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Kerala Veterinary and Animal Sciences University, Wayanad

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2015 - 20162
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Subject: Preventive Veterinary Medicine and Epidemiology × End date: 2019 × Start date: 2010 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    COMPARATIVE EVALUATION OF INTRADERMAL AND SUBCUTANEOUS PROPHYLACTIC RABIES VACCINATION IN DOGS
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR, 2015) SEEMANTHINI R; Vinodkumar K
    The effectiveness of pre exposure prophylaxis against rabies in dogs by using inactivated tissue culture rabies vaccine administered through subcutaneous and intradermal route was compared. Twenty healthy, unvaccinated pups of age group 10 to 12 weeks brought to Teaching Veterinary Complex, Mannuthy and University Veterinary Hospital, Kokkalai were selected for the study under informed consent from the owners. Health status of pups was ascertained by clinical and haematological evaluation. Owners were advised to provide uniform environment and practice identical feeding practices as far as possible. On day 0, Group I were given one dose of inactivated tissue culture rabies vaccine through subcutaneous route by hypodermic needle and group II were given total of one-tenth dose of inactivated tissue culture rabies vaccine on medial aspect of each ear pinnae through intradermal route by using an instrument called DermojetR. Booster dose was administered for both the groups on day 30. Serum samples were collected on days 0, 15, 30, 45 and 180 post vaccination. The serum neutralizing antibody titres were assessed by Rapid fluorescent antibody test (RFFIT). All animals were having insignificant antibody titre by day 0. There was no significant difference in production of neutralizing antibodies between subcutaneous and intradermal groups after both primary and booster vaccinations. Animals in both the groups developed protective titre as early as day 15. Even though antibody peaked at 45th day, there was no statistically significant difference between 30th and 45th day of both the groups. Decline in antibody titre was evident after 45 days in both groups, but protective antibody titre was maintained all through the study period of 180 days in both groups. It is concluded that intradermal route of vaccination offered better economic prospects since similar antibody levels were produced and maintained with one-fifth dose of vaccine.
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    ThesisItemOpen Access
    FOOT AND MOUTH DISEASE VACCINATION – POST VACCINAL SEROCONVERSION IN CAPTIVE ASIAN ELEPHANTS (ELEPHAS MAXIMUS INDICUS)
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR, 2016) MARY VIJAYA; Vinodkumar K
    The present study was undertaken to determine the dosage of oil-adjuvant trivalent Foot and Mouth Disease (FMD) vaccine sufficient to develop protective antibody titre in adult healthy elephants, persistence of post-vaccinal antibody titre and to recommend a vaccination protocol against FMD in elephants. A total of 18 captive elephants under the custodianship of Department of Forests and Wildlife, Temples and private individuals from Thrissur, Ernakulam, Kollam and Trivandrum districts formed the materials for study. The owners were advised to provide uniform management to the animals as far as possible. Health status of the elephants was ensured prior to vaccinations by clinical examination and assessment of haemato-biochemical parameters. Presence of haemoparasites and gastro-intestinal parasites were ruled out by microscopic examination of blood smears and fecal samples. The animals were divided into three groups of six each and were subjected to prophylactic vaccination with three different doses i.e., six mL, eight mL and 10 mL of oil-adjuvant trivalent FMD vaccine (Raksha O vac Trivalent). Sera samples from the day of vaccination and 15th, 30th and 180th day post vaccination (dpv) were collected and subjected to Liquid Phase Blocking (LPB) ELISA to determine the development of antibody titre against FMD virus (FMDV) serotypes O, A and Asia-1. All the animals had non-protective titre against the three serotypes on day zero. Protective titres developed against all three serotypes on 30 dpv which declined gradually by 180 dpv in all elephants irrespective of the dose of the vaccine. None of the animals had protective titre by 180 dpv. It is concluded that six mL of vaccine is enough to produce protective titre in elephants against FMD. Further research is needed to study about the persistence of protective antibody titre following vaccination against FMD in Asian elephants.