Thumbnail Image

Kerala Veterinary and Animal Sciences University, Wayanad

Browse

20211
Reset filters
Subject: Veterinary Gynaecology and Obstetrics × Author: AMBILY K G × End date: 2021 × Start date: 2020 × Thesis Type: M.V.Sc. ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    FREEZABILITY OF SPECIFIC FRACTIONS OF LARGE WHITE YORKSHIRE BOAR SEMEN SUPPLEMENTED WITH SODIUM DODECYL SULPHATE
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2021-12-30) AMBILY K G; Hiron M. Harshan
    The present research was conducted to evaluate the effect of boar semen fraction as well as supplementation of sodium dodecyl sulphate (SDS) on its freezability. Adult healthy Large White Yorkshire boars aged between 18 to 24 months, maintained at the Centre for Pig Production and Research, Mannuthy were selected for the study. The study was conducted in two phases, phase I for evaluating quality of semen fractions and phase II to evaluate effect of fractions and SDS on cryopreservability. The first 10 mL of sperm rich fraction (SRF) was designated as F1 and the rest of the SRF as F2. A total of 39 ejaculates were collected for the present study (phase I- 27, phase II- 12). In phase I the F1 fraction had significantly lower (p<0.01) pH than F2 and higher concentration (p<0.01) than F2, but were similar in protein concentration and protein bands on SDS PAGE analysis. In phase II the fractions after collection were immediately divided into three parts, namely, A (half of F1), B (half of F2) and C (remainder of F1 and F2 mixed). These were further processed and divided into six groups based on the fraction used and the presence or absence of SDS in the extender. The extender used for cryopreservation was Lactose egg yolk extender (LEY) with or without SDS. The extended semen was subjected to manual cryopreservation at a concentration of 1000 × 106 in 0.5 mL French Cassou straws. The protocol involved a holding time of one hour at 18C and an equilibration period of 90 min at 5C. Fresh semen characteristics such as sperm progressive motility, viability, abnormality, acrosome integrity, plasma membrane integrity did not differ among fractions, whereas, concentration (p<0.05), functional membrane integrity (p<0.05) and sperm membrane cholesterol content (p<0.01) varied significantly between fractions. Post-thaw evaluation revealed that sperm from F1 had better post-thaw characteristics than F2. Considering all the parameters, sperm from F1 had better post-thaw progressive motility than the F1 and F2 mixed group (which represented SRF). On evaluation of supplementation of SDS, it was found that SDS supplemented F1 was better in all parameters and had significantly higher HOS response than F1 extended without SDS. The cholesterol content of sperm in F1 was significantly higher than F2 during all stages of preservation (p<0.01) Thus it could be concluded that fraction F1 (first 10 mL of SRF, had better cryopreservability than F2 or whole of SRF) and incorporation of SDS was found to yield better post-thaw HOS response. Hence use of F1 fraction for cryopreservation of boar semen, with supplementation of SDS can yield better cryopreservability of LWY boar semen.