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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    IN-VITRO STUDIES ON NANOPARTICLE MEDIATED BIODEGRADATION OF ELECTRONIC WASTE BY NATIVE BACTERIA
    (2023-02-01) Chakraborty, Moumita; Rai, J. P. N.
    The management of electronic waste (e-waste) becomes a global issue in this digital era. Plastics are neither the main residue nor the most important pollutant in electronic trash, but they take up a lot of room due to their low density and the forms of their parts. They make up about 17 percent of the WEEE (Waste from Electrical and Electronic Equipment) stream. More than 55% of all the polymers in e-waste are made up of ABS (Acrylonitrile Butadiene Styrene) and HIPS (High Impact Polystyrene Sheet). Existing conventional practices are harmful for dealing with e-waste, therefore, indigenous soil bacteria were explored for e-waste treatment through enrichment culture approach followed by screening, identification and their characterization used for in-vitro biodegradation investigation. Soil bacteria were enriched in the presence of e-waste for 6 months and the findings were established through optical density value that were higher in the case of soil enrichment than the control. Based on their morphological, biochemical and molecular characterizations, the bacterial isolates MGP1, MGP4 and MGP15 were identified as Bacillus aryabhattai, Sporosarcina sp., Rhodococcus kroppenstedtii. The selection and biocompatibility testing of potential isolates were performed for the formation of bacterial consortia. All the isolates displayed their best performance at pH 7, temperature 30°C and shaking speed 120 rpm. Supplementing the nutrient medium with additional carbon and nitrogen sources enhanced the rate of polymer degradation by the bacterial isolates. A known bacterial strain Pseudomonas fluorescens was selected for the degradation of the electronic waste and its comparison with that of screened bacterial isolates. Comparative studies showed that e-waste degradation potential of consortium is better than the individual bacterial strains. Titanium Dioxide (TiO2) nanoparticles were used to study its effect on biodegradation ability of the best suited bacterial strain. The biodegradation of e-waste by the selected strains during in vitro experiment was confirmed by analytical processes like FT-IR, FESEM, EDX elemental analysis. Thus, this study besides providing direct and standardized protocol for screening and selection of efficient e-waste utilizing bacteria is also demonstrating potential consortia which are ready to be used. The bacterial isolates were able to degrade e-polymer both in media and soil and therefore can be used profitably for field scale bioremediation technology.
  • ThesisItemOpen Access
    Biodegradation of Zenzo (α) Pyrene by native bacteria employing nanoparticles
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-02) Punetha, Arjita; Rai, J.P.N.
    In present study, potential of microorganisms to degrade a high molecular weight polyaromatic hydrocarbon viz. Benzo(α)Pyrene (BaP) has been investigated. Three bacteria isolated from coal tar contaminated and fire affected soils were tested for their BaP degrading ability in minimal medium and soil slurry. Based on their morphological, biochemical and molecular characterization, the bacterial isolates were identified as Cellulomaons sp. 4D, Commamonas sp.1B and Streptomyces sp. 2C. All the isolates performed best at pH 7, temperature 30° C and shaking speed 100 rpm. Supplementing the minimal medium with additional carbon and nitrogen sources enhanced the rate of BaP degradation by the bacterial isolates. Comparative studies showed that BaP degradation potential of consortium is better than individual bacteria. Viability of bacterial cells has also been tested by storing them in bioformulation made of charcoal and talc and in alginate beads for 60 days. BaP degradation was also investigated using bacterial isolates immobilized in sodium alginate beads and using nanoparticles in both minimal medium and soil. Results showed that the degradation potential of bacterial isolates increased when used in immobilized form. Nanoparticles enhanced the ability of bacterial isolates to degrade BaP in soil though much difference was not observed in minimal medium. HPLC and FTIR analysis confirmed the degradation of BaP by the selected bacterial isolates. The current study clearly demonstrated that Cellulomaons sp. 4D, Commamonas sp. 1B and Streptomyces sp.2C were able to degrade BaP effectively in media and soil and therefore can be used profitably for field scale bioremediation technology
  • ThesisItemOpen Access
    Decolourization and degradation of textile dyes (Orange G and Methylene blue) by immobilized and free state bacteria
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-02) Anamika Kumari; Rai, J.P.N.
    In the present research, ability of microorganisms to decolorize and degrade textile dyes has been investigated. Bacterial strains were isolated from wastewater released from dye industries and were tested for their dye degrading ability in minimal medium and nutrient both. Based on the cultural, morphological and molecular characterization, the bacteria were identified as Lysinibacillus Ek.IIPR, Bacillus thuringiensis N3, Bacillus muralis 1Y118. These bacteria and their mixed consortium were used for the treatment of different concentrations of azo dye Orange G and basic dye Methylene Blue. Comparative studies show that consortium was most efficient in dye degradation and was able to degrade upto 94.10 and 92.56% of Orange G and Methylene Blue respectively. Dye decolourization was also checked by these bacteria immobilized in alginate beads. The use of bacteria in immobilized static state showed better decolourization potential as compared to the freely suspended bacterial cells. Anaerobic condition is obtained under static condition which favors the breakdown of azo dyes. The favorable pH recorded was 7 and optimum temperature for dye degradation was 30 °C above which, the degrading rate slowed down. Comparative study investigating the Phytotoxicity of pure dye samples and bacterial treated samples was also conducted. The germination % observed after the treatment was 76.66 and 81.26 % respectively. In FTIR study, peak at 1622 and 1624 cm-1 in Orange G and Methylene Blue indicates presence of azo bond as chromogen in control sample. Whereas, FTIR spectra of bacterial consortium treated dye samples reveal shifting of band and formation of new peaks in bacterial treated Orange G and Methylene Blue dye solution, which indicates degradation of dye. Comparative study of HPLC chromatogram of Orange G and Methylene Blue was also done.
  • ThesisItemOpen Access
    Wastewater treatment and bioelectricity generation using constructed wetland integrated with microbial fuel cell
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-07) Narayan, Maitreyie; Srivastava, R.K.
    A microbial fuel cell coupled with constructed wetland (CW-MFC) is a new and innovative device used to treat the different types of wastewater and produce energy which has also more wastewater treatment efficiency and more easy maintenance than others MFCs. In this work, the effects of hydraulic residence time (HRT) and different wetland plants were used for treatment and on the electricity production. The degradation characteristics of different wastewater were also investigated. The degradation rate and the electricity production increased to a peak before slowing down with the elongation of HRT. The highest degradation rate was obtained when HRT was 5days, the concentration of pollutants such as BOD, COD, chloride, sodium, potassium, sulphate and total nitrogen were reduced to by 96.98, 87.83, 62.91, 72.23, 82.71, 86.32, 78.55% respectively and pH was 7.23 by Canna and 96.97, 85.06, 70.14, 70.31, 69.48, 79.75, 69.95% respectively by Reed and pH was 7.23. Highest voltage generation was recorded 31.66 mV by Canna and 25.33 mV by Reed in the setup-1 and almost similar trends were also observed with other setup of other experiment for voltage and current generation and pollutant removal. The artificial neural network (ANN) models were developed on a daily basis with sigmoid as an activation function and subjected to a maximum of 20 iterations. In the setup-1, among the various ANN models, 4-4-1 was consider best because in this model with highest r- value, similarly in the setup 2,3,4, and 5, 4-2-1, 4-8-1, 4-10-1 and 4-8-1 models were found most suitable. Immobilized peroxidase enxyme on sodium alginate beads was also made and used in the microcosm for lignin dedradation. Optimization of various concentrations of wastewater and HRT for degradation of the lignin, electricity generation and COD removal by peroxidase enzyme were also studied mathematically by using Response Surface Methodology model. After optimization the maximum values were calculated treatment - 74.99%, HRT- 3.08 days and concentration of peroxidase 10 mg/L.
  • ThesisItemOpen Access
    Estimation of above ground biomass and net primary productivity of Kumaon Himalayan forest using geospatial approach
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-02) Rabha, Amit Kumar; Srivastava, R.K.
    The present studywas conducted in four districts of Kumaon region namely Udham Singh Nagar, Nainital, Almora and Bageshwar districts, Uttarakhand considering different altitudinal gradient. The study area is good representation of increasing altitudinal gradient from Udham Singh Nagar (148 m MSL) to Bageshwar district (>4000 m MSL). The study area is classified into five major forest classes along altitudinal gradient based on composition of dominant tree species. The major classified forest classes are mixed deciduous forest, mixed pine forest, pine forest, mixed oak forest, high altitude mixed forest and occupied about 54%, 19%, 17%,4% and 6% of studied total forest area respectively. The distribution of major forest classes also analysed along increasing elevation. In the current study, the estimation of Above Ground Biomass(AGB) of major forest classes was calculated by allometric and remote sensing (RS) based method and developed a multi parameter AGB prediction model using Artificial Neural Network Backpropagation algorithm and the Net Primary Productivity (NPP) was calculated using a modified remote sensing micrometeorological model refers to as MODNPP for monthly, seasonaland annual basis. For seasonal NPP study, include four season namely winter, summer, monsoon and autumn season.The remote sensing based estimated biomass of mixed deciduous forest, mixed pine forest, pine forest, mixed oak forest and high altitude mixed forest was measured within ranges from 184 Mg ha-1 to 436 Mg ha-1, 62 Mg ha-1 to 252 Mg ha-1, 80 Mg ha-1 to 302 Mg ha-1, 71 Mg ha-1 to 265 Mg ha-1, and 43 Mg ha-1 to 195 Mg ha- 1 respectively. The monthly NPP was measured in the ranges from 0.2 to 2.80 ton ha-1month-1. The seasonal NPP was measured for different season between 0.12 to 2.36 ton ha-1season-1(winter), 0.98 to 5.33 ton ha-1season-1 (summer), 1.78 to 8.12 ton ha-1season-1 (monsoon) and 0.26 to 2.47 ton ha-1 season-1 (autumn). However, the annual NPP was recorded between 1.5 to 16.5 ton ha-1year-1 for different location. The calculated annual NPP for different major forest types namely mixed deciduous forest, mixed pine forest, pine forest, mixed oak forest and high altitude mixed forest ranges from 7 to 13.93 ton ha-1year-1, 3 to 14 ton ha-1year-1, 3 to 15. ton ha-1year-1, 4 to 15.48 ton ha-1 year-1 and 5 to 15.17 ton ha-1year-1 respectively. Therefore, this study envisaged that models used for calculation of AGB and NPP are best suited for its calculation and cost effective.
  • ThesisItemOpen Access
    Groundwater heavy metal pollution remediation using phycosynthetically produced iron and silver nanoparticles
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-01) Negi, Surindra; Singh, Vir
    Heavy metal (Pb, Cr, Ni, Zn, Fe, As, Cd and Cu) concentrations in groundwater from seven selected locations in and around the industrial city of Rudrapur in the Terai area of Uttarakhand during different seasons (pre-monsoon, monsoon and post-monsoon) were determined. Important physico-chemical water quality parameters were also assessed. The HPI value was found higher during premonsoon season followed by monsoon and post-monsoon seasons, indicating that groundwater of the study area is critically polluted and not fit for drinking purpose. In the current study green synthesis of iron and silver nanoparticles using aqueous extract of freshwater microalga, Tetradesmus sp., and their application in Pb and Cd remediation were also investigated. Morphological characterization of nanoparticles confirmed the synthesis of spherical nano iron and silver with average size of 4.75 and 15 nm respectively. FTIR confirmed the involvement of polysaccharide, protein and lipid biomolecules in the bioreduction as well as capping of nanoparticles. Batch study was carried out to determine the heavy metal removal efficiency of phycosynthesized iron and silver nanoparticles and adsorption parameters were optimized using univariate procedures. The adsorption parameters such as contact time, pH, adsorbate dose, adsorbent dose and temperature showed significant interaction (p<0.05) with metal ion and nanoparticles. The results showed 98-99 % removal of metal ions from synthetic as well as natural groundwater. No significant difference (p>0.05) in the removal efficiency of iron and silver nanoparticles was observed. Hence it can be concluded that both the nanoparticles are equally efficient in removal of heavy metals from water or wastewater. Thus, iron and silver nanoparticles with high reactivity and greater stability can be synthesized using green “nanofactories” like Tetradesmus sp.
  • ThesisItemOpen Access
    Biodegradation of s-triazine herbicide: Atrazine by native soil bacterial isolates employing nanoparticles
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-12) Hina Khatoon; Rai, J.P.N
    In present study a popular herbicide viz. atrazine degradation potential of two soil bacterial strains isolated from Crop research centre at G.B.P.U.A. & T., Pantnagar, Uttarakhand in year 2017 was investigated under optimized laboratory conditions. Based on cultural,morphological, biochemical and 16S rRNA gene sequencing, these bacterial isolates were identified as Bacillus badius ABP6 and Bacillus encimensis ABP8. All the isolates performed best at pH 7, 30ºC temperature and 150 rpm shaking speed. Supplementation of additional carbon and nitrogen sources in liquid medium enhanced degradation rate of atrazine by bacterial isolates. In an in vitro comparative biodegradation study conducted in minimal medium, soil slurry and soil microcosm, maximum degradation (84.80%, 86.40% and 87.70%) of atrazine was observed in treatment inoculated with bacterial consortium after 20 days. The atrazine degradation kinetics indicated that the degradation rate constant has ranged from 0.01 to 0.09 (minimal medium), 0.011 to 0.095 (soil slurry) and 0.012 to 0.138 (soil microcosm) for all the treatments. Immobilization of bacterial cells on sodium alginate beads, sugarcane baggase, nanozeolite and synthesized α-Fe2O3 nanoparticles showed maximum atrazine degradation in minimal medium, soil slurry and soil microcosm under consortium treatment, after 20 days as compare to free bacterial cells. SEM analysis revealed successful immobilization of bacterial cells on different immobilizing carriers. Rhizoremediation study conducted in maize (Zea mays) rhizosphere, further confirmed the siginificance of bacterial isolates in atrazine degradation. All bacterial treatments resulted in enhancement of maize growth response, chlorophyll and protein content, significantly over control. However carotenoid content was found to decrease in response to inoculation. Atrazine dissipation in soil and accumulation by Zea mays was observed in different time period and maximum accumulation was observed in roots while least was in leaves for all treatments. As compared to monoculture, consortium performed better in case of all the experiments conducted. HPLC, FTIR and GC-MS analysis confirmed the formation of metabolites as a result of biodegradation. Utilization of dynamic microorganisms is an efficient and economical strategy, while microbial immobilization gives a breakthrough in the restricted application of microbes in in-situ. The study clearly demonstrated that B. badius ABP6 and B. encimensis ABP8 were able to metabolize atrazine effectively and thus could be employed profitably for field scale bioremediation technology.
  • ThesisItemOpen Access
    Chlorpyrifos biodegradation and its process optimization employing Biphasic Bioslurry Bioreactor System (BBBS)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-01) Pant, Apourv; Rai, J.P.N
    Biodegradation study of organophosphate chlorpyrifos (CP) by two potential soil bacterial isolates was investigated under controlled laboratory conditions. The two strains, named as AP1 and AP2, based on cultural, morphological, biochemical and16s RNA profiling were identified as Pseudomonas aeruginosa and Bacillus pumilus, respectively. Initial concentration 200mg/l of CP was supplemented in minimal salt broth medium for checking the biodegradation potential at pH 6.5, 7.0, 7.5 and temperatures 25°C, 30° and 35°C. The CP biodegradation was slow, initially for first 5-10 days and later on accelerated. The CP degradation potential of Pseudomonas aeruginosa and Bacillus pumilus was strongly influenced by pH and temperature with maximum degradation at pH 7.0 and temperature 30°C followed by pH 7.5 and 35°C at the end of 20 days. Lowest CP degradation was observed at pH 6.5 and 25°C temperature. The optimal conditions for both isolates were pH 7.0 and 35°C temperature. At the end of 20 days 86.6% and 84.72% CP was degraded by Pseudomonas aeruginosa and Bacillus pumilus respectively at optimum pH of 7.0 and 30°C temperature. Further the study was elaborated by constructing a pilot scale biphasic bioslurry bioreactor (BBBS) and its optimization was done following Taguchi’s Design of Experiment Methodology (DOE). Six factors, which significantly influence the performance of the two phase bioslurry reactor were considered, viz., substrate-loading rate(SLR), slurry phase pH, DO of soil mixture, soil water ratio, operating temperature and soil microflora (as CFU). Among which DO of soil mixture was found to be the most important factor influencing the biodegradation process. The optimum operating conditions obtained from the methodology showed enhanced chlorpyrifos degradation from 283.86 μg/g to 955.364 μg/g by overall 70.34% of enhancement in biodegradation. This study clearly demonstrated that Pseudomonas aeruginosa AP1 and Bacillus pumilus AP2 were able to metabolize CP efficiently and thus could be employed in a sustainable way for field scale bioremediation of CP and alike chemicals.
  • ThesisItemOpen Access
    Development of ecofriendly hybrid model for wastewater treatment
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Solanki, Praveen; Srivastava, R.K.
    Present study envisaged about the phytoremediation of domestic wastewater using floating raft based ecofriendly hybrid model at GBPUA&T, Pantnagar campus premises. Domestic wastewater used in this experiment was slightly alkaline in nature with average pH value of 7.3 with the EC of 624.1 μS/cm. The average concentration of TS, TSS and TDS were found to be 767.62, 527.12 and 244.5 ppm, respectively. BOD and COD of wastewater were 64.75 and 277.25 ppm, respectively. Major nutrients i.e., nitrate nitrogen, ammonical nitrogen, total nitrogen, phosphate and potassium were 11.44, 12.73, 25.73, 11.03 and 11.5 ppm, respectively. While sodium was 25.67 ppm. Among different parameters TSS, BOD, COD, phosphate and nitrate nitrogen were found beyond their discharge permissible limits as per the EPA Rules 1986. Both Canna and Pistia were examined first at lab level experiment to standardize the system for phytoremediation of domestic wastewater in floating rafts and constructed wetland as well as validated the system at laboratory scale before field level study. Where both plants were showed good efficiency to remove various pollutants from the wastewater, moreover in the present experiment Canna plant was more superior as compared to Pistia. Among various models developed for wastewater treatment the hybrid system-1 (HS-1) which was combined with two floating raft tanks such as floating raft tank-I planted with Canna and floating raft tank-II planted with Water lily was found the most efficient and ecofriendly in nature for maximum reduction of various pollutants from the wastewater. After 48 h HRTs the remaining concentration of pollutants such as pH, EC, K, Na, TS, TDS, TSS, BOD, COD, PO4 -1, NH4 +-N, NO3 --N and TN were 7.1, 490, 3, 6, 262, 180, 82, 14, 80, 2.1, 3.18, 3.36 and 8.35 ppm, respectively with their percentage reduction by 5.33, 20.98, 71.43, 77.25, 66.00, 65.19, 67.65, 78.71, 71.56, 79.06, 76.84, 73.08 and 71.79 %, respectively. After this treatment those parameters were beyond their discharge permissible limits were reduced within the permissible limits. The trend of biomass production by Canna and Pistia was inversely proportional as the concentration of cadmium, chromium and lead was increased from 5 to 150 ppm. When the concentration of these heavy metals was beyond the 100 ppm both the plants died due to phytotoxicity. Maximum concentration of Cd, Cr and Pb such as 58.69, 35.61 and 67.77 ppm, respectively were found in root of Canna and 10.13, 6.22 and 15.74, respectively in shoot of Canna which were higher as compared to these metals concentration in the root and shoot of Pistia.