FREEZABILITY OF SPECIFIC FRACTIONS OF LARGE WHITE YORKSHIRE BOAR SEMEN SUPPLEMENTED WITH SODIUM DODECYL SULPHATE
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Date
2021-12-30
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR
Abstract
The present research was conducted to evaluate the effect of boar semen fraction
as well as supplementation of sodium dodecyl sulphate (SDS) on its freezability. Adult
healthy Large White Yorkshire boars aged between 18 to 24 months, maintained at the
Centre for Pig Production and Research, Mannuthy were selected for the study. The
study was conducted in two phases, phase I for evaluating quality of semen fractions and
phase II to evaluate effect of fractions and SDS on cryopreservability. The first 10 mL of
sperm rich fraction (SRF) was designated as F1 and the rest of the SRF as F2. A total of
39 ejaculates were collected for the present study (phase I- 27, phase II- 12). In phase I
the F1 fraction had significantly lower (p<0.01) pH than F2 and higher concentration
(p<0.01) than F2, but were similar in protein concentration and protein bands on SDS PAGE analysis. In phase II the fractions after collection were immediately divided into
three parts, namely, A (half of F1), B (half of F2) and C (remainder of F1 and F2 mixed).
These were further processed and divided into six groups based on the fraction used and
the presence or absence of SDS in the extender. The extender used for cryopreservation
was Lactose egg yolk extender (LEY) with or without SDS. The extended semen was
subjected to manual cryopreservation at a concentration of 1000 × 106 in 0.5 mL French
Cassou straws. The protocol involved a holding time of one hour at 18C and an
equilibration period of 90 min at 5C. Fresh semen characteristics such as sperm
progressive motility, viability, abnormality, acrosome integrity, plasma membrane
integrity did not differ among fractions, whereas, concentration (p<0.05), functional
membrane integrity (p<0.05) and sperm membrane cholesterol content (p<0.01) varied
significantly between fractions. Post-thaw evaluation revealed that sperm from F1 had
better post-thaw characteristics than F2. Considering all the parameters, sperm from F1
had better post-thaw progressive motility than the F1 and F2 mixed group (which
represented SRF). On evaluation of supplementation of SDS, it was found that SDS
supplemented F1 was better in all parameters and had significantly higher HOS response
than F1 extended without SDS. The cholesterol content of sperm in F1 was significantly
higher than F2 during all stages of preservation (p<0.01) Thus it could be concluded that
fraction F1 (first 10 mL of SRF, had better cryopreservability than F2 or whole of SRF)
and incorporation of SDS was found to yield better post-thaw HOS response. Hence use
of F1 fraction for cryopreservation of boar semen, with supplementation of SDS can
yield better cryopreservability of LWY boar semen.
Description
Submitted in partial fulfilment of the requirement for the degree of
MASTER OF VETERINARY SCIENCE in Animal Reproduction, Gynaecology and Obstetrics