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National Dairy Research Institute, Karnal

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2018 - 20199
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Subject: Veterinary Gynaecology and Obstetrics × End date: 2019 × Start date: 2018 × Type: Thesis ×
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Now showing 1 - 9 of 9
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    ThesisItemOpen Access
    IDENTIFICATION OF NOVEL TRANSCRIPTS AND METABOLITES IN SPERMATOZOA OF MALNAD GIDDA
    (ICAR-SRS-NDRI, KARNAL, 2019) GUPTA, MOHUA DAS; KUMARESAN, A.
    Breed variations in fertility have been reported in literature with comparatively lesser infertility in zebu breeds as compared to other breeds. While the problem of infertility is comparatively higher in crossbred males as compared to pure bred males, the reason for the same has not been elucidated yet. We hypothesized that comparative analysis of molecular profile of spermatozoa from dwarf zebu and crossbred bull might help in identification of possible alterations in spermatozoa of crossbred bull. On the other hand, the possible molecular pathways associated with high fertility in dwarf could also be identified. With this back drop, global transcriptomic and metabolomic profile of a dwarf zebu (Malnad Gidda) and crossbred (HF crossbred) bull spermatozoa was carried out. A total of 15814 and 17324 transcripts were identified in dwarf zebu bull spermatozoa and crossbred bull spermatozoa respectively, of which 521 transcripts were differentially expressed between dwarf zebu bull and crossbred bull spermatozoa. Among the differentially expressed transcripts 156 were upregulated while 365 were down regulated in dwarf zebu bull spermatozoa as compared to crossbred bull spermatozoa. Olfactory transduction pathway was upregulated whereas Ribosomal pathways, Oxidative phosphorylation and metabolic pathways were downregulated in dwarf zebu bull spermatozoa. Metabolomics analysis of sperm and seminal plasma revealed 1372 and 1240 metabolites in dwarf zebu bull and crossbred bull spermatozoa, respectively of which 71 metabolites were differentially expressed between dwarf zebu bull and crossbred bull spermatozoa respectively. A total of 1002 and 990 metabolites were identified in dwarf zebu bull and crossbred bull seminal plasma, respectively; of which 6 metabolites were downregulated in dwarf zebu bull spermatozoa as compared to crossbred bull spermatozoa. A metabolite Nitroprusside was 2.6 fold higher whereas LCysteine and Acetyl-CoA were 2.4 fold lower in dwarf zebu bull as compared to crossbred bull spermatozoa whereas, Tetradecanoyl-CoA and Phosphotidylethanolamine were 3.5 and 3 fold lower in dwarf zebu bull seminal plasma as compared to crossbred bull seminal plasma. It is concluded that transcriptional abundance of genes associated with Olfactory transduction were significantly higher in dwarf zebu bull spermatozoa as compared to crossbred bull spermatozoa. Expression of transcripts involved in ribosome pathway and oxidative phosphorylation were significantly upregulated in crossbred bull spermatozoa as compared to dwarf zebu bull spermatozoa. A total of 2 novel transcripts and 3 noncoding RNAs were identified in dwarf zebu bull spermatozoa. Taurine and hypotaurine metabolism pathway were highly enriched and concentration of nitroprusside was significantly higher in dwarf zebu bull spermatozoa as compared to crossbred bull spermatozoa. These findings might help in understanding the reasons for high incidence of infertility/ subfertility in crossbred bulls and to improve the quality of cryopreserved spermatozoa.
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    ThesisItemOpen Access
    Studies on cryosurvivability of low sperm doses and in vitro sperm function tests in Sahiwal bulls.
    (ICAR-NDRI, Karnal, 2018) Lone, Shabir Ahmad; Mohanty, Tushar Kumar
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    Seasonal influence on expression of fertility associated genes and sperm-oviduct binding in buffaloes.
    (ICAR-NDRI, Karnal, 2018) Rather, Haneef Ahmad; Kumaresan, A
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    ThesisItemOpen Access
    TESTICULAR TRANSCRIPTOMIC ANALYSIS OF CROSSBRED AND INDIGENOUS CATTLE
    (ICAR-NATIONAL DAIRY RESEARCH INSTITUTE, BENGALURU, 2019) K, ELANGO.; KUMARESAN, A.
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    ThesisItemOpen Access
    EFFECT OF SEMEN FILTRATION ON LOW DOSE SPERM CRYO-SURVIVABILITY IN FMD VACCINATED CROSSBRED BULLS
    (ICAR-NATIONAL DAIRY RESEARCH INSTITUTE, KARNAL, 2019) BHANUBHAI, RAVAL KATHAN; Mohanty, T.K.
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    ThesisItemOpen Access
    EFFECT OF KISSPEPTIN ON ONSET OF PUBERTY IN MURRAH BUFFALO HEIFERS
    (ICAR-NATIONAL DAIRY RESEARCH INSTITUTE, KARNAL, 2019) PANDEY, HIMANSHU; Kumar, NISHANT
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    ThesisItemOpen Access
    ANTISPERM ANTIBODIES IN BOVINE: PREVALENCE AND RELATIONSHIP WITH FIELD FERTILITY
    (ICAR-NATIONAL DAIRY RESEARCH INSTITUTE, BENGALURU, 2019) KUNTAREDDI, CHANNAREDDI; KUMARESAN, A.
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    ThesisItemOpen Access
    Spermatogonial stem cells transplantation in crossbred bovine and swine males.
    (ICAR-NDRI, Karnal, 2018) Kumar, Sunil; Kumaresan, A.