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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Plasma nanoparticles treated seed induced changes in molecular and biochemical profiling of wheat grown under heat and water stress condition
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-08) Gandhi, Vikas; Pandey, Priyanka
    Wheat is one of the main staple foods in India as well as in the world. The yield and grain quality of the crop are severely affected by stress conditions like heat and water stress. In the present study experiments were conducted to delineate the effect of plasma and microbial consortia under heat stress condition and effect of nano ZnO, nano chitosan and microbial consortia under water stress condition. Field experiments were done at Norman E. Borlaug Crop Research Centre of G. B. Pant University of Agriculture and Technology, Pantnagar in 2018-19, using Randomised Block Design. The “HD 2967” variety of wheat was used for seed treatment, sampling for molecular and biochemical profiling was done at three different growth stages i. e., booting, heading and grain filling stage. In both heat and water stress conditions, the contents of photosynthetic pigment, relative water content, protein, proline, sugar, and antioxidants were found to be highest in the combination plasma + MC I and nano ZnO + chitosan NPs + MC I treatment respectively, which helped wheat combat water stress condition. The grain protein, quality of grain and the yield were also best in this treatment. Maximum increase in Zn and Fe concentration was also recorded using this treatment. In case of heat stress condition, similar results were found for all the above parameters in case of plasma + MC 1 treatment. As the immunity of plant decreases during abiotic stress condition they become more susceptible to attack by biotic factors reducing plant growth, development and yield. So antimicrobial property of nano ZnO and chitosan nanoparticle was assessed in antifungal and antibacterial experiments. Both nano ZnO and nano chitosan were found to have inhibitory effect, but the combination of these two nanoparticles was observed to have maximum antimicrobial properties both in case of bacteria and fungi. Our research finding reveals that nano ZnO, nano chitosan and combination of both nanoparticles can be used to reduce the losses caused by abiotic stress in wheat.
  • ThesisItemOpen Access
    Evaluation of anti-fungal, growth promoting anti-oxidative and defense related activity of copper nanoparticles in Brassica juncea against Alternaria blight disease
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-07) Mann, Avni; Pandey, Dinesh
    Alternaria blight is one of the most catastrophic fungal diseases which hamper the productivity of Brassica oilseeds in India. Alternaria brassicae is the main pathogen of the disease which produces chlorotic, necrotic toxins and phyto hormones to cause the disease. At present the disease is controlled mainly by using toxic chemicals such as fungicides which have hazardous effect on environment and human health. So, nano technological interventions are necessary to find out some novel kind of substances that can act as anti –microbials because nanoparticles have high surface area with respect to volume. The surface area of nanoparticles is directly proportional to their interaction with the microbes to carry out antimicrobial activities. Copper based compounds (Bordeaux mixture) have been used as fungicides since ancient times. Therefore, copper nanoparticles (CuNPs) can act as effective antimicrobial agents. In present study it was observed that disease index during Alternaria blight disease was reduced to 22% on application of 75 ppm as compared to 86% control untreated pants. CuNPs induces the expression of COI1, receptor of Jasmonic acid but does not modulate signal transduction pathway because of absence of JA signal so Defensin gene expression is not triggered. Under effect of CuNPs Cu/Zn SOD, anti-oxidant enzyme gene was significantly over expressed. Along with anti – oxidative roles, Copper nanoparticles have anti-fungal properties too. It was observed that 75 ppm CuNPs cause a reduction of 23% during Alternaria growth and 71% inhibition during spore germination due to higher penetration power than salts, CuNPs shows high inhibitory effect on growth and development of Alternaria by damaging their protein structure and cell membrane 75ppm of CuNPs showed substantial growth promotory effect on seed germination, plant height and fresh weight by increasing anti-oxidant enzyme activities. The reason behind decrease in disease deverity could be anti-oxidantive activity of CuNPs. ROS produces by hypersensitive reactions on infection leads to oxidative stress in plants. The anti – oxidative enzymes scavenges these ROS. Hence CuNPs help in faster scavenging of ROS by activating anti – oxidant genes and enzymes inside the cell. CuNPs shows growth promoting activities also and thus help plants to escape the pathogen infecting stage at higher rate. CuNPs have significant effect on reducing disease severity of Alternaria blight in Brassica by trigerring anti-oxidative machinery of plant cell. Therefore CuNPs based formulations can effectively be utilized to counter Alternaria blight of Brassica. This would help in increasing the yield of oilseeds by reducing the losses due to disease.
  • ThesisItemOpen Access
    Studies on cytopathogenicity of egg drop syndrome-76 virus
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2005-08) Srivastava, Gaurangi; Rao, V.D.P.
    In the present investigation, an attempt was made to study the cytopathogenicity of Egg drop Syndrome-76 (EDS-76) virus. In this study, VN1 isolate of EDS-76 virus was cultivated in 9-10 days old duck embryo. When infected allantoic fluid was given to laying birds as an antigen, affected birds appeared dull and depressed with diarrhea. The important characteristic was drop in egg production coinciding with production of soft and thin shelled eggs. The virus was isolated from infected allantoic fluid in primary chicken embryo liver (CEL) cell culture. The cytopathic effects characterized by rounding and degeneration of cells were evident from the first passage itself. May Grunwald and Giemsa stained preparations of infected CEL cells culture revealed eosinophilic intranuclear inclusion bodies with distorted nuclei. AGPT performed using infected duck allantoic fluid and standard and experimentally raised hyperimmune sera against EDS-76 virus, revealed precipitin lines. But CIE was unable to detect the presence of virus in infected duck allantoic fluid and infected cell culture supernatants. FAT performed to detect the presence of EDS-76 virus in infected CEL cell culture exhibit intense intranuclear yellow fluorescence. The haemagglutination property of the virus was studied in infected duck allantoic fluid and infected CEL cell culture. The HI test was performed using hyperimmune serum to confirm the specificity of HA test and a 100% correlation was obtained.
  • ThesisItemOpen Access
    Molecular Cloning, Sequencing and In-silico Analysis of N, P, C domains and full length Calreticulin-3 gene of Brassica juncea for understanding its role in downstream regulation
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-02) Meenu; Pandey, Dinesh
    Rapeseed and mustard (Brassica juncea) holds immense agricultural and economic importance. The productivity of rapeseed and mustard is severely affected by Alternaria blight disease which is caused by the fungal pathogen, Alternaria brassicae. So far there is no source of resistance against this disease. However, biotechnological approaches could be used to develop disease resistance provided that the key candidate genes/proteins involved in imparting defence towards A. Brassicae pathogen are identified. In the recent past, the role of calreticulin has been demonstrated in during defence responses of plants towards various pathogens. Therfore it could prove an attractive candidate for improving defense response of Brassica plants towards Alternaria blight disease. Hence, the present studies were conducted to understand the similar role of Brassica juncea calreticulin-3 with the objective of isolating and cloning of N, P and C domains and full length Calreticulin-3 gene of Brassica juncea. Total RNA was isolated from 30-day old leaves of B. juncea var. PAB9511 followed by synthesis of cDNA and its confirmation by amplification of internal control Actin. The primers were designed from the mRNA sequence of Calreticulin-3 of Brassica rapa using Primer3 and BLAST tools. The PCR conditions were optimised for obtaining 1300 bp, 645 bp, 288 bp and 339 bp amplicons of full length calreticulin-3, N, P and C domains respectively. The N, P, C domains and full length calreticulin-3 gene were cloned into TA cloning vector, pGEMT-Easy vector and confirmed by colony PCR and restriction digestion using enzymes Xba I and EcoR I . The full length calreticulin 3 gene along with N, P and C domains were sequenced. The restricted fragments of each insert were then successfully cloned into mammalian expression vector, pCDNA3.1 and again confirmed by colony PCR and restriction digestion. The BLASTn results revealed that the full length calreticulin-3 of Brassica juncea showed 98% sequence identity with the mRNA sequence of calreticulin-3 of Brassica rapa subspecies pekinensis. Also, the phylogenetic tree revealed more than 90% sequence similarity between calreticulin-3 of Brassica juncea to the calreticulin-3 of Brassica rapa, Brassica napus and Brassica oleracea. The SmartBLAST analysis revealed 86% homology between calreticulin-3 protein of Brassica juncea and Arabidopsis thaliana. The domain analysis assured the presence of calreticulin domain in this sequence and motif analysis revealed the presence of many motifs having significant involvement in responding to various biotic and abiotic stresses. The in-silico prediction of phosphorylation sites revealed the presence of 17 serine residues, 5 tyrosine residues and 9 threonine residues that could be involved in phosphorylation/ dephosphorylation indicating their involvement in signaling transduction.The prediction of interacting partners of calreticulin-3 of Brassica rapa showed that it could interact with 70 KDa heat shock protein, Brassinosteroid insensitive-1 and dolichyl-diphospho-oligosaccharide-protein glycotransferase. The role of these proteins in plant response to various stresses has been demonstrated by recent studies. These could also be hypothesized to be the interacting partners for calreticulin-3 of Brassica juncea. The experimental studies of effect of over expression of calreticulin-3 in Brassica plants could be tested for its role in conferring resistance against Alternaria blight and other different pathogenic infections or environmental stresses.
  • ThesisItemOpen Access
    Comparative expression analysis of defense-related genes in both wild and transgenic Brassica juncea (var.) Varuna harboring mapk3 gene in response to infection by Albugo candida
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-05) Modak, Annayasa; Gohar Taj
    Mustard and Rapeseed are the third leading source of vegetable oil in the world after soybean and oil palm. According to Department of Agriculture & Farmers welfare 2017-2018 annual production of Rapeseed and mustard is 75.40 Lakh tones. Production in India is still not enough to meet the domestic requirement. The potential yield of Brassica is very high but there is a wide gap between the potential and realized yield of Brassica and it is largely because of biotic & abiotic stresses. White rust is one of the most important disease of Brassica and it is caused by pathogen Albugo candida.Even 60% reduction in yield of Brassica juncea is due to its high susceptibility to white rust. Plants defend themselves against most potent pathogens through various defense mechanisms. Activation of MAPK signalling cascade is one of major defense pathways by which extracellular stimuli are transduced into intracellular responses in all the eukaryotes. MAPK cascade also play key role in regulation of various defense related genes in response to pathogens. Therefore our investigation was to analyse expression level of potential MAPK cascade and defense related genes in wild and transgenic Brassica juncea (var.) Varuna harboring overexpressed MAPK3 gene to access tolerance against Albugo candida. Lower disease index in transgenic Brassica suggests its tolerance to Albugo candida. Higher expression level of MAPK6 than MAPK3 in the transgenic Brassica suggests that MAPK6 mimics a portion of MAPK3 signalling pathway and work in a direct cascade for production of defense related proteins. Overexpression of MAPK3 gene was observed in downregulating the expression of MAPK4 in transgenic Brassica juncea . The expression of WRKY33 and WRKY29 was also analysed in the present study. Expression levels of both WRKY33 and WRKY29 were found to be higher in transgenic plant overexpressing MAPK3. Expression of 3 Defense related genes (OASTL-B, ACD2, and CSD2) were found higher in transgenic plants horboring overexpressed MAPK3 gene & expression level of CYP20-3 was found higher in wild Brassica plant. The defense related genes (OASTL-B, ACD2, CSD2, and CYP20-3) were also subjected to In-silico study. Insilico study showed the Secondary structure, tertiary structure and putative phosphorylation sites of these 4 defense related genes & also derived protein-protein interaction of potential MAPKs cascade and WRKY (29, 33) with OASTL-B, ACD2, CSD2 & CYP20-3.
  • ThesisItemOpen Access
    Molecular analysis of Osdof 1 transcription factor in response to nitrogen and abiotic stress treatments in rice (Oryza sativa L.)
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Khushboo, Manjari Mala; Singh, B.R.
    Abiotic stress such as draught (water deficeiency), excessive watering , extreme temperature (such as cold ,frost, and heat ), salinity , and poor nitrogen use efficiency are major limiting factor which give negative impact to growth , yield and seed quality. Rice become more sensitive toward draught during- booting( most critical stage), tillering and primordial stage of its growth , inspite of its sensitivity the poor nitrogen uptake by the rice i.e only 30-40% of the applied nitrogen fertilizer which drastically effect the yield of the crop. Due to this reason , in last 40 year , the amount of synthetic nitrogen (N) applied to crop has risen dramatically, from 12T g/year to 104T g/year resulting in increase in yield but with considerable impacts on global environment because plants consume much less than half of the applied , means more then half of the fertilizer are leach into surrounding lake , ponds , river and cause environmental problem .Taking consideration of these problem,the aim of the study was determination of change in physiological parameter viz. plant height , leaf area and root length , biochemical parameter viz. total chlorophyll content, proline content and total soluble sugar ,transcription factor viz. dof1 , and lastly transporter AMT1;1 was studied. Change in this parameter directly effect the yield of the crop. When plant suffer to any abiotic stress the injury which observe firstly via naked is on leave. Stress due to salt greatly influence the leaf area while stress due to nitrogen deficiency mainly effect on chlorophyll. After a period of stress duration root physiology also get affected ,upto certain concentration of nitrogen deficiency ,elongation in root length was observe but in case of salinity stress shrinkage in root length was observe .Increase in proline (as compare to other amino acid) and sugar concentration can be taken as biochemical parameter for plant under stress . By accumulation of these things ossmoticum of cell get influence, which help the plant to overcome during stress condition. However increase in proline is more influence during stress as compare to free sugar. After this different morphological and biochemical parameter, dof1 expression was analysed to know its relation with nitrogen .The expression fold of Dof1 decreases with decrease in concentration of nitrogen.
  • ThesisItemOpen Access
    Study on synthesis, characterization and effect of silica nanoparticles on drought tolerance in Eleusine coracana
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-06) Snehal, Sikha; Lohani, Pushpa
    Drought is a major abiotic stress factor limiting crop productivityworldwide. Finger millet (Eleusine coracana) is a hardy crop that can withstand variant degree of drought stress. The physiological and biochemical responses of finger millets to the different concentrations of silica nanoparticles (0, 25, and 50 mg/L) and two soil moisture treatments (without stress and severe stress) were investigated. Application of the SNPs was done for 45 days before exposing them to5 days of drought stress. Morphohysiological parameters and biochemical parameters such as malondialdehyde (MDA), relative water content (RWC), chlorophyll, carotenoid, and proline content were determined. Under drought conditions, the effect of silica nanoparticles on the morphophysiological and other biochemical was evident. A decrease in ROS production and an enhancement in the antioxidant system for Silica nanoparticles applied plants may account for their increased tolerance to abiotic stresses. Application of silica nanoparticles decreased O2¯, H2O2, and MDA concentrations in finger millets leaves by increasing the activities of ascorbate peroxidase (APX) dehydroascorbatereductase (DHAR), or glutathione (GSH) reductase (GR) under water stress. A significant increase in the relative water content (RWC), chlorophyll, carotenoid, and proline content was observed in the PES-400 genotype in which silica nanoparticle was applied. Hence, it was concluded from this study that application of silica nanoparticles confers drought tolerance property even in the drought sensitive variety of finger millet PES-400.
  • ThesisItemOpen Access
    Characterization of EMS induced mutants of hexaploid wheat (Triticum aestivum L.) using morphological and physiological parameters
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-07) Ghosh, Saipayan; Sundip Kumar
    Wheat (Triticum aestivumL.) is one of the most important cereal crops cultivated all over the world. It is the staple food for 1.2 billion people of the world.With the increasing population, improvement of both production and quality of crop is very important. Hence,during the present investigation, previously developed mutant population was screened for lodging tolerance with high yielding property including a previously selected wheat mutant (M1) which was characterized by growing under different plant density, spacing and nitrogen gradients in comparison to control plants i.e. DPW 621-50 and HD-2967. The mutant line (M-1) was found to have superior performance in terms of lodging tolerance and high tillering capacity without compromising yield in comparison to both the controls (DPW 621-50 and HD 2967) in all the experimental conditions used in the present investigation. Therefore, this mutant may directly be utilized as a cultivar followed by its evaluation under AICRP/State Varietal Trials or may also be utilized as a donor parent for the development of improved lodging tolerant and high tillering genotype. In addition to this the morphological and lodging tolerant characteristics of 140 mutant lines were studied and 13wereselected as putative lodging tolerant mutantsfor subsequent utilization in the development of lodging tolerant wheat. In order to improve the mineral nutritional quality of wheat grains, the newly developed 64 mutants lines were also screened for grain Ca, Zn, Cu and Fe contents. To overcome the concentration effect, normalization of the mutants was done by selecting the mutants having 1000 grain weight equivalent to control or higher. The mutant line M-470 was observed to have high contents of Fe (66.70 ppm), Zn (47.25 ppm) and Cu (8.80 ppm). Therefore the mutant line M-470 can be utilized as a donor parent in wheat biofortification programme for the development of wheat genotypes with high Fe, Zn and Cu and M-471 with Ca content of 338.817can be utilized as a donor parent for the development of Ca biofortified wheat.
  • ThesisItemOpen Access
    Evaluation of antioxidative potential and cytokines mediated immunopotentiating activity of Eclipta alba (L.) Hassk. in chicken lymphocytes culture system
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Sharma, Raunak; Ambwani, Sonu
    Medicinal plants have gained popularity in recent era and there has been a rise in the researches related to them. They possess numerous antibacterial, antioxidants and anticancer agents which provide an alternative mode of therapy to several diseases and infections caused by drug resistant pathogenic strains, oxidative stress, etc. Eclipta alba (L.) Hassk., also known as ‘false daisy’ or ‘bhringraj’ belongs to family Aesteraceae. Since ancient times, this plant has been used in Ayurveda. The fact that human body requires additional nutrients, has given rise to consumption of eggs and meat in the country. Thus, health of poultry is important aspect as consumption of healthy poultry products will enhance the health of human population as well. On the above mentioned facts, present study was planned to explore immunomodulatory and antioxidative potential of 50% hydromethanolic extract of aerial part of Eclipta alba (EAE). Extract of the plant was prepared and evaluated for the presence of various phytoconstituents through qualitative and quantitative phytochemical analyses. DPPH and NO scavenging assays were carried out to assess the antioxidative potential of EAE. The immunomodulatory potential of EAE was evaluated through lymphocyte proliferation assay (LPA) followed by biochemical analyses (LPO, GSH, SOD, catalase and NO) in EAE treated chicken lymphocytes. Expression analysis of IL-6, IL-10 and iNOS was carried out through quantitative real time PCR in chicken lymphocytes to assess role of EAE exposure in modulating their expression. The extraction yield of EAE was found to be 8.51%. Biochemical analyses revealed the presence of various phytoconstituents. The total phenolics content and total flavonoid content in EAE was estimated to be 871.11 mg/g and 376 mg/g, respectively. Maximum non-cytotoxic doses of both, EAE and dexamethasone were determined in the lymphocytes culture system which was found to be 75μg/ml for EAE and 30ng/ml for dexamethasone. EAE displayed significant increase of 14.31% and 33.67% in T cell proliferation in case of PHA and Con A stimulated cells, respectively. There was marked increase of 30.64% in B cell proliferation in LPS stimulated cells as compared to control. The antioxidant property of the extract was revealed by DPPH assay and NO scavenging assay. The IC50 value for EAE was found to be 175.16 μg/ml in DPPH assay and 70.39 μg/ml in NO scavenging assay. There was significant decrease in the percent Lipid peroxidation and NO content after the treatment with the EAE in chicken lymphocytes. However, GSH, SOD and Catalase levels were significantly increased in the cells treated with EAE in comparison to control cells. The expression of IL-6 was found to be increased in Con A stimulated EAE treated cells, but the same was decreased in LPS stimulated EAE treated cells. iNOS expression was found to be reduced significantly in macrophages rich cells as well as in Con A and LPS stimulated EAE treated cells. However, expression of IL-10 was elevated significantly in Con A stimulated EAE treated lymphocytes but was reduced in LPS stimulated EAE treated lymphocytes. The findings of this study indicated that the hydromethanolic extract of aerial parts of Eclipta alba are rich in phytochemicals (flavonoids and phenolics). Both the extract and dexamethasone exhibited presence of antioxidantive potential and displayed immunomodulatory effects in chicken lymphocytes culture system. EAE exposure modulated the expression of IL-6, IL-10 and iNOS in chicken splenocytes. Due to the significant immunopotentiating and antioxidative potential of EAE, it could be used for development of immunoenhancing herbal preparations for poultry. However, exhaustive research is needed to characterize phytoconstituents and the findings need to be validated at protein level by employing modern biochemical and molecular biology techniques. Also the effects of Eclipta alba need to be determined on suitable in vitro and in vivo systems.