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Kerala Veterinary and Animal Sciences University, Wayanad

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20182
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Subject: Veterinary Microbiology × Author: P. S. RESHMA × End date: 2019 × Start date: 2010 ×
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    ThesisItemOpen Access
    A COMPREHENSIVE APPROACH FOR DIAGNOSIS OF LEPTOSPIROSIS IN DOMESTIC PIGS
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2018-09-30) P. S. RESHMA; M. Mini
    Leptospirosis is a worldwide zoonotic disease that in pigs primarily causes reproductive disturbances. Samples collected from Centre for Pig Production and Research (CPPR), Mannuthy and selected private farms in Thrissur were used for the present study. Serum samples (n=103) were tested using microscopic agglutination test (MAT) and an overall seropositivity of 35.92 per cent could be detected. The serogroup Pomona (45.95 per cent) was the most prevalent among the 37 positive samples followed by serogroups Grippotyphosa (24.32 per cent), Canicola (13.51 per cent), Icterohaemorrhagiae (10.81 per cent) and Tarassovi (5.41 per cent). Samples of serum (n=56), whole blood (n=52) and aborted foetus (n=7) when tested using PCR for the presence of lipl32 gene of Leptospira, DNA of three whole blood samples and one aborted foetus amplified the gene producing an expected 767 bp amplicon. Latex agglutination test (LAT) and indirect fluorescent antibody test (FAT) was standardised using seven-day-old reference cultures of Leptospira interrogans serovar Pomona as positive controls at 10-fold dilutions. However, none of the abortion samples tested using LAT and FAT were positive. Attempts for isolation from positive samples were also made, but Leptospira could not be isolated from any of the foetal membrane, liver, kidney samples from the PCR positive animals. Hence in the present study, a seroprevalence of 35.92 per cent for leptospirosis among pigs in Thrissur district was detected using MAT, and PCR was found to be the most sensitive method for directly detecting the presence of the organism in clinical samples, compared to LAT, FAT and culture.
  • Thumbnail Image
    ThesisItemOpen Access
    A COMPREHENSIVE APPROACH FOR DIAGNOSIS OF LEPTOSPIROSIS IN DOMESTIC PIGS
    (College of Veterinary and animal Science,Mannuthy, 2018) P. S. RESHMA; M. Mini
    Leptospirosis is a worldwide zoonotic disease that in pigs primarily causes reproductive disturbances. Samples collected from Centre for Pig Production and Research (CPPR), Mannuthy and selected private farms in Thrissur were used for the present study. Serum samples (n=103) were tested using microscopic agglutination test (MAT) and an overall seropositivity of 35.92 per cent could be detected. The serogroup Pomona (45.95 per cent) was the most prevalent among the 37 positive samples followed by serogroups Grippotyphosa (24.32 per cent), Canicola (13.51 per cent), Icterohaemorrhagiae (10.81 per cent) and Tarassovi (5.41 per cent). Samples of serum (n=56), whole blood (n=52) and aborted foetus (n=7) when tested using PCR for the presence of lipl32 gene of Leptospira, DNA of three whole blood samples and one aborted foetus amplified the gene producing an expected 767 bp amplicon. Latex agglutination test (LAT) and indirect fluorescent antibody test (FAT) was standardised using seven-day-old reference cultures of Leptospira interrogans serovar Pomona as positive controls at 10-fold dilutions. However, none of the abortion samples tested using LAT and FAT were positive. Attempts for isolation from positive samples were also made, but Leptospira could not be isolated from any of the foetal membrane, liver, kidney samples from the PCR positive animals. Hence in the present study, a seroprevalence of 35.92 per cent for leptospirosis among pigs in Thrissur district was detected using MAT, and PCR was found to be the most sensitive method for directly detecting the presence of the organism in clinical samples, compared to LAT, FAT and culture.