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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Studies on the prevalence of gastrointestinal parasites in the chicken of U.S.Nagar district of Uttarakhand and efficacy of certain herbal formulations against ascaridiosis
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-07) Yadav, Mukesh Kumar; Singh, V.S.
    The present study was carried out to investigate the prevalence of gastrointestinal parasites in backyard and commercial chickens as well as to determine the comparative efficacy of standard drug and certain herbal formulations against Ascaridiosis in naturally infected backyard chicken reared in U.S. Nagar, district of Uttarakhand for a period one year from June, 2018 to May, 2019 whereas efficacy trial was evaluated for 28 day from 2, March, 2019 to 30 March 2019. A total of three thousand three hundred and thirty five (3335) samples comprising of 1618 (1392 droppings and 226 gastrointestinal tract) from backyard and 1717 (1180 droppings and 537 gastrointestinal tract) commercial chickens were collected randomly from eight (8) tehsils (Kichha, Rudrapur, Sitarganj, Kashipur, Gagarpur, Bazpur, Jaspur and Khatima) of U.S. Nagar, district of Uttarakhand. However, efficacy trial were conducted in seventy five backyard chickens, randomly divided into 5 groups, each groups have 15 birds of 3 replicates and each replicates have 5 naturally infected chicken with ascaridiosis. Chickens of Group I was kept as Control and untreated. Chickens of Group II were treated with piperazine @ 80mg/kg body wt orally for 3 consecutive days. Chickens of Group III were treated with a combination of Neem, Papaya, Vaividang and Bathua powder (25% each) @ 2gm/kg body wt. Chickens of Group IV were treated with a combination of Bathua and Vaividang powder (50% each) @ 2gm/kg body wt while those of group V were treated with Bathua powder 2gm/kg body wt for 3 consecutive days. Collected sample were examined for gastrointestinal parasites using standard techniques. Eight (8) different gastrointestinal parasite eggs and oocytes were identified. Out of these parasites, four species were related to nematodes such as Ascaridia galli, Heterakis gallinarum, Capillaria spp., Trichostrongylus spp: three species were of cestodes such as Raillietina spp., Cotugnia sp. and Hymenolepis sp. and none of the chicken was infected with trematodes. In gastrointestinal protozoa, Eimeria spp. were recorded. Prevalence of different species of gastrointestinal helminths was higher in backyard chicken (60.44%)) as compare to commercial (18.25%). However prevalence of Eimeria spp. was higher in commercial chicken (62.02%) than backyard (33.62%). In backyard chicken as well as comercial chicken, higher overall prevalence of nematodes (24.46%) were found as compare to cestodes (8.57%) and mixed infection (5.42%). Seasonal prevalence of helminths and Eimeria spp. was recorded highest in Rainy followed by summer and Winter, in both backyard and comercial chicken. In monthly data analysis, maximum prevalence was recorded in August and least in Januay. In vivo trials revealed that Ascaridia galli infection caused significant decrease in the body weights of chicken. Group II was found to be most effective in which weight gain was increased 9.79 % of the initial body weight. This was further followed by group III, IV and V increasing 7.41, 6.51, and 6.02 % of the initial body weight. However, in efficacy trial, EPG count were found significantly decreased (p<0.05) in all treated groups and significantly (p<0.05) increased in control group during the whole experimental period. Group II was found to be most effective (100%) at 7th day onward. This was further followed by group V with 21.07, 56.01, 67.12 and 83.04% reduction in EPG on days 7, 14, 21 and 28 days, respectively. Group III showed minimum effect among the herbal formulation against feacal egg reduction count with 12.87, 45.90, 64.88 and 77.89% on days 7, 14, 21, and 28th days, respectively. Signicant increase in fecal egg reduction counts was recorded in group I (control) with 3.30, 6.06, 8.97 and 14.10% on 7, 14, 21, and 28 day respectively. Comparative study of morphology of Ascaridia galli and Heterakis gallinarum was carried out by scanning electron microscopy and light electron microscopy. In male, caudal end A.galli has precloacal sucker, two equal sized spicules emerged from anal opening and ten pairs of caudal papillae. However, Female had a simple straight tail with a ventrally located anal opening. Caudal end of male Heterakis gallinarum have well developed preclocal sucker, two unequal sized spicules emerged from cloacal opening and 12 pairs of caudal papillae. Whereas tail of female was straight, long; narrow and pointed. Therefore, prevalence study may be helpful to develop strategies for treatment and control of these gastrointestinal parasites of chicken in particular study area. Herbal formulations may be used as moderate anthelmintics against ascariodiosis.
  • ThesisItemOpen Access
    Prevalence and molecular characterization of cystic echinococcosis in buffaloes
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-06) Sofi, Omer Mohi u din; Vatsya, Stuti
    The present investigation was undertaken to study the prevalence of cystic echinococcosis (CE) in buffaloes slaughtered at slaughter houses in Udham Singh Nagar and Nainital districts of Uttarakhand state for a period of one year from January, 2017 to December, 2017. Of the 322 animals screened, the overall prevalence rate of 12.11% of CE was observed {Udham Singh Nagar district (n=150) - 10% (lungs=26.67%, liver=53.33% and liver + lung=20%); Nainital district (n=172) - 13.95% (lungs=33.33%, liver=50% and liver + lung=16.66%)}. Overall, the average prevalence of 30.76%, 51.28% and 17.94% was recorded in liver, lung and liver + lung, respectively with significantly (P<0.05) higher prevalence of cystic echinococcosis in lungs as against liver. Seasonal prevalence of CE revealed significantly (P<0.05) higher prevalence of CE in winter (15.38%) than summer (11.49%) and rains (10%). Different types of cysts were recovered from various infected organs with significantly (P<0.05) different rates of infection {single cysts (lungs=80%, liver=66.66%), multiple cysts (lungs=20%, liver=33.33%)}. The fertility rate of 39 hydatid cysts examined from total number of slaughtered buffaloes was found to be 61.53% {sterile=25.65%, calcified=12.82%} and significantly higher fertility rate of cysts from lungs (65%) was observed as against liver (58.33%) and lung + liver (57.14%). The overall viability rate of protoscolices that were recovered from cysts of all slaughtered animals was observed to be 83.33% {lungs (76.92%) and liver (71.42%)}. A significant (P<0.05) difference of the viability of protoscolices of different sizes of the cysts was also observed {<3cm (0%), 3-6cm (75%) and >6cm (93.33%)}. Micrometric study (Mean±SE) of large and small hooks of protoscolices was performed by taking seven 7 parameters viz. total hook length (TL), blade length (BL), blade width (BW), blade guard distance (BGD), handle length (HL), handle width (HW) and total width (TW) {n=25; large hooks (TL=21.66±0.71μm, BL=13.96± 0.62μm, BW=3.94± 0.47μm, BGD=10.99± 0.51μm, HL=7.05± 0.51μm, HW=3.28± 0.46μm, TW=7.15± 0.54μm), small hooks (TL=17.23± 0.55μm, BL=9.23± 0.46μm, BW=2.25±0.41μm, BGD=7.16± 0.45μm, HL=7.44± 0.33μm, HW=3.29± 0.52μm, TW=6.12± 0.47μm)}. Overall length and handle length of massive and small hooks of buffalo isolates were observed to be substantially higher. Both total length and handle length of large and small hooks could be used as the most potential characters for brief identification of parasites in epidemiological studies. DNA was extracted from overall 28 samples (protoscolices and laminated layer). The DNA and cox1 gene amplification fragment length of all the isolates was found to be 18kb and 493bp, respectively. The cox1 gene sequence obtained from Udham Singh Nagar and Nainital isolate showed 100% and 99.9% identity with India, Sudan and Brazil isolates (G5 genotype), respectively which confirmed the prevalence of G5 genotype of Echinococcus granulosus in buffaloes in the study area of Uttarakhand. The nucleotide variation in the cox1 gene sequence as compared to Brazil isolate resulted in change in the translated amino acid sequence at only 1 site for Udham Singh Nagar isolate (at position 7, Serine was replaced by Alanine) and at 2 sites for Nainital isolate (at position 98, Serine was replaced with Leucine and at position 112, Cysteine was replaced by Serine). When Udham Singh Nagar isolate was compared with the Nainital isolate, change in the amino acid sequence was observed at 2 sites (at position 98, Leucine was replaced with Serine and at position 112, Serine was replaced by Cysteine). Phylogenetic analysis of cox1 gene obtained from Udham Singh Nagar and Nainital districts of Uttarakhand revealed that the Udham Singh Nagar and Nainital isolates originated from same ancestor and both had maximum relevance with G5 genotype from Brazil isolates. This is the first report of G5 genotype of CE in buffaloes from Uttarakhand and thus is even more significant in understanding its zoonotic potential.
  • ThesisItemOpen Access
    Studies on prevalence of bovine tropical theileriosis and assessment of its economic impact
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Nagar, Ankit; Rajeev Ranjan Kumar
    Considering the economic importance and increase reports of Bovine Tropical Theileriosis (BTT), the prevalence of the disease in district of Udham Singh Nagar, Uttarakhand was studied host wise, area wise, season wise and month wise from July, 2017 to June, 2018. alongwith evaluation of chemical drugs against Theileria annulata in cattle and assessment of its economic impact. A total of 694 blood samples of bovines (541 from cattle and 153 from buffaloes) were examined and found 338 animals (48.70%) were positive for BTT. Maximum (54.34%) prevalence of BTT was observed in cattle and than in buffaloes (28.75%). Area-wise prevalence of BTT in cattle revealed maximum (100%) in Sitarganj area and the lowest (50%) in Kashipur area. However, in buffaloes, it was recorded maximum (39.39%) in Kichha and the lowest (0%) in Sitarganj areas. Seasonal prevalence of BTT in cattle was recorded maximum (63.73%) in summer followed by rainy (60%) and the lowest (45.62%) in winter season. In buffaloes also it was recorded highest in summer season followed by rainy and then in winter with infection rate of 40.67%, 25.58% and 17.64%, respectively. Monthwise prevalence of BTT in both cattle and buffaloes revealed maximum prevalence in the month of May with infection rate of 70.58% and 47.05%, respectively. However, it was recorded minimum (41.53%) in the month of February in cattle and 0% during January and February in buffaloes. Haematological examination revealed a significant (P<0.05) decrease in haemoglobin concentration (6.28±0.42 g%), packed cell volume (19.14±1.20 %), total erythrocytic count (3.98±0.21 × 106/μl) and increase in TLC (9.31±0.37 × 10³/μl) were recorded in infected animals. However, it was in uninfected group were 10.29±0.38 g%, 31.19±1.09 %, 6.02±0.19 × 106/μl and 8.37±0.24 × 10³/μl, respectively. Significant (P<0.05) lymphopenia (51.90±4.30 %) and neutrophilia (36.00±4.89 %) were observed in infected cattle. However, differential leucocytes count revealed 60.90±1.52 % lymphocytes and 34.80±1.58 % neutrophils in control animals. The increase in monocytes (5.50±1.00 %) and eosinophils (6.60±2.50 %) were observed in infected cattle as compared with uninfected control monocytes (1.80±0.40 %) and eosinophils (2.50±0.57 %). A decrease in MCV (48.04±1.81 fl) and MCH (15.73±0.54 pg) were observed in infected group as compared to control (MCV-51.81±0.77fl and MCH 17.08±0.26 pg) which revealed microcytic RBC’s and low average quantity of haemoglobin present in single erythrocyte, respectively. However, no changes was observed in MCHC (32.78±0.37 g%) in infected group. The anti-theilerial efficacy of various combinations of chemical drugs (buparvaquone +oxytetracycline, chloroquine + oxytetracycline, α,β-Artether + Oxytetracycline and diminazine aceturate +oxytetracycline) was also studied in cattle naturally infected with T. annulata. Buparvaquone showed better efficacy than diaminazine aceturtae, chloroquine and alpha, beta-arteether when used in combination with oxytetracyline at recommended dose. It reduces the intensity of infection from ++++ to + as compared to other drugs but unable to eliminate the parasite from blood of infected animals. The effect of treatment on milk production revealed a total increase of 50.05litre milk yield/animal following treatment with buparvaquone and supportive drugs over a period of 28days. The total economic losses due to BTT were estimated of Rs. 789.85 per animal. On the basis of present study, it can be concluded that prevalence data may be used for formulating an effective control strategies against Bovine Tropical Theileriosis in animals of district Udham Singh Nagar. The findings of evaluation of various chemical drugs suggest that two doses of bupravquone are essentially required for complete elimination of the parasite from the blood of infected animals. The disease is fatal in nature, so that it is suggested that the farmers should go for proper diagnosis of the disease and consult Veterinarians for minimizing the economic losses.
  • ThesisItemOpen Access
    Studies on evaluation of some phytoacaricides against Rhipicephalus (Boophilus) microplus
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Vijay Kumar; Vatsya, Stuti
    A study was under taken with objective of detecting synthetic pyrethroid resistance (deltamethrin concentrations- 0.0003125%, 0.000625%, 0.00125%, 0.0025%, 0.005%, 0.01% and 0.02%-each tested in triplicate) in R. microplus using two in vitro laboratory bioassays namely Adult Immersion Test (AIT) and Larval Packet Test (LPT) collected from different parts of Uttarakhand state {Nainital (Bindu Khatta, Patwadangar, Halduchor), Udham Singh Nagar (Saanp Katani Khatta, Pantnagar Dairy, Nehru Colony, Sitarganj), Pithoragarh (Bin) and Uttarkashi} to generate a baseline data and Azadirachta and Eucalyptus plants were screened for acaricidal activity. The susceptible tick population collected from Bindu Khatta, Nainital displayed an LC50 of 0.00075% Active Ingredient (A.I.) [Confidence Interval (C.I.) 95% 0.0006-0.0009] and LC99 of 0.00533% (C.I. 95% 0.0034-0.0112) in AIT bioassay. The R. microplus population from Nehru colony exhibited the highest LC50 of 0.03192%, (CI 95% 0.0188-0.1026%) followed by Pithoragarh (0.00526%, CI 95% 0.0036-0.0083%), Pantnagar dairy (0.00514%, CI 95% 0.0036-0.0080%), Sitarganj (0.00376%, CI 95% 0.0025-0.0061%), Halduchor (0.00337% CI 95% 0.0025-0.0047%), Saanp Katani Khatta (0.00189% CI 95% 0.0013-0.0026%), Nainital (Patwadangar) (0.00177% CI 95% 0.0012-0.0025%) and Uttarkashi (0.00088%, CI 95% 0.0007-0.0011). The R2 values of 0.994, 0.991, 0.978, 0.974, 0.973, 0.964, 0.962, 0.952 and 0.877 were detected in Bindu Khatta, Uttarkashi, Pithoragarh, Pantnagar Dairy, Nehru colony, Nainital, Halduchor, Sitarganj, and Saanp Katani Khatta populations, respectively. The Nehru colony population of ticks had the highest resistance factor (RF) of 42.55, whereas the Pithoragarh and Pantnagar dairy populations had RF values of 7.01 and 6.85, respectively; Sitarganj, Halduchor, Saanp Katani Khatta and Nainital tick populations were 5.01, 4.49, 2.52, and 2.36 times resistant, respectively. Level IV resistance was found in Nehru colony tick population; level II in Pithoragarh, Pantnagar Dairy, Sitarganj and level I in Halduchor, Saanp Katani Khatta and Nainital R. microplus populations. The population of ticks collected from Uttarkashi and Bindu Khatta were found susceptible to deltamethrin. In LPT bioassay, an LC50 of 0.00074% A. I. (C.I. 95% 0.0005-0.0010) was observed for the Susceptible R. microplus population. The LC50 values were observed to be (0.03524%, CI 95% 0.0248- 0.0607%), (0.00508%, CI 95% 0.0037-0.0073%), (0.00439%, CI 95% 0.0030-0.0067), (0.00393%, CI 95% 0.0021-0.0089%), (0.00365%, CI 95% 0.0025- 0.0054%), (0.00196%, CI 95% 0.0013-0.0029%), (0.00165% CI 95% 0.0012-0.0022%) and (0.00097% CI 95% 0.0006-0.0015) for Nehru colony, Pantnagar Dairy, Sitarganj, Pithoragarh, Halduchor, Saanp Katani Khatta, Nainital and Uttarkashi, respectively. Azadirachta indica and Eucalyptus citriodora were selected to know their acaricidal potential against R. microplus ticks. The % yield obtained with, aqueous, methanolic and aqueous-methanolic extract was, 16, 7.48 and 17.56 for A. indica and 13.28, 18.96 and 19.28 for Eucalyptus. The results of AIT using the aqueous, methanolic and aqueous-methanolic extract of both plants revealed a dose dependent decrease in reproductive index (RI) and percent inhibition of oviposition (IO%) generally from concentration 2.5 to 20%. A decrease in egg production was significant (p<0.05) for 2.5, 5, 10 and 20% concentration of the extract. Tick mortality percent of 40 and 70% was observed at 10 and 20 % concentration of methanolic extract solution of A. indica. The results of AIT with E. citriodora revealed that RI and IO% decreased from concentration 1.25 to 20%. The results of AIT using the Neem oil showed that there was dose dependent decrease in RI and IO% in concentrations ranging from 1.25 to 20%. A decrease of 5, 30, 40, 50 and 60% hatching of eggs was observed at 1.25, 2.5, 5, 10 and 20% concentration of oil, respectively. With Eucalyptus oil, also a dose dependent decrease in RI and IO% for 1.25% concentration was noted. A decrease of 50% hatching of eggs was also observed at 1.25% concentration of oil. Ticks failed to oviposit at 2.5% to 20% concentration. 100% mortality was observed at 2.5% to 20% concentration of oil. In LPT bioassay, an LC50 of 9.333% A. I. (C.I. 95% 6.132-16.983) was observed for the Azadirachta aqueous extract solution against resistant R. microplus population. With E. citriodora aqueous methanolic solution, LC50 of 9.620% A. I. (C.I. 95% 4.491-17.148), methanolic LC50 of 14.492% A. I. (C.I. 95% 13.210-16.060) and aqueous LC50 of 17.556% A. I. (C.I. 95% 2.916.210-18.817)}. E. citriodora oil exhibited LC50 of 11.634% and neem oil an LC50 of 22.818% against larvae of R. microplus population. In LIT, no mortality of R. microplus larvae was recorded at any concentration of A. indica and E. citriodora extracts except at 20% concentration of neem aqueous solution (85%). Several phytoconstituents were revealed on phytochemical analysis of A. indica [alkaloids, glycosides, saponins, sterols, tannins and triterpenes] and E. citriodora [alkaloids, flavonoids, reducing sugar, saponins, tannins and triterpenes]. Based on results of laboratory bioassays, it is possible to conclude that the oils of A. indica and E. citriodora caused a negative effect on reproduction and hatchability against deltamethrin resistant R. microplus while Eucalyptus oil even resulted in mortality of ticks. The use of these oils shows great potential for the future as an alternative biocontrol method for R. microplus. These can be incorporated as part of an integrated control programme for ticks.
  • ThesisItemOpen Access
    Prevalence of gastro-intestinal parasites in zoo animals of some Indian zoos
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-07) Niranjan, Anuruddha Singh; Singh, Vidya Sagar
    The present research was conducted to study the prevalence and intensity of gastrointestinal parasites in the Zoo animals at Zoological Park, Nainital and Kanpur. A total of 333 faecal samples (99 samples from Nainital, Zoo and 234 samples from Kanpur, Zoo) were randomly collected. The overall prevalence of parasitic infection were 28.28% and 32.05% whereas, prevalence of helminths, protozoans and mixed infection were 20.20%, 7.07%, 1.01% and 20.94%, 8.11%, 2.99% for Zoological Parks, Nainital and Kanpur respectively. The results for Zoological Park, Nainital indicated that the helminth infections were more compared to protozoans and mixed infection in herbivores, carnivores and some birds. The prevalence of helminths and protozoans in carnivores were 20.83%, 4.16% respectively and for herbivores were 27.77%, 16.66% and mixed infection 5.55% respectively. Gastrointestinal parasites (eggs/cysts/oocysts) identified in herbivores were Strongyle sp., Capillaria sp. (11.11%), Trichuris sp., Muellerius sp. (5.55%) and Eimeria sp. (16.66%). EPG/CPG/OPG ranged from 100 to 500. Gastrointestinal parasites (eggs/cysts/oocysts) identified in carnivores were Toxocara cati (12.50%), Toxocara canis (8.33%) and Isospora sp.(4.16%). EPG/CPG/OPG ranged from 150 to 450. Common Peafowls were found positive with Ascaridia galli (18.18%) and Eimeria sp. (3.63%). The results for Zoological Park, Kanpur indicated that the helminth infections were more than protozoan and mixed infection in herbivores, carnivores, omnivores and non-human primates. The prevalence of helminths, protozoans and mixed infection in carnivores were 34.42%, 6.55% and 3.27% respectively, for herbivores, 16.93%, 4.83% and 3.22% repectively, for non-human primates 13.33% and 20%. Gastrointestinal parasites (eggs/cysts/oocysts) identified in herbivores were Amphistome (2.41%), Fasciola sp. (1.61%), Trichuris sp. (3.22%), Strongyle sp., Strongyloides sp., Oesophagostomum sp., Trichostrongylus sp.(2.41%) and Eimeria sp. (4.83%). EPG/CPG/OPG was ranged from 50to350. Gastrointestinal parasites (eggs/cysts/oocysts) identified in carnivores were Ancylostoma sp. (8.19%), Toxocara cati (8.19%), Strongyloides sp. (6.55%), Trichuris sp. (4.91%) Toxocara canis (3.27%), Toxascaris leonina (1.63%), Spirometra sp. (1.63%) and Isospora sp. (6.55%). EPG/CPG/OPG were ranged from 100 to 600. Gastrointestinal parasites (eggs/cysts/oocysts) identified in non-human primates were Strongyloides sp. (13.33%) and Eimeria sp. (20%). EPG/CPG/OPG were ranged from 200 to 350. The overall prevalence of intestinal parasitic infection in the present study was 30.93% with 21.02% samples positive with helminths, 7.80% samples positive with protozoans and 2.10% samples positive with mixed infection. The overall prevalence of helminths, protozoans and mixed infection in herbivore were 18.30%, 6.33% and 3.52%, in carnivores were 30.58%, 5.88% and 2.35%, in non-human primates helminths and protozoan prevalence were 12.76% and 21.27% and in wild birds were 18.18% and 3.63% respectively. The overall prevalence of helminths in omnivores was 0.60%. There was no protozoan and mixed infection. Looking to the incidence of gastro-intestinal parasites kept in captivity it can be concluded that there is a need for detailed epidemiological investigation on the prevalence of gastrointestinal parasites by regular screening of faecal samples in Zoo animals with respect to season, age, climate etc. Based on the prevalence of parasites deworming with suitable anthelmintic is required. Proper hygienic measures for the control of different stages of parasites need to be taken care of so as to reduce the parasitic infection in Zoo animals.
  • ThesisItemOpen Access
    Studies on evaluation of some phytoacaricides against Rhipicephalus (Boophilus) microplus
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2017-08) Vijay Kumar; Vatsya, Stuti
    A study was under taken with objective of detecting synthetic pyrethroid resistance (deltamethrin concentrations- 0.0003125%, 0.000625%, 0.00125%, 0.0025%, 0.005%, 0.01% and 0.02%-each tested in triplicate) in R. microplus using two in vitro laboratory bioassays namely Adult Immersion Test (AIT) and Larval Packet Test (LPT) collected from different parts of Uttarakhand state {Nainital (Bindu Khatta, Patwadangar, Halduchor), Udham Singh Nagar (Saanp Katani Khatta, Pantnagar Dairy, Nehru Colony, Sitarganj), Pithoragarh (Bin) and Uttarkashi} to generate a baseline data and Azadirachta and Eucalyptus plants were screened for acaricidal activity. The susceptible tick population collected from Bindu Khatta, Nainital displayed an LC50 of 0.00075% Active Ingredient (A.I.) [Confidence Interval (C.I.) 95% 0.0006-0.0009] and LC99 of 0.00533% (C.I. 95% 0.0034-0.0112) in AIT bioassay. The R. microplus population from Nehru colony exhibited the highest LC50 of 0.03192%, (CI 95% 0.0188-0.1026%) followed by Pithoragarh (0.00526%, CI 95% 0.0036-0.0083%), Pantnagar dairy (0.00514%, CI 95% 0.0036-0.0080%), Sitarganj (0.00376%, CI 95% 0.0025-0.0061%), Halduchor (0.00337% CI 95% 0.0025-0.0047%), Saanp Katani Khatta (0.00189% CI 95% 0.0013-0.0026%), Nainital (Patwadangar) (0.00177% CI 95% 0.0012-0.0025%) and Uttarkashi (0.00088%, CI 95% 0.0007-0.0011). The R2 values of 0.994, 0.991, 0.978, 0.974, 0.973, 0.964, 0.962, 0.952 and 0.877 were detected in Bindu Khatta, Uttarkashi, Pithoragarh, Pantnagar Dairy, Nehru colony, Nainital, Halduchor, Sitarganj, and Saanp Katani Khatta populations, respectively. The Nehru colony population of ticks had the highest resistance factor (RF) of 42.55, whereas the Pithoragarh and Pantnagar dairy populations had RF values of 7.01 and 6.85, respectively; Sitarganj, Halduchor, Saanp Katani Khatta and Nainital tick populations were 5.01, 4.49, 2.52, and 2.36 times resistant, respectively. Level IV resistance was found in Nehru colony tick population; level II in Pithoragarh, Pantnagar Dairy, Sitarganj and level I in Halduchor, Saanp Katani Khatta and Nainital R. microplus populations. The population of ticks collected from Uttarkashi and Bindu Khatta were found susceptible to deltamethrin. In LPT bioassay, an LC50 of 0.00074% A. I. (C.I. 95% 0.0005-0.0010) was observed for the Susceptible R. microplus population. The LC50 values were observed to be (0.03524%, CI 95% 0.0248- 0.0607%), (0.00508%, CI 95% 0.0037-0.0073%), (0.00439%, CI 95% 0.0030-0.0067), (0.00393%, CI 95% 0.0021-0.0089%), (0.00365%, CI 95% 0.0025- 0.0054%), (0.00196%, CI 95% 0.0013-0.0029%), (0.00165% CI 95% 0.0012-0.0022%) and (0.00097% CI 95% 0.0006-0.0015) for Nehru colony, Pantnagar Dairy, Sitarganj, Pithoragarh, Halduchor, Saanp Katani Khatta, Nainital and Uttarkashi, respectively. Azadirachta indica and Eucalyptus citriodora were selected to know their acaricidal potential against R. microplus ticks. The % yield obtained with, aqueous, methanolic and aqueous-methanolic extract was, 16, 7.48 and 17.56 for A. indica and 13.28, 18.96 and 19.28 for Eucalyptus. The results of AIT using the aqueous, methanolic and aqueous-methanolic extract of both plants revealed a dose dependent decrease in reproductive index (RI) and percent inhibition of oviposition (IO%) generally from concentration 2.5 to 20%. A decrease in egg production was significant (p<0.05) for 2.5, 5, 10 and 20% concentration of the extract. Tick mortality percent of 40 and 70% was observed at 10 and 20 % concentration of methanolic extract solution of A. indica. The results of AIT with E. citriodora revealed that RI and IO% decreased from concentration 1.25 to 20%. The results of AIT using the Neem oil showed that there was dose dependent decrease in RI and IO% in concentrations ranging from 1.25 to 20%. A decrease of 5, 30, 40, 50 and 60% hatching of eggs was observed at 1.25, 2.5, 5, 10 and 20% concentration of oil, respectively. With Eucalyptus oil, also a dose dependent decrease in RI and IO% for 1.25% concentration was noted. A decrease of 50% hatching of eggs was also observed at 1.25% concentration of oil. Ticks failed to oviposit at 2.5% to 20% concentration. 100% mortality was observed at 2.5% to 20% concentration of oil. In LPT bioassay, an LC50 of 9.333% A. I. (C.I. 95% 6.132-16.983) was observed for the Azadirachta aqueous extract solution against resistant R. microplus population. With E. citriodora aqueous methanolic solution, LC50 of 9.620% A. I. (C.I. 95% 4.491-17.148), methanolic LC50 of 14.492% A. I. (C.I. 95% 13.210-16.060) and aqueous LC50 of 17.556% A. I. (C.I. 95% 2.916.210-18.817)}. E. citriodora oil exhibited LC50 of 11.634% and neem oil an LC50 of 22.818% against larvae of R. microplus population. In LIT, no mortality of R. microplus larvae was recorded at any concentration of A. indica and E. citriodora extracts except at 20% concentration of neem aqueous solution (85%). Several phytoconstituents were revealed on phytochemical analysis of A. indica [alkaloids, glycosides, saponins, sterols, tannins and triterpenes] and E. citriodora [alkaloids, flavonoids, reducing sugar, saponins, tannins and triterpenes]. Based on results of laboratory bioassays, it is possible to conclude that the oils of A. indica and E. citriodora caused a negative effect on reproduction and hatchability against deltamethrin resistant R. microplus while Eucalyptus oil even resulted in mortality of ticks. The use of these oils shows great potential for the future as an alternative biocontrol method for R. microplus. These can be incorporated as part of an integrated control programme for ticks.
  • ThesisItemOpen Access
    Studies on evaluation of efficacy of herbal plants against gastrointestinal nematodosis in goats
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-06) Sastya, Sushmita; Rajeev Ranjan Kumar