PG 5, PG 114, PG 186, GNG 1581, CSJ 515 and GNG 1958) and three testers (ICC 4958, ICC 16350 and ICC 16351) for yield and drought related physiological traits based on GCA and SCA effects. Heterosis was also estimated to identify superior combinations. Crosses and parents were evaluated for different eleven agronomic traits (days to 50 % flowering, days to maturity, plant height, number of primary branches per plant, number of secondary branches per plant, number of pods per plant, number of seeds per pod, 100 seed weight, biological yield per plant, seed yield per plant and harvest index) and eight drought related traits viz. relative water content, chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, proline content, membrane stability index and canopy temperature depression at 50% flowering and pod formation stages. Analysis of variance for yield and related traits was found significant for all the eleven traits studied. The analysis of variance for combining ability in yield related traits indicated that both additive and non additive gene actions were important for expression of the traits. The estimates of 2 sca were found to be higher than 2 gca for all the traits except for plant height and number of pods per plant. The parents PG 186, GNG 1581 and CSJ 515 were found to be promising general combiners for maximum number of yield and related traits. The crosses GNG 1581 × ICC 4958 and CSJ 515 × ICC 4958 were identified as potential crosses based on significant SCA effects and heterosis over mid, better and standard parents. Analysis of variance for drought related physiological traits was found significant for all the traits except carotenoid content at 50% flowering stage. Analysis of variance for combining ability indicated significant Line × Tester results for all the eight traits at both the stages. The estimates of 2 sca were found to be higher than 2 gca for all the traits examined at both the stages, indicating good prospects for exploitation of non-additive genetic variance for physiological traits. The parents PG 3, PG 5 and PG 186 were found to be promising general combiners for maximum number of drought related physiological traits including proline content, relative water content, at either 50% flowering or pod formation stages. The crosses GNG 1958 × ICC 4958 and PG 186 × ICC 16351 were identified as potential crosses based on significant SCA effects and estimation of heterosis over mid, better and standard parents at both the stages. Overall, PG 186 was the most promising parent for higher yield as well as drought tolerance traits. GNG 1581 × ICC 4958 was the promising cross for yield and drought related traits in chickpea based on significant SCA values and estimates of heterosis over mid, better and standard parents.
  • ThesisItemOpen Access
    Isolation of plant growth promoting rhizobacteria from soils of north- western himalayan region of Uttarakhand, their characterization and evaluation in vegetable pea (Pisum sativum)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-05) Shivani; Raverkar, K.P.
    The studies were conducted to isolate various plant growth promoting rhizobacteria from the rhizosphere and rhizoplane of vegetable pea by raising it in soils of different locations of North-West Himalayan region of Uttarakhand; their characterization and evaluation for improved growth of vegetable pea under controlled conditions. The microbiological and chemical analyses were carried out in the Central Analytical Laboratory and Department of Soil Science, College of Agriculture, GBPUA&T, Pantnagar (Uttarakhand) during 2016-2018. Total of forty-three isolates were isolated and purified from the rhizosphere and rhizoplane of vegetable pea grown in the soils from varying locations of the North-West Himalayan region of Uttarakhand. Further these isolates were characterized on the basis of morphological, biochemical characteristics and plant growth promoting (PGP) traits. Out of forty-three rhizobacterial isolates 29, 25, 35, 37 and 32 tested positive for amylase, protease, catalae, oxidase, and chitinase, respectively. However, 32.55, 13.95, 9.30, 69.76, 67.44 and 100 percent isolates were P-solubilizer, Zn-soubilizer, HCN producer, IAA synthesizer, GA synthesizer and ammonia producer, respectively. These rhizobacterial isolates also expressed the great diversity in terms of carbohydrate utilization. On the basis of the PGP traits expressed by various isolates 30 were selected for evaluating their potential to improve growth and development of vegetable pea in sand under controlled conditions. After 45 days of plant growth i.e. at the time of flowering PGPR isolates A17-RP-KB (T7) produced maximum shoot length (40.24 cm), root length (20.62 cm), lateral root emergence (61.17 number plant-1), dry root biomass (1.06g plant-1), total dry weight (2.11g plant-1) and root: shoot ratio (1.01). The highest root volume (7.3 cm3 plant-1), fresh root weight (9.023 g plant-1), fresh shoot weight (7.87 g plant-1), shoot: root ratio (2.84) and dry shoot biomass (1.13 g plant-1) was produced due to an inoculation with PGPR isolates LG-RS-1-KB (T8), A15-RP-KB (T28), SH-RP-NA (T15), PL-RS-1-NA (T16) and P2-RP-KB (T1), respectively. Inoculation with PGPR isolate CRC-RP-NA (T20) facilitated synthesis of higher amount of chlorophyll ‘a’; 14.23 mg g-1) and ‘b’ ( 6.44 mg g-1) while the highest leaf area (5.35cm2 plant-1) was produced due to LG-RS-1-KB (T8). Total N and P-uptake of 28.22 and 2.51 a l p g m t n -1 R P G P h t i w n o i t a l u c o n i o t e u d t s e h g i h s a w , a e p e l b a t e g e v n i , y l e v i t c e p s e r ,7 isolate A1 -RP-KB (T7). However, highest amount of total K-uptake (32.41 mg plant-1) was recorded in plants inoculated with PGPR isolate CRC-RS-2-NA (T6). The highest root acid phosphates activity (12.76 mg p-nitrophenol g-1 fresh root hr-1) was demonstrated due to inoculation with PGPR isolate SH-RS-2-KB (T24).
  • ThesisItemOpen Access
    Characterization of elite lines of bread wheat for rust (stripe and leaf) and powdery mildew resistance
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-06) Shivani; Vishunavat, Karuna