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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Genetic divergence, character association and seed vigour testing in jatropha (Jatropha curcas L.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2014-08) Joshi, Vipin Chandra; Nautiyal, M.K.
    The present study was initiated in the year 2005 under the project National Network on Integrated Development of Jatropha and the present study carried out during the year 2011-14. The genotypes were planted in Randomized block design (RBD) with three replication at Medicinal Plant Research and Development centre (MRDC) of G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India. the observations were recorded on seed yield plant-1 (kg), seed yield hactare-1(kg), plant height (cm), collar diameter (cm), days to flower initiation, total number of branches plant-1, seed index (g), to study the genetic divergence among the different Jatropha genotypes. among them five superior genotypes are studied for seed vigour testing in same year side by side under lab and field conditions by applying different days of accelerated ageing treatments, the experiment was laid out in factorial CRD with three replication. After accelerated ageing the seed quality parameters of Jatropha genotypes under lab conditions were recorded. The seed aged from different treatments of each variety were subjected to field experiment for evaluating plant growth, seed yield and its contributing characters. The analysis of variance revealed that there was significant differences among all the forty six genotypes for all the characters except seed index in 2011-12, thee pooled data over two years showed highly significant differences in treatment x years and treatments for all the traits. IGAU- Raipur showed maximum seed yield hactare-1, & seed yield plant-1, collar diameter & seed index followed by IGAU- Bilaspur. A close study of PCV, GCV & ECV indicated high amount of variability for seed yield plant-1, seed yield hactare-1, & days to flower initiation where as collar diameter, total number of branches plant-1, and seed index showed moderate amount of variability high heritability accounted for seed yield plant-1, seed yield hactare-1(kg), collar diameter (cm), days to flower initiation, total number of branches plant-1, indicating less contribution of environment. Moderate heritability estimates was found for plant height as well as phenotypic correlation with seed yield hectare-1, collar diameter, number of branches plant-1. D2 analysis of the pooled data over two years represents all the forty six genotypes are grouped in eight clusters. Out of eight clusters highest no of genotypes are found in cluster-II. The maximum intra cluster distance (57.86), was exhibited by cluster-II indicating scope of selection. The maximum inter cluster distance (318.54) between cluster I & VIII, indicating wide genetic diversity between the genotypes. In seed vigour testing under laboratory conditions every treatment had significant negative influence over un aged control in all the parameters. Out of five ageing treatments 144h, ageing period showed greater effect of ageing followed by 120, 96, 72,48h, ageing respectively. The genotype IGAU- Raipur had more capable to maintain seed vigour after all ageing and considered vigorous and good storer under ambient conditions. Whereas Pant-J03103, TNMC-3, demonstrate as poor vigour and storability. In field experiment the results correlated with laboratory experiments.
  • ThesisItemOpen Access
    Evaluation of fennel (Foeniculum vulgare Mill.) germplasm for growth and yield characters under tarai conditions of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2011-08) Chander Mohan; Singh, J.P.
  • ThesisItemOpen Access
    DUS characterization of maize (Zea mays L.) and French bean (Phaseolus vulgaris L.) varieties by using morphological, biochemical and molecular markers
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2011-08) Mall, Neetu; Chawla, H.S.
    The present study was carried out on 24 maize and 11 French bean varieties with the objectives: i) To provide a detailed description of morphological DUS descriptors for maize and French bean varieties over two years as per the DUS guidelines; ii) To determine the relative extent of distinctiveness, uniformity and stability of morphological DUS descriptors; iii) Characterization of the varieties by biochemical and molecular markers to complement the morphological DUS descriptors. The field experiments for maize and French bean were conducted in the two kharif seasons and two Rabi seasons of 2008-2009 and 2009-2010 at Crop Research Centre and Vegetable Research Centre respectively. Data were recorded for 31 and 22 morpho-physiological DUS descriptors for maize and French bean respectively. Among 31 descriptors of maize, 19 were visually assessed, 4 neither visual nor measurable and 8 were measurable characteristics. 1 character was monomorphic, 12 were dimorphic and 5 were polymorphic for visual characters. All neither visual nor measurable characters were dimorphic. Out of 8 measurable characteristics 1 was monomorphic, 5 were dimorphic and 2 were polymorphic. Among 19 visual characters of French bean, 13 were dimorphic and six were polymorphic. All the 3 measurable characters were polymorphic indicating their potential for varietal characterization. No intra-varietal variation was observed for any of the visual characteristics except for one character in French bean and expression of characters in different varieties remained same for two consecutive years confirming the uniformity and stability of the varieties. COY-D analysis was made on 8 and 3 measurable DUS descriptors of maize and French bean respectively. COYD analysis supported with MJRA analysis revealed that the slope of the MJRA curve and regression probability were too negligible which indicated that all the considered characteristics were independent and their interactions with environment as well as with themselves were negligible in both the years respectively. This indicates the distinctiveness of all the candidate varieties. COY-U analysis revealed that all the varieties were more or less uniform for measurable characters. However, 2 varieties in maize and six varieties in French bean requires further purification for one more year. PCV and GCV showed greater magnitude of differences in maize which indicates less stability however in French bean high heritability indicated the presence of additive gene action in the expression of characters, emphasizing their consistency and stability over the years and their utility in varietal characterization. On the basis of grouping characteristics unique morphological profiles were obtained for 14 maize and 3 French bean varieties. When all the morphological descriptors were considered then all the varieties of maize were distinguished and seven varieties of French bean were discriminated. Seed storage protein profiling of varieties discriminated six varieties by SDS-PAGE in maize and four varieties in French bean. Molecular marker analysis was conducted with two markers viz. RAPD and SSR in maize and SSR in French bean. RAPD and SSR analysis with 10 primers each exhibited high level of polymorphism. Unique specific bands were obtained for fourteen varieties with RAPD marker and 8 varieties in maize with SSRs marker. When both the molecular markers were considered, a total of 16 varieties could be discriminated in maize. SSRs in French bean with 10primers revealed very high degree of polymorphism. Distinctive profiles were obtained for four varieties. When biochemical and molecular markers were considered then overall nine varieties were discriminated out of 11 varieties in French bean. Thus, DUS descriptors were found reliable and reproducible for establishing the distinctiveness of the varieties but sometimes morphological DUS descriptor alone may not be sufficient for establishing the distinctiveness of varieties. However, when supplemented with different biochemical and molecular markers majority of the varieties could be delineated. The varieties satisfying the DUS criteria can be registered with PPV&FR authority for obtaining breeders and Farmer’s rights.
  • ThesisItemOpen Access
    DUS characterization on morphological, biochemical and molecular basis in Brassica juncea L.Czern & Coss and Brassica carinata A. Braun
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2014-02) Nitin Kumar; Pant, D.P.
    The present study was carried out with 26 mustard varieties including 16 Indian mustard and 10 Ethiopian mustard varieties over two rabi seasons of 2011-12 and 2012-13.Data were recorded for 24 morphological DUS descriptors (8 visually assessed and 16measurable). In Indian mustard among the eight visually assessed DUS descriptors, only one was monomorphic, three were dimorphic and four were polymorphic, while in Ethiopian mustard four were monomorphic, only one was dimorphic and three were polymorphic. In Indian mustard out of 16 measurable characteristics, three were monomorphic, eight were dimorphic and five were polymorphic. In Ethiopian mustard seven characteristics were monomorphic and dimorphic respectively and two were polymorphic. COYD analysis with respect to 16 measurable traits indicated that all the varieties were absolutely distinct from each other in case of Indian mustard and Ethiopian mustard. COYD analysis was supported with MJRA analysis in which the slope of the MJRA curve and regression probability was calculated, which indicated that characteristics under study were not completely independent rather they are interacting with each other as well as with the environment. COYU analysis revealed that all the varieties were more or less uniform for measurable characteristics in ecotypes. Statistical analysis for stability, comparing respective values of PCV and GCV based on morphological data showed same magnitude for twelve measurable characteristics in Indian mustard and nine characteristics in Ethiopian mustard. This indicated that environmental influence was less for the expression of these characters. High heritability coupled with high to moderate genetic advance for most of the measurable characteristics indicating their consistency and stability over the years and their utility in varietal characterization. On the basis of grouping characteristics none of the Indian mustard and Ethiopian mustard varieties could establish distinctiveness by unique morphological profiles. However, all the varieties of Indian mustard and Ethiopian mustard were discriminated when all the morphological descriptors were considered. SDS-PAGE protein profiling of mustard varieties revealed moderate level of polymorphism. Only NDRE-4 and PRB-2006-5 variety of Indian mustard could establish distinctiveness when clustering was done using the UPGMA method on the basis of banding pattern scoring. Molecular marker analysis was conducted with SSRs. Twenty SSR primers produced unique banding pattern in three Indian mustard varieties Kranti, Krishna and PRB-2006-5 and two Ethiopian mustard varieties Kiran Early and PBC-2009-3. Thus, morpho-physiological DUS descriptors were found reliable and reproducible for establishing the distinctiveness of the varieties under study. Sometimes morphological DUS descriptors alone may not be sufficient for establishing distinctiveness of varieties, but when supplemented with biochemical and molecular markers varieties could be discriminated. The varieties and those strains satisfying the DUS criteria could be register to utilize with PPV and FR Authority for breeders and farmers rights and also in crop improvement programme.
  • ThesisItemOpen Access
    Studies on biochemical and physiological parameters of seed quality in relation to ageing in oilseed brassicas
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2013-07) Bhim Jyoti; Ram Bhajan
    Deterioration in seed quality during ageing is a natural phenomenon. This is caused by alterations in various physiological/ biochemical parameters such as seed germination, seedling length, seed vigour increased in seed leachate, decreased of seed viability and dehydrogenase activity. Changes in these parameters adversely affect seed yield and related characters. A series of laboratory and field experiments were, therefore, conducted in the Department of Genetics and Plant Breeding and at the NEB Crop Research Center, G.B. Pant University of Agri. & Tech. Pantnagar, Distt. U.S. Nagar (Uttarakhand) from Jan 2010 to March 2012 to elucidate the effect of ageing periods on physiological, biochemical parameters, and seed yield and related traits in rapeseed-mustard. The material consisted of three oilseed Brassicas- toria (Brassica rapa var toria), yellow sarson (B. rapa var yellow sarson) and mustard (B. juncea L) and four varieties each of these namely PT-303, PT-507, T-9, and Bhawani of toria; Ragini, B- 9, PPS-1, and NDYS-2 of yellow sarson; and Vardan, Varuna, Kranti and NDRE-4 of mustard stored for one, two, three and four years in cloth bags under ambient conditions. Results showed decline in all the three physiological parameters with increase in ageing period. Significant reduction in all physiological and biochemical parameters were observed in 3 and 4 years old seeds. Maximum decline in physiological parameters was found in yellow sarson than mustard and toria. At varietal level, significant differences were observed for all physiological parameters. Rapid decline in physiological parameters was observed in PT-303 variety of toria, Ragini of yellow sarson and Varuna of mustard whereas, Bhawani of toria, B-9 of yellow sarson and Kranti of mustard showed less reduction for all physiological traits than other three varieties. At biochemical level, significant differences were observed due to crops and their varieties during ageing. In general, as ageing period increased, viability and dehydrogenase activity decreased but seed leachate increased. Significantly high leakage of seed leachate was observed in yellow sarson than mustard and toria. Less pronounced effect of seed viability and dehydrogenase activity during ageing was found in toria than mustard and yellow sarson. Significantly higher leakage of seed leachate accompanied by more reduction in seed viability and dehydrogenase activity was found in PT-303 of toria, Ragini of yellow sarson, and Varuna of mustard than other three varieties. Less reduction in these traits was found in Bhawani in toria, B-9 in yellow sarson, and Kranti in mustard. SDS-PAGE showed non-significant alterations in protein profiling during ageing for crops as well as varieties. Seed ageing delayed flowering more in mustard followed by in toria and yellow sarson. Higher reduction in seed yield was observed in yellow sarson than mustard and toria. At varietal level higher reduction in seed yield was observed in PT-507 of toria, Ragini of yellow sarson, Varuna of mustard. Bhawani of toria, B-9 of yellow sarson and Kranti of mustard showed less reduction in seed yield than other three varieties. On the basis of physiological, biochemical parameters and yield related traits it was inferred that seed of toria and mustard could be stored for two years without reduction in germination % under ambient condition. Toria was good storer than other two crops. Yellow sarson lose viability and vigour after one year ageing, may be due to high oil content, thin transparent seed coat and high leakage of seed leachate. At varietal level, Bhawani in toria, B-9 in yellow sarson, and Kranti in mustard showed better storability than others studied. Irrespective of crops, brown and small seeded types were less sensitive to ageing than yellow and bold seeded varieties.
  • ThesisItemOpen Access
    Studies on heterosis, combining ability and seed quality parameters in cucumber (Cucumis sativus L.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2014-01) Samant, Anuradha; Singh, Dinesh Kumar
    The present investigation was carried out at Vegetable Research Centre during 2011-12. Assessment of seed vigour parameters and biochemical characterization was performed in the NAIPLaboratory, Department of Vegetable Science, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India. The major objectives of this study were to know variability parameters, heritability, genetic advance; extent of heterosis, combining ability in crosses between genotypes of cucumber along with study of seed quality parameters and biochemical characterization. Analysis of variance revealed highly significant among the genotypes for fourteen characters, which clearly indicated that there was enough variability for these traits among the genotypes utilized for present investigation. The best parents identified as promisingly general combiner were PCUC-126, PCPGR-4273 and Pant Khira-1. The good specific combiners were PCUC-117 x PCUC-208, PCUC-117 x PCUC-133, PCPGR-4273 x PCUC-126 and PCUC-59 x Pant Khira-1 for different character. These hybrids are also noted for having significantly higher SCA effects for yield contributing character which culminated in higher total yield. The cross PCUC-117 x Pant Khira-1 exhibits higher heterobeltiosis and standard heterosis for earliness. For yield contributing characters the crosses PCUC-117 x PCUC-133, PCUC-117 x PCUC-208, PCPGR-4273 x PCUC-104 recorded highest heterobeltiosis and standard heterosis. For seed yield the crosses PCUC-133 x PCUC-208, PCPGR-4273 x PCUC-133, PCUC-117 x Pant Khira-1 recorded highest heterobeltiosis and standard heterosis. Further study of seed quality parameters and establishment of its genetic purity is one of the pertinent issues resurgent in present times. In addition to this a series of laboratory tests were also conducted involving eight pure line genotypes and twenty-eight hybrids to determine the effect of abiotic stresses (moisture and salt) on seed quality parameters. Under moisture stress and salt stress test, it was found that germination per cent, seedling length and vigour index decreased significantly as level of stress increased. The vigour parameters revealed PCPGR-4273, Pant Khira-1, PCUC-126 and PCUC-59 as superior pureline genotypes and PCUC-126 x PCUC-117, PCUC-126 x Pant Khira-1 PCUC-126 x PCUC-133 PCUC-117 x PCUC-59 as superior hybrids. The seed protein profile of parental genotypes revealed twenty-one protein bands which were divided into different zones and subzones according to their light and dark bands.
  • ThesisItemOpen Access
    Development and analysis of morpho-physiological DUS descriptors and assessment of genetic divergence in oat (Avena sativa L.)
    (G.B. Pant University of Agriculture & Technology, Pantnagar - 263145 (Uttarakhand), 2014-09) Rawat, Naveen Singh; Verma, J.S.
    The present study was carried out with 21 oat varieties over two seasons of Rabi 2010-11 and Rabi 2011-12. Data were recorded for 33 morpho-physiological characteristics (21 visually assessed and 12 measurable). Among the twenty one visually assessed characteristics, three were monomorphic, three were dimorphic and fifteen were polymorphic. Out of 12 measurable characteristics, two were monomorphic, three were dimorphic and seven were polymorphic. The COYD analysis with respect to 12 measurable traits indicated that all the varieties were clearly distinct from each other. COYD analysis was supported with MJRA analysis where the slope of the MJRA curve and regression probability was calculated, which indicated that characteristics under study were not completely independent rather they are interacting with each other as well as with the environment. The COYU analysis revealed that all the varieties were more or less uniform for measurable characteristics. Statistical analysis for stability, comparing respective values of PCV and GCV based on morphological data showed same magnitude for seven measurable characteristics. This indicated that environmental influence was less for the expression of these characters. High heritability coupled with high to moderate genetic advance for most of the measurable characteristics indicating their consistency and stability over the years and their utility in varietal characterization. Out of the 21 oat varieties under study, 14 varieties could establish distinctiveness by unique morpho-physiological profiles on the basis of proposed grouping characteristics (six). The seven remaining varieties (OL-9, SKO-20, Sabzar, JO-03-91, JO-1, JHO-99-1 and HJ-8) were discriminated further when certain morpho-physiological characteristics i.e., primary grain: hairiness of base, primary grain: length of lemma, panicle: orientation of branches other than grouping characteristics taken. Principal component analysis indicated that first eight components accounted for more than 90% of the total genetic variation. The characters contributing more positively with the PC1 and exhibited maximum variability among the varieties were leaf length, leaf: stem ratio, plant height and 1000-grain weight. Cluster analysis through D2 statistics sequestrated 21 varieties into 5 clusters during both the year and in pooled analysis. It would appear logical to affect crosses between genotypes belonging to the clusters separated by high estimated statistical distance. In pooled analysis maximum inter-cluster distance (99.60) between cluster II and V followed by 95.29 between cluster II and IV were found. From the cluster mean value of pooled analysis, the promising cluster identified for desirable characteristics were cluster IV for maximum plant height, cluster II for late time of panicle emergence, cluster III for high green fodder yield and dry matter yield, cluster II for late maturity and cluster V for early maturity. On the basis of present study, 33 morpho-physiological characteristics studied could be used for the development of DUS descriptors in oat. Results of PCA and D2 analysis indicated substantial variation among the varieties with respect to different characteristics which can be implicated in the oat breeding programmes for the development of improved cultivars.