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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Evaluation of pyrolyzed pine needle bio-oil for antifungal and nematicidal activities with molecular docking analysis on target proteins
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-09) Chandra, Kailash; Verma, A. K.
    Pyrolyzed pine needle bio-oil formulation was used to evaluate antifungal and nematicidal activity. Antifungal activity of pine needle bio-oil (40%) based formulation and its four dilutions (10-1, 10-2, 10-3 and 10-4) were evaluated in both condition in-vitro and in-vivo. In-vitro activity of the formulation and its 10-1 showed 100% inhibitory activity against Alternaria brassicae while in 10-2, 10-3 and 10-4 dilutions the inhibitory activity was 52%, 48% and 42% respectively. The pre-conditioning brassica plants treated with the foliar spray of antifungal pine needle bio-oil formulation showed 100% inhibition of Alternaria brassicae while in post-conditioning plants treated with dilutions 10-1, 10-2, 10-3 and 10-4 were showed disease severity of about 3.3%, 6.6%, 13.3% and 30% respectively. Biochemical analysis of pine needle bio-oil formulation treated Brassica leaves revealed that the chlorophyll and carbohydrates content of post-conditioning plants increased with respect to the increase in bio-oil dilutions while protein content was found to be decreased. In case of post-conditioning plants, only protein content was increased while the chlorophyll and carbohydrates content was found to be similar. Molecular docking analysis of four compounds present in pine needle bio-oil, 4-methyl-pentanoic acid and nonanoic acid with succinate dehydrogenase while 1-(1H-Imidazol-4-YL)-Ethanone and dehydroabietic acid with lanosterol 14α demethylase of Alternaria brassicae. Showed dock score -4.03 and -6.9 Kcal/mol for the enzyme succinate dehydrogenase and -9.6 and -6.8 Kcal/mol for lanosterol 14α demethylase respectively. Nematicidal activity of pine needle bio-oil based formulation was analyzed against root knot nematode Meloidogyne spp. The concentration of formulation 5%, 7.5% and 10% showed maximum 100% inhibition against mortality, immobility and egg hatching of Meloidogyne spp. Molecular docking analysis of four compounds present in pine needle bio-oil, dehydroabietic acid and 2-methyl phenol with thymidylate synthase while dehydroabietic acid and 4-Methyl Pentanoic acid with Na-K ATPase of Meloidogyne spp. Showed dock score -7.1 and -4.94 Kcal/mol for thymidylate synthase and -5.0 and -4.8 Kcal/mol for Na-K ATPase respectively. Hence the pine needle bio-oil based formulation is more effective against Alternaria brassicae nematode Meloidogyne spp
  • ThesisItemOpen Access
    Biochemical appraisal of differentially treated Banana (Musa acuminata L.) cv. Grand Naine for post-harvest quality
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-09) Mathur, Ananya; Vandana A. Kumar
    The study conducted during 2022, in biochemistry department, cbsh, gbpuat, was aimed to enhance the shelf-life of banana (musa acuminata l.) cv. grand naine by applying aqueous solutions of salicylic acid (s: 1mm), gibberellic acid (g: 1mm), 1- methylcyclopropene (m1 to m5, ranging between 50μl/l to 800 μl/l) and salicylic- +gibberellic acids (sg1: 1mm each and sg2: 1mm of salicylic- and 2mm gibberellic acids) for 15 minutes followed by air drying and storage for 20 days at room temperature (24ºc- 27ºc). enzymatic studies, biochemical parameters, sensory evaluation and physical observations, in triplicates were performed at 5day-intervals. the increase in activities of cellulase, invertase, xylanase, polygalacturonase and peroxidase (0.071±0.006, 0.002±0.001, 0.0206±0.013, 0.004±0.0001, 0.030±0.001 u/100gfw, respectively, on day 20) viz-a-viz control (0.365±0.002, 0.0041±0.01, 1.7462±0.016, 0.014±0.001, 0.0520±0.001, respectively, on day 20) and contents of total soluble protein, soluble sugars and total phenols (0.308g/100g, 3.23%, 61.2mgae/100g, respectively, on day 20) was the least for m5 treatment and highest for s treatment. all mcp treatments delayed ripening as they performed better than s, g, sg1 and sg2, throughout storage. the decrease in total starch, resistant starch, total flavonoids, titratable acidity and ascorbic acid (72.73%, 53.9%, 15.5mqe/100g, 0.7%, 85±0.04mg/100ml, respectively, on day 20) was the least for m5 treatment. other treatments of mcp also decreased total starch, resistant starch and total flavonoid contents, concomitant to their increasing concentrations. the decrease in activities of cellulase, xylanase, polygalacturonase, soluble sugars and increase in contents of total starch, resistant starch and total flavonoids by sg1 & sg2 were significantly lower than those shown by s & g alone. delayed color and texture changes were observed in m5 treated banana which remained ‘hard’ (texture score: 1) and ‘yellow with green tips’ (color score: 2) till 10 days of storage. an enhanced shelf-life of banana was observed for 10-15 days when treated with 800 μl/l of mcp treatment and stored at room temperature.
  • ThesisItemOpen Access
    Biochemical evaluation of ethnomedicinal tree Oroxylum indicum (L.) Kurz
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-09) Pant, Samridhi; Dubey, Ashutosh
    Oroxylum indicum belongs to the monotypic genus Oroxylum. It has long been used in traditional medicinal systems and is an important part of Ayurveda. In the present research the antioxidant activity and the secondary metabolites’ constitution of seed and stem bark of O. indicum in the aqueous and hexane extracts was studied. Total antioxidants (12.792±0.1 mgAAE/g, 14.961±0.04 mgAAE/g), total phenolic (136.181±0.2 mgGAE/g, 135.08±0.15 mgGAE/g), total tannin (56.999±0.8 mgTAE/g, 26.833±0.4 mgTAE/g), total flavonoid (88.911±0.41 mgQE/g, 122.925±0.30 mgQE/g) and total flavonol content (25.832±0.23 mgCE/g, 27.885±0.087 mgCE/g) was higher in aqueous extract for both seed and stem bark than hexane extract, respectively. Total antioxidants showed positive correlation with the phytochemicals. The metal ion chelating activity (IC50) (32.404±0.14 μg/mL and 30.756 ±0.27 μg/mL), DPPH scavenging (IC50) (101.396±0.8 μg/mL and 115.358±0.4 μg/mL) and reducing power (RP50) (37.115±0.02 μg/mL and 40.134±0.5 μg/mL) was also higher in the aqueous extract of seed and stem bark than the hexane extracts as expressed by their lower IC50 values respectively. All activities showed a negative correlation with the phytochemicals indicating the increase in the phytochemicals will lead to better antioxidant potential by lowering the IC50. The extracts were tested for their anti-inflammatory activity. The results were expressed as IC50 values which were 82.26±0.5 μg/mL and 79.681±0.4 μg/mL for the aqueous extract which was better than the hexane extract. Anti-bacterial activity of the extracts was tested against Xanthomonas oryzae and activity was observed only in the aqueous extract of seeds. A maximum zone of inhibition of 18 mm was observed at 5000 μg/mL. The seed proteins of O. indicum were fractionated and tested for their amylase and protease activity and inhibition. Highest amylase activity was observed in albumins and highest inhibition was observed in the prolamin. For the protease activity, albumins and globulins showed almost same protease activity but the highest inhibition was observed in globulins. The seed proteins of O. indicum seeds were partially purified using ammonium sulphate precipitation and dialysis and characterized using SDS-PAGE. The process showed 9 bands on the gel with molecular weight ranging from 97 kDa to 14.3 kDa.
  • ThesisItemOpen Access
    Studies on clonal diversity in eucalyptus using biochemical and molecular approaches
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-09) Belamana, Bhargavi; Dubey, Ashutosh
    Eucalyptus is a fast growing evergreen tree with its rapid growth and capacity to satisfy rural needs for fuel and shelter, its plantations were widely encouraged. The current work details about the evaluation of the clonal diversity through biochemical and molecular parameters among five Eucalyptus clones acquired from Agro Forestry Research Centre, GBPUAT, Pantnagar. Total phenol content was observed to be lowest (109.6±0.05 mg GAE/g) in sample PES-21-4 and highest (132±0.09 mg GAE/g) in sample K 25. Total antioxidant capacity was observed to be lowest (15.22±0.006 mg/g) in sample PES-21-3 and highest (17.89±0.02 mg/g) in sample PES-21-2. Total flavonoid content was observed to be lowest (19.48±0.2 mg CE/g) in sample PES-21-3 and highest (24.81±0.3 mg CE/g) in sample PES-21-1. Total tannin content was observed to be lowest (9.12±0.05 TAE/g) in sample PES- 21-1 and highest (10.17±0.13 TAE/g) in sample PES-21-4. IC50 value of DPPH activity was observed to be lowest (23.49±0.009 μg/ml) in sample PES-21-3 and highest (26.01±0.005 μg/ml) in sample K25. IC50 value of metal ion chelating activity was observed to be lowest (17.76±0.11 μg/ml) in sample PES-21-3 and highest (19.86±0.03 μg/ml) in sample K25. Amylase inhibition was found to be highest in hexane extract (46.04±0.11%) than the methanolic extract (34.59±0.03 %). SDS-PAGE analysis revealed 15 bands out of which the most prominent band obtained was band 14 of size 92.7kDa with Rf value of 0.277. Five Eucalyptus clones were characterized at molecular level using eight RAPD primers. The minimum similarity coefficient was between clone PES-21-1 and clone PES-21-4 (0.63), while maximum similarity coefficient was between clone PES-21-3 and clone PES-21-4 (0.79). Dendogram revealed that Clone PES-21-3 and clone PES-21-4 are showing high similarity while Clone K 25 was quite diverse from rest of the clones. The quantitative determination of the phytochemical composition and their activities in Eucalyptus helps to understand its therapeutic properties and utilizing molecular markers to determine the clonal diversity leads to a better knowledge about plant evolutionary history and provide valuable information about agrobiodiversity dynamics.
  • ThesisItemOpen Access
    Investigation on phytochemical attributes and antioxidative potential of Pleurotus sajor-caju (Fr.) Singer
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-09) Srinivas, Arji Ravi Chandra; Punetha, Himanshu
    The current work details about the evaluation of the phytochemical composition of different polarity extracts and the biological activities of mushroom P.sajor-caju sample acquired from mushroom research and training centre, G. B. Pant University of Agriculture & Technology, Pantnagar. Twenty one compounds were identified with the help of GC-MS representing 98.61% of total methanolic extract composition. Cis-Vaccenic acid, Beta-Monolinolein, ergosterol derivatives are major compounds identified. Fifty two compounds were identified with the help of GC-MS representing 99.48 % of total petroleum ether extract composition. Linoleic acid, Eicosane, Heneicosane and Hexatriacontane were the major compounds identified. The Methanolic extract showed potent antioxidant activity in terms of DPPH radical scavenging, metal chelating activity and reducing power activity having IC50 value of 677.73±1.72μg/mL, 4.25±0.05 mg/mL and 9.66±0.11 mg/mL respectively in comparison to petroleum ether extract which might be due to presence of Cis-Vaccenic acid, Beta-monolinolein. Total phenolic content (TPC), Total flavonoid content (TFC) and Total antioxidant content (TAC) of methanol and petroleum ether extract were also evaluated. TPC, TFC and TAC were higher in methanol extract having 21.3±0.006 mg/gm, 3.43±0.002 mg/gm, and 19.8±0.65 mg/gm, respectively. Crude protein content was observed as 19.78% along with mineral composition Fe, Mn, Cu and Zn as 0.0156±0.89 mg/g, 0.029±1.56 mg/g, 0.018±1.25 mg/g and 0.085±1.23 mg/g respectively. The molecular docking of bioactive compounds Ergosterol and β-glucans of P sajor-caju against Covid-19 spike proteins 6vsb, 6lxt, 6lvn and 6vw1 was performed using PyRx software by autodock wizard as the engine for docking. The results obtained shows binding energies of 6vsb, 6lxt, 6lvn and 6vw1 with ergosterol as -9.3 kcal/mol, -7.9 kcal/mol, -7.1 kcal/mol and -8.7 kcal/mol respectively. The binding energies of 6vsb, 6lxt, 6lvn and 6vw1 with β-glucans as -6.8 kcal/mol, -7.5 kcal/mol, -6.2 kcal/mol and -7.5 kcal/mol respectively. The mushroom P. sajor-caju thus have potent medicinal properties and if explored further might be potent antioxidant and antiviral cost-effective agent compared to synthetically derived agents from pharma industries.
  • ThesisItemOpen Access
    Effect of gamma Iiradiation on quality of wheat and buckwheat flour
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2022-09) Mehra, Dakshita; Agrawal, Sanjeev
    Cereals and pseudocereals constitute a large part of human diet. The quality of flour during storage gets deteriorated by change in the temperature conditions, moisture, fungus development, pest infestations and many other variables. Gamma rays have been used to avoid food deterioration. The present study was undertaken to evaluate the quality of flour after exposing it at different dose of gamma radiation. The effect of gamma irradiation doses (1, 2, 5, 7 and 10 kGy) on quality of wheat and buckwheat flour was studied. The nutritional, antioxidant and antinutritional parameters like crude protein content, total starch content, amylose, amylopectin content, amylose to amylopectin ratio, resistant starch, total phenol, flavonoid, total antioxidant content, tannin and phytic acid content in non irradiated wheat varieties ranged from 11.15%-13.14% , 61.39-67.08 gm/100g, 17.62-22.62 gm/100g, 82.38-77.38 gm/100g, 0.21-0.29, 0.50-0.74 gm/100g , 1.19-1.38mg GAE/g, 0.22-0.36 mg QE/g, 12.44-16.22 μmole of vit C/gm, 8.48-6.03 mg TAE/g and 280.48-253.65 mg/100g, respectively whereas in buckwheat it was found to be 11.22%, 71.41 gm/100g, 24.62 gm/100g, 75.38 gm/100g, 0.32, 0.85 gm/100g, 4.41 mg GAE/g, 3.92 mg QE/g, 19.65 μmole of vit C/gm, 14.84 mg TAE/g and 235.47 mg/100g, respectively. After exposure to gamma radiations, no significant difference was observed in crude protein and total starch content, whereas amylose, resistant starch, phenol, flavonoid and total antioxidant content were increased significantly (p≤0.05) in both wheat and buckwheat with increasing doses. Tannin, phytic acid and amylopectin content were found to decrease on exposure of irradiation. A positive high correlation was observed between total phenol, flavonoid and antioxidant content in both irradiated wheat and buckwheat. It may be inferred that gamma irradiation doses does not deteriorate the quality of wheat or buckwheat flour however, it may improve the quality by decreasing the antinutritional contents and increasing the antioxidants.
  • ThesisItemOpen Access
    Biological activities of extracts and essential oils of Anaphalis margaritacea- (L.) Benth. & Hook.f. in relation to its phytochemical composition from hilly terrains of Uttarakhand Himalaya
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2022-09) Arti; Dubey, S.K.
    Present research details the phytochemical composition of essential oils, as well as biological activity of the plant Anaphalis margaritacea in different polarity extract, collected fromKumaun region of Uttarakhand at an elevation of 2,200m. Total 67compounds were revealed in essential oil via GC-MS, however 8 remained unidentified, comprising 94.04% of the total essential oil composition. The major compounds in the essential oil comprised of Caryophyllene (E) (17.82%), Asarone (8.89%), humulene (8.27%), and caryophyllene oxide (7.00%). Thirty four compounds were identified constituting 89.09% of the total methanolic extract which include 5R, 8R, 9S, 10R)-2-Formyl-3-Hydroxy-5-Isopropeny-8-8Methyl (3a10)- octahydronaptho (13.15%), oleic acid (11.31%) etc, Identified compounds represented 70.21% of the total hexane extract composition with prominent compounds Hexatriacontane (20.43%), Tetracontane (14.70%), Heneicosane (2.66%), and Tetratetracontane (2.05%). Total phenolic content (TPC), Total flavonoid content (TFC) and Total antioxidant content (TAC) of methanol and hexane extract were also evaluated. TPC, TFC and TAC were higher in methanol extract having 19.11±0.24, 34.07±1.54, and 28.09±1.82 (mg/gm), respectively. DPPH radical scavenging was expressed as IC50 values which were 75.94 ±0.66 μg/mL in methanolic extract, 141.42 ± 0.73μg/mL in hexane extract and 57.66 ± 0.40 μg/mL in standard ascorbic acid. For metal chelating activity, the IC50 value in methanolic extract, hexane extract and standard EDTA was 72.90±0.096, 141.42±1.25, 60.18± 0.39 (μg/mL) respectively. For reducing power activity, the RP50 value of gallic acid, methanolic and hexane extract were 74.37±0.29 μg/mL, 79.64±5.36 μg/mL, 107.0682±1.34 μg/mL respectively. Methanolic and hexane extracts exhibited potent anti-inflammatory activity with IC50 values of 37.58±1.05 μg/mL, 46.92±0.67 μg/ mL respectively. Diclofenac sodium salt used as standard (IC50 value 51.59±1.18 μg/mL). The results suggest that A. margaritacea have potent biological activities and can be a powerful antioxidant and anti-inflammatory agent if explored further. The plant extracts might be more affordable than synthetically generated compounds, eco-friendly, and economically viable for the pharmaceutical and biomedical industries.
  • ThesisItemOpen Access
    Biological activities of essential oil and whole plant extracts of Callicarpa macrophylla Vahl. in relation to its phytochemical composition
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2022-08) Arya, Aradhana; Arya, Aradhana; Dubey, S.K.; Dubey, S.K.
    The present study gives a detailed explanation of the phytochemical profile of essential oil and different polarity extracts along with the evaluation of the biological activities of the plant Callicarpa macrophylla collected from the hilly regions of Uttarakhand from the elevation of 1394 m. Fourty-five compounds were identified by GC-MS accounting for 78.79% of the total essential oil composition in which β- Selinene (15.24%) was the prominent constituent. The methanolic extract of the whole plant comprised of 26 compounds constituting 79.74% of the total methanolic extract composition. The major compounds in methanolic extract were Trachylobane (31.87%) and Palmitoleic acid (8.26%). Thirty-two compounds were identified in the hexane extract comprising 63.56% of the total composition. The prominent compounds in hexane extract were Tetratetracontane (9.78%) and Pentatriacontane (7.15%). The Total phenolic content (TPC), Total flavonoid content (TFC) and Total antioxidant content (TAC) was estimated in higher quantities in methanolic extract having 51.27±0.61 mg/gm, 49.47±0.34 mg/gm and 27.89±0.24 mg/gm, respectively; as compared to that of hexane extract having 40.58±0.50 mg/gm, 34.42±0.27 mg/gm and 11.42±0.23 mg/gm, respectively. Methanolic extract demonstrated potent antioxidant activity in terms of DPPH radical scavenging and reducing power activity having the IC50 and RP50 values of 91.97±0.33 μg/mL and 56.72±0.67 μg/mL compared to standards Ascorbic acid (57.66±0.40 μg/mL) and Gallic acid (74.36±0.29 μg/mL) respectively. Both methanolic and hexane extract showed better metal chelating activity; with IC50 value of 10.90±1.40 μg/mL and 41.60±1.31 μg/mL, respectively as compared to the standard EDTA 60.18±0.39 μg/mL. The methanolic extract displayed potent anti-inflammatory activity with an IB50 value of 64.09±1.31 μg/mL comparable to that of the standard Diclofenac Sodium (51.69±0.99 μg/mL). Callicarpa macrophylla possesses a strong therapeutic potential and, if explored further, may prove to be a powerful antioxidant, anti-inflammatory, and cost-effective agent compared to synthetically derived and can replace various artificial synthetic agents from pharmaceutical industries.
  • ThesisItemOpen Access
    Biochemical, phytochemical and in silico evaluation for medicinal properties of Eucalyptus tereticornis
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2022-01) Sharma, Pragya; Dubey, Ashutosh
    Eucalyptus is a fast growing evergreen tree, grown widely in various parts of India for its agronomical and medicinal uses. Its health benefits are due to its antioxidant properties. It is a reservoir of phytochemicals, which contribute to its antioxidant properties. In the current research work, the antioxidant activity of green leaves of Eucalyptus in methanolic and hexane extract, along with content of some phytochemicals (phenols, flavonoids, flavonols, proanthocyanidins and tannins) were determined. In methanolic extract, total antioxidant capacity was 17.23±0.09 mg/g, total phenolic content was 92.7±0.03 mg GAE/g, total flavonoid content was 53.7±0.10 mg CE/g, total flavonol content was 2.30±0.02 mg CE/g, tannin content was 9.23±0.01 mg TAE/g and proanthocyanidin content was 0.57±0.021 mg CE/g. The IC50 values for DPPH, FRAP and metal chelating activities were found to be 15.80±0.12 μg/ml, 8.41±0.03 μg/ml, 19.92±0.009 μg/ml, respectively. While in hexane extract, total antioxidant capacity was 8.25±0.06 mg/g, total phenolic content was 11.62±0.009mg GAE/g, total flavonoid content was 7.83±0.091 mg CE/g, total flavonol content was 0.89±0.001 mg CE/g, tannin content was 30.21±0.001 mg TAE/g and proanthocyanidin content was 35.13±0.07 mg CE/g. The IC50 values for DPPH, FRAP and metal chelating activities were found to be 28.81±0.001 μg/ml, 20.93±0.01 μg/ml, 26.44±0.04 μg/ml, respectively. All these parameters showed positive correlation with antioxidant activity, showing that increase in concentration of these compounds lead to increase in antioxidant activity of the plant. The hexane and methanolic extracts of green leaves of Eucalyptus was also tested for its antibacterial effect on gram positive S. aureus and gram negative E. coli. The hexane extract of green leaves of Eucalyptus showed better antibacterial activity on S. aureus than on E. coli with maximum zone of inhibition of 24 mm at 100μg/ml followed by minimum zone of inhibition of 6 mm at 25μg/ml respectively. However, methanolic extract did not show any antibacterial activity. The water soluble proteins from green leaves of Eucalyptus were partially extracted using SDS-PAGE. The process showed 13 bands out of which the most prominent band was obtained of size 14.3 kDa with Rf value 0.831. 1, 8- cineole, found in Eucalyptus oil has variety of medicinal properties, including anti- inflammatory and analgesic activities, which were analysed in silico. The binding energy of 1, 8- cineole with respective proteins (Trpa1 and Trpa2 for analgesic activity; Cox1 and Cox2 for anti- inflammatory activity) was found to be more negative when compared with that of synthetic drugs (Acetaminophen and Ibuprofen). Hence, it can be concluded that 1, 8- cineole can prove to be a better analgesic and anti- inflammatory drug. Therefore, it can be concluded that Eucalyptus is a pharmaceutically important plant whose medicinal potential needs to be explored.