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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Studies of bactericidal activity in pyrolyzed bio-oil against plant pathogenic bacteria
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-12) Baghel, Megha; Verma, A.K.
    Pyrolyzed bio-oils from pine needle (Pinus roxburghii), corn cob (Zea mays) Bambusa balcooa and Dendrocalamus hemiltonii was used to evaluate the antibacterial activity of different plant pathogenic and non-pathogenic bacteria Xanthomonas axonopodis, Xanthomonas campestris, Xanthomonas oryzae, Ralstonia solanacearum,and Pseudomonas fluorescens. The pine needle and corn cob bio-oil have approximated 50% content phenolic compounds. The bio-oil from these plant residues were prepared by pyrolysis. Three different formulations were prepared by adding (30%,40% and 50%) bio-oil, ethanol as solvent, saponin as surfactant and aqueous phase of bio-oil was used to make up the volume. The pine needles bio-oil formulations showed maximum zone of inhibition 18.49±0.092mm against Xanthomonas axonopodis at formulation F2. The in-corn cobbased formulation F2 showed 17.6±0.353mm zone of inhibition against Xanthomonas oryzae. Hence pine needle and corn cob-based formulation F2 was found effective for the antibacterial activity. The nano-emulsion of these formulation F2 were prepared after dilution up to 10-4 times and characterization by DLS and FTIR. The nanoparticles were homogeneously mono dispersed in the sonicated pine needle bio-oil formulation (dilution10-1) sample, that had a core diameter of 237.5 nm. While the corn cob-based formulation F2 had core diameter 667.3 mm. The FTIR data of nano-emulsion of both formulations were showed a characteristics difference after sonication. The bactericidal mechanism was identified by membrane permeability and membrane integrity test. The integrity and cell membrane permeability of pine needle bio-oil based formulation was found to be higher in case of Xanthomonas axonopodis, while that of corn cob bio-oil based formulation was found to be higher against Xanthomonas oryzae. These formulations were tested in field on Xanthomonas axonopodis infested soyabean plants. After 20 days of inoculation of Xanthomonas axonopodis soyabean plant the formulation F2 of pine needle-based formulation and corn cob-based formulation was sprayed on infested plant. It had been found that due to the slightly acidic nature of formulation the leaves were inflamed.
  • ThesisItemOpen Access
    Phytochemical screening for elite selection of foxtail millet (Setaria italica L.) from local cultivars of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-11) Tamta, Pawanesh; Verma, K.C.
    Foxtail millet is one of the oldest cultivated crop in the world. It belongs to the Setaria genus of the Poaceae family. Foxtail millet grains are rich in valuable phytochemicals such as essential amino acids, phenols and flavonoids etc. The nutritional benefits of Foxtail millet make it a crop worth exploring to improve its production and yield. The present study includes the screening of various phytochemical constituents present in the seven samples of Foxtail millet collected from local cultivars. Sample from Kasar Devi (Almora) had the highest moisture as well the oil content i.e. 9.59±0.01% and 5.90±0.06% respectively. Defatted seed samples showed higher protein content than the fatted seed samples. The highest protein content, both in fatted and defatted seed was found in the sample from Kasar Devi (Almora) i.e 11.60±0.44 g/100g and 13.35±0.44 g/100g respectively. The amino acid composition i.e. the highest content of tryptophan, lysine and arginine were found in the sample from Kasar Devi (Almora) i.e 2.77±0.03 g/100g protein, Masmoli (Pithoragarh) i.e 1.34±0.03 g/100g protein and Kasar Devi (Almora) i.e 3.21±0.03 g/100g protein respectively. The minerals (Calcium, Zinc, Manganese and Iron) showed the highest content in the sample from Kasar Devi (Almora) i.e 29.5 mg/100g, Dhauladevi (Almora) i.e 9.00 mg/100g, Masmoli (Pithoragarh) i.e 10.69 mg/100g and Kasar Devi (Almora) i.e 8.62 mg/100g of sample respectively. The highest antioxidant activity was found in the sample from Dhauladevi (Almora) i.e 74.75±0.23 mg/100g.The samples were studied quantitatively for total phenol content (TPC) and flavonoid content (TFC) in methanol and petroleum ether extracts. Methanolic extract exhibited higher total phenol and flavonoid content than the petroleum ether extract. In methanolic extract, the highest TPC was found in the sample from Chaura (Almora) i.e 14.30±0.04 mgGAE/100g, whereas, the highest TFC was found in the sample from Kasar Devi (Almora) i.e 11.33±0.05 mgQE/100g. In petroleum ether extract, the highest TPC was found in sample from Dhauladevi (Almora) i.e 0.55±0.04 mgGAE/100g, whereas, the highest TFC was found in sample from Kasar Devi (Almora) i.e 0.34±0.04 mgQE/100g. Anti-nutritional parameters were also estimated. Tannin and nitrate content were quantified in methanol and petroleum ether extracts. In methanolic extract, the lowest tannin content was found in the sample from Masmoli (Pithoragarh) i.e 2.07±0.02 mgTAE/100g, whereas, the lowest nitrate content was found in the sample from Kosi (Almora) i.e 25.35±0.95 mg/100g. In petroleum ether extract, the lowest tannin content was found in the sample from Kasar Devi (Almora) i.e 1.05±0.03 mgTAE/100g, whereas, the lowest nitrate content was found in sample from Kosi (Almora) i.e 8.77±0.07 mg/100g. The lowest oxalate content was found in the sample from Masmoli (Pithoragarh) i.e 24.55±0.04 mg/100g and the lowest phytic acid content was found in the sample from Pauri (Pauri Garhwal) i.e 0.47±0.04 mg/100g. Among all the samples, Kasar Devi (Almora) was found to be the superior in most of the parameters.
  • ThesisItemOpen Access
    Phytochemical analysis and evaluation of biological activities of Artemisia nilagirica (C.B. Clarke) Pamp. growing in Kumaon region of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-10) Garia, Vaishali; Dubey, S.K.
    The aim of current work is the evaluation of the phytochemical composition of essential oil along with different polarity extracts and the biological activities of plant Artemisia nilagirica collected from Jeolikote Uttarakhand at elevation of 1320 m. Essential oil from whole plant was extracted by hydrodistillation method. The GC-MS analysis of essential oil showed the presence of thirty-five compounds with majority of sesquiterpenes. Bromocyclohexane (18.22%) was the most abundant compound followed by Germacrene D (12.73%), Caryophyllene (8.31%), Eucalyptol (6.19%), and many more. Twenty-six compounds were identified in hexane extract of A. nilagirica comprising of total 73.30% area. The major compounds were tetracontane (15.21%), heneicosane (6.52%), phytyltetradecanoate (5.11%), tetratetracontane (4.96%). Twenty-seven compounds were identified in the methanolic extract contributing to 92.56% of total compounds identified. The major compounds were palmitic acid (13.25%), alpha-linoleic acid (10.32%), oleamide (9.41%), phytol (8.58%), muco-inositol (7.27%), neophytadiene (5.05). Essential oil showed high antioxidant activity in terms of DPPH radical scavenging with an IC50 value of 3.15±0.01 μg/mL compared to standard ascorbic acid with IC50 value of 2.54±0.00 μg/mL, respectively. Hexane and methanolic extract showed comparable metal chelating activity with standard EDTA having IC50 value of 2.23±0.01 μg/mL,2.41±0.02 μg/mL and 2.22±0.01 μg/mL respectively. Methanolic extract showed good anti-inflammatory properties compared to standard diclofenac having IC50 value of 2.97±0.03 μg/mL and 3.79±0.01 μg/mL respectively. The anti-inflammatory effect of methanolic extract may be due to the presence of phytol (8.58%) and neophytadiene (5.05%) which are present in high amount and reported to have anti-inflammatory properties. Total phenolic content (TPC), Total flavonoid content (TFC) and Total antioxidant content (TAC) of the extracts were also evaluated. TPC, TFC and TAC content was higher in methanol extract having 92.07±0.111 mg/g, 11.92±0.010 mg/g and 5.71±0.006 mg/g respectively. From the present thesis work it can be concluded that Artemisia nilagirica plant extracts and essential oil posses potent antioxidant and anti-inflammatory properties. The effective biological compounds of plant extracts like phenols, flavonoids can be potential alternatives to traditional medications.
  • ThesisItemOpen Access
    Utilization of waste plant tissue culture medium for the growth of plant growth promoting rhizobacteria
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-11) Bhattacharjee, Tamalika; Kumar, Vandana A.
    Present study was conducted during 2021 in the Department of Biochemistry, College of Basic Sciences and Humanities, GBPUAT for reutilization of waste PTC medium for growth of bacteria. The sample under investigation included three types of waste MS medium (S2,S3,S4) that had been discarded after sub-culturing of Fragaria ananassa, Nicotiana tobacum and Rheum emodii plantlets. S1 was the freshly prepared MS medium. The carbohydrate, glycine, vitamins ,N, P, S, Ca, Mg and K contents in all samples were analyzed spetrophotometrically, turbimatrically and by AAS techniques. Results depicted that in the used samples S2,S3,S4 sucrose and glycine reduced to 30-40%,inorganic nutrients and vitamins reduced to 8-20% as compared to S1 hence, data for all parameters analyzed in S2,S3,S4 was significantly different from S1. The waste MS medium in different combination, with nutrient agar (NA-MS-U 100:0 as control, NA-MS-U 50:50 and NA-MS-U 0:100 as test material) were tested for B1,B2,B3,B4,B5. The highest no of B2 colonies (67) developed in 0:100 at pH6 and followed by 59 AT pH 7 for B1. Gentiana kurro rhizosphere population of bacteria developed to 38 x 105 CFU at pH6, in NA-MS-U 50:50 followed by 33 x 105 CFU at pH 6, in NA-MS-U 50:50. The six isolates were obtained from NA-MS-U 0:100 medium which were rod shaped, 5 being gram –Ve and 1 being gram +Ve. One of the isolates named as I3 was positive for phosphate solubilisation activity, zinc solubilisation activity and IAA production test but –Ve for siderophore production. Hence, the waste MS medium could be used for growth of B2and could also be used for the growth of Gentiana kurro rhizospheric isolates.
  • ThesisItemOpen Access
    Biochemical investigation for functionally active principles of quality Brassica genotypes and profiling of seed storage protein
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-10) Pujari, Rohit; Punetha, Himanshu
    The family Brassicaceae possess different range of varieties that finds importance in medicine, agriculture, nutraceuticals, etc. Its health promoting factors including essential amino acids, β-carotene, flavonoids and other phytoconstituents has tremendous impact on health and hence can be used as a viable food ingredient. It possess some antinutrients like glucosinolates and phytic acid that restricts its use for human consumption. The present study includes the investigation of various constituents in the fifteen samples of various Brassica genotypes and the profiling of seed storage protein through SDSPAGE. The total antioxidant was recorded highest to be in sample Biochem2020-12 (22.092±0.0122 mg/g) while minimum was recorded to be in Biochem2020-6 (12.09±0.048), the highest content of tryptophan was observed in sample Biochem2020-04 (1.257±0.014 g/100g protein) while the minimum was observed to be in Biochem2020-03 (0.749±0.019 g/100g protein), the highest value of methionine was observed to be in sample Biochem2020-05 (2.177±0.011 g/100g protein) while the minimum was observed to be in Biochem2020-3 (1.852±0.038g/100g of protein) , the highest TPC was recorded to be in sample Biochem2020-15 (5.467±0.067 mg/g) while the minimum was recorded to be Biochem2020-6 (2.686±0.037 mg/g) , the ortho-dihydric phenol content was recorded to be highest in sample Biochem2020-08 (0.546±0.002 mg/g) while the minimum was recorded to be in Biochem2020-03 (0.287±0.001 mg/g), the total protein content observed among all the genotypes was recorded to be highest in Biochem2020-03 (34.77%) whereas lowest was recorded in Biochem2020-04 (31.51%). The highest oil content was observed to be in Biochem2020-12 (44.58%) while the lowest was observed in Biochem2020-09 (35.75%). The glucosinolate content was observed to be highest in sample Biochem2020-13 (81.23±1.002 μmol/g) while the minimum was observed in sample Biochem2020-03 (14.08±0.122 μmol/g). Biochem2020-6 proved to be a better anti-inflammatory agent having IC50 of 35.770±6.555μg/ml. The DPPH activity was best shown by sample Biochem2020-08 having percentage inhibition of 52.987±0.462 %. The reducing power of sample Biochem2020-04 having IC50 57.443 ±0.070 μg/ml was observed to be better among all the genotypes under investigation. The results were recorded with respect to quality check, PDZ-1. The protein profiling through SDS-PAGE resulted in the band pattern ranging from 14.3kDa to 40kDa.
  • ThesisItemOpen Access
    Phytochemical analysis and evaluation of biological activities of Anaphalis busua (Buch.-Ham.) DC. collected from Kumaon region of Uttarakhand
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-10) Bahuguna, Ananya; Dubey, S.K.
    The current work details about the evaluation of the phytochemical composition of essential oil along with different polarity extracts and the biological activities of plant Anaphalis busua collected from hilly areas of Bhowali Uttarakhand at elevation of 1654 m. The essential oil was chemically analysed by GC-MS for its phytochemical composition. Thirty-four compounds were identified and 3 were unidentified by GC-MS comprising 94.75% of total essential oil composition. Isocaryophyllene (14.07%) was the prominent compound of the essential oil and other major components identified were Pogostol (8.21%), δ- Cadinene (6.81 %), and γ- Curcumene (6.09 %). The essential oil shows significant DPPH free radical scavenging activity having IC50 value of 14.286±0.43μL as compared to standard Ascorbic acid which might be due to the presence of isocaryophyllene. Thirty-one compounds were identified constituting 94.95% of total methanolic extract. Mome inositol (31.03%), Vaccenic acid (10.80%) and Palmitic acid (9.82%) were the major compounds. Twenty-two compounds were identified comprising 68.24% of total hexane extract. Tetracontane (19.33%), tetratriacontane (14.14%) and heneicosane (5.10%) were present in prominent amount. Methanolic extract showed potent antioxidant activity in terms of DPPH radical scavenging and metal chelating activity having IC50 value of 81.71±1.334 μg/mL and 11.26±0.005 μg/mL respectively which might be due to presence of mome inositol. Methanolic extract also exhibited potent anti-inflammatory activity with IC50 value of 24.10±0.09 μg/mL whereas Diclofenac potassium salt used as standard had IC50 value of 18.95±0.03 μg/mL. Total phenolic content (TPC), Total flavonoid content (TFC) and Total antioxidant content (TAC) of methanol and hexane extract were also evaluated. TPC, TFC and TAC was higher in methanol extract having 94.86±0.005 mg/gm, 11.11±0.002 mg/gm, and 36.00±0.011 mg/gm, respectively. A. busua thus have potent medicinal properties and if explored further might be potent antioxidant and anti-inflammatory cost-effective agent compared to synthetically derived agents from pharma industries. Furthermore, the locally grown weed can also be used for its anti-inflammatory properties, as it has demonstrated effective anti-inflammatory activities compared to standard Diclofenac which is a marketed drug. Plant extracts and essential oil could be utilised as powerful antioxidants that might be environment friendly and economically cost viable for the pharmaceutical and cosmetic sectors as plant is found as weed in abundance in hilly areas.
  • ThesisItemOpen Access
    In vitro establishment, biochemical profiling and cytotoxic evaluation in critically endangered herb Nardostachys jatamansi
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-02) Gupta, Palak; Kumar, Vandana A.
    The medicinal herb Nardostachys jatamansi belonging to family Caprifoliaceae is a native of Himalayan region and grows at altitudes of 3300-5000 m asl. The growth of rhizome explant after 40 days of establishment in Woody Plant Medium supplemented with TDZ & GA3 (1 ppm & 0.5 ppm, respectively) showed 4.4 cm shoot length and 10.33 number of leaves. The roots& leaves collected in Oct-Nov (S-1), Jan-Feb (S-2) and June-July (S-3) from natural habitat and extracted in four different solvents viz. methanolic, ethylacetate, acetone and water were screened for phytochemical content and antioxidant activity. The roots of S-3 in methanolic extract showed the highest phytochemical contents like total phenol (63.76mg GAE g-1 extract), total tannin (204.67mg TAE g-1 extract) and ortho-dihydroxy phenol (18.8mg CE g-1 extract) whereas total flavonoid (48.5mg QE g-1 extract) was maximally reported in ethyl acetate. Highest antioxidant activities like total antioxidant (910mg AAE g-1), DPPH radical scavenging (IC50=57.26 μg ml-1), Superoxide radical scavenging (IC50=84.18μg ml-1), Hydrogen peroxide scavenging (IC50=74.34μg ml-1) and protein protective activity against free radical inducer AAPH were observed in S-3 methanolic root extract whereas FRAP (14.67 AAE g-1 extract) and Metal ion chelation (IC50=107.65μg ml-1) was best in S-3 acetone root extract. The highest number of compounds in S-3 root- (36) and leaf- (34) extracts were identified through GC-MS analyses with patchouli alcohol (22.92%) and α-Cadinol (14.17%) as predominant compounds. Highest content of Patchouli Alcohol (23.36 mg g-1 dry weight) was reported in S-3 root methanolic extract. Micronutrient analysis showed that Fe, Ca and Zn (19.95ppm, 10.30 ppm and 0.721 ppm) were highest in S-3 roots and Mn (4.27 ppm) in S-3 leaf was highest amongst the samples of different seasons. All the S-3 root extracts showed lowest MIC (15.62 & 31.25 μg) against gram positive L. monocytogenes and gram negative S. typhi, strains respectively. The maximum inhibition zone diameter (IZD) at par to ampicillin standard against 5 human pathogenic strains i.e., S. aureus (17.1 mm), B. cereus (16 mm), E. coli (8 mm), L. monocytogenes (14 mm) and S. typhi (10 mm) was recorded in S-3 ethyl acetate. The S-3 methanolic root extract showed maximum in vitro cytotoxic potential against three cancer cell lines viz. PK-15 (IC50=55.92μg ml-1), MDCK (IC50=64.55μg ml-1 ) and HEK (IC50=57.71μg ml-1). Results clearly revealed presence of significant amounts of phytochemicals and antioxidant activities due to variety of metabolites in leaves and roots and also in vitro cytotoxic potential against human pathogenic strains and cancer cell lines in roots. The multiutility of this medicinial herb against numerous pathological ailments was attributed to pharmacological activities.
  • ThesisItemOpen Access
    Seasonal variation in the nutritional and phytochemical characteristics of Grewia optiva leaves, flowers and fruits
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-02) Joshi, Prerna; Kumar, Vandana A.
    The multipurpose agroforestry tree species Grewia optiva, Bhimal (family Tiliaceae) distributed in mid-hills of north-western, western and central Himalayas (500-2500 m asl) is highly used for its green nutritious fodder during dry winter season. The three types of leaves L1 (soft, light green-coloured small-sized leaves), L2 (semi-hard, green-coloured medium-sized leaves) and L3 (hard,dark-green coloured medium-sized leaves) collected in six different growth stages (S1 through S6) varied from vegetative to reproductive phases. The S2L3 and S1L3 leaves showed the highest total soluble aqueous protein content (25.65±0.63 mg/g ) and total carbohydrate content (29.945 ± 6.12 mg/g ) respectively, whereas the total carbohydrate in flower (S3FL) and fruits (S4FR) were 4.45±0.14 mg/g and 16.99± 3.37 mg/g, respectively. The S4L3 had highest Mg content (69.1 mg/g), but Zn content (85.3μg/g) in it was lesser than that in S2L3 (89.4 μg/g). The Ca in S6L3 (39.22 mg/g), Cu in S5L3 (9.6μg/g) and Mn (222.2 μg/g) in S2L3 were the highest. The flower (S3FL) had better Mg (1.73 mg/g), Ca (14 mg/g), Cu (13.6 μg/g) and Mn (48.5 μg/g) than fruit (S4FR) which had higher Zn (17.1 μg/g) content than flower. Leaves of S6 showed highest TPC (166.46±2.46 mg GAE/gE) and FRAP (83.33±2.73 mg AAE/gE) and hydroxyl radical scavenging activity (IC50=393.92±0.99μg) whereas S4L3 showed highest TFC (20.329±0.576 mgQE/gE) and TAA (474.21±8.35 mg AAE/gE) suggesting that the antioxidant activity increased with decrease in temperature. The flower (S3FL) possessed greatest TPC (880.08±5.97 mg GAE/gE), TFC (137.45±2.37mg QE/gE), TAA (807.54±5.76 mg AAE/gE), FRAP (299.16±10.5mg AAE/gE) and hydroxyl radical scavenging activites (IC50= 309.022±1.12μg) than those of fruits and leaves. The TAA, FRAP and hydroxyl radical scavenging activity of flower was >2 times, > 4 times and >1.4 higher than average value of those of L3 leaves in 6 growth stages. The highest antioxidant enzyme activity of 0.105±0.004 U mg protein -1, 0.441 ± 0.005 U mg protein-1 and 6.507±0.05 U mg protein-1 were reported for CAT, SOD and POD in S6L3 indicating that the tree developed low temperature stress tolerance. Based on the nutritional and antioxidant qualities, G. optiva leaves, flowers and fruits were proposed to be used in late winter season as fodder when other sources of green fodder were unavailable which would benefit livestock.
  • ThesisItemOpen Access
    Comparative analysis of the biochemical parameters of wheat and buckwheat
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-02) Arya, Kiran; Agrawal, Sanjeev
    Cereal and cereal based food products constitute a large part of human diet. As per review, buckwheat flour may enrich the functionality of cereal based products. To derive the implications for possible improvement, 19 buckwheat genotypes and 5 wheat genotypes were studied for biochemical parameters. The average protein content of buckwheat genotypes (10.77%) was found to be higher than wheat (10.2%). The average phenol content in buckwheat (4.9 mg/g) was 4 and 7 times higher in coloured wheat and normal wheat, respectively. The average flavonoid content (3.12 mg/g) in buckwheat was 3 and 4 times higher in coloured wheat and normal wheat, respectively. The average IC50 of buckwheatgenotypes (56.9 μg) was 3.2 times and 5.3 times less as compare to coloured wheat and normal wheat. Low IC50 value indicates better antioxidant potential. The antioxidant activity is directly correlated with phenol and flavonoid content. The average mineral content i.e. magnesium, iron, zinc, copper and calcium in buckwheat were found to be less than wheat. It may fulfill the partial requirement of minerals in place of wheat in coeliacpatients. Our finding suggest that addition of buckwheat flour in wheat based product may improve the protein content and quality as well as antioxidant potential which would be beneficial for health point of view.