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Kerala Veterinary and Animal Sciences University, Wayanad

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1994 - 19992
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Subject: Preventive Veterinary Medicine and Epidemiology × End date: 1999 × Start date: 1994 ×
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    ThesisItemOpen Access
    SEROPREVALENCE AND DIAGNOSIS OF CANINE PARVOVIRAL INFECTION
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCE,MANNUTHY, 1999) DEEPA. P. M.; Saseendranath, M R
    The prevalence of canine parvoviral infection in three hundred and forty nine dogs from all over Kerala including 57 clinically suspected dogs and 292 healthy and vaccinated dogs was studied. Among 57 clinically suspected dogs, 15.7 per cent and 19.3 per cent respectively, had CPV antigen in the faecal samples by AGID and CIEP. Canine parvovirus (CPV) antigen were detected in faecal samples of 61.4 per cent of 57 clinically suspected dogs by HA test. EM detected CPV in one (13 per cent) of eight clinically suspected dogs and 17 per cent of six clinically suspected dogs had CPV in the faecal samples by lEM. Agar gel immunodiffusion test detected canine parvoviral antibody in seven (12.3 per cent) of clinically suspected dogs, 14 (36.8 per cent) of 38 healthy vaccinated dogs and 17 (6.7 per cent) of 254 healthy non-vaccinated dogs. Counterimmunoelectrophoresis (CIE) test detected CPV antibody in 8 (14 per cent) out of 57 clinically suspected dogs 14 (36.8 per cent) of 38 healthy vaccinated dogs and 17 (6.7 per cent) of 254 healthy non-vaccinated dogs. Out of 349 dogs screened by HI test, 70.5 per cent dogs had the protective level of antibody against canine parvoviral infection. Haemagglutination inhibition test detected CPV antibodies in 36 (63.2 per cent) of 57 clinically suspected dogs, 37 (97.3 per cent) of 38 healthy vaccinated dogs and 173 (68.1 per cent) of 254 healthy non-vaccinated dogs. Dot-ELISA detected CPV antibodies in 38 (66.7 per cent) of 57 clinically suspected dogs, 37 (97.3 per cent) of 38 healthy vaccinated dogs and 174 (68.5 per cent) of 254 healthy non-vaccinated dogs. More seropositive dogs were recorded in dogs below six months of age. Prevalence rate observed was more in male dogs, eventhough no significant difference was observed between sexes. Among the breeds, German shepherd were more affected, followed by Doberman Pinscher and non-descript. Seasonal prevalence in the present study was higher during April, June and July. However, no significant difference was observed between different months.
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    ThesisItemOpen Access
    MODIFIED INTRA-DERMAL TEST FOR THE DIAGNOSIS OF PARATUBERCULOSIS IN GOATS
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES Mannuthy - Thrissur, 1994) VINOD KUMAR, K.; Saseedaranath, M.R.; VINOD KUMAR, K.
    Efficacy of single intra-dermal Johnin test was compared against two modified intra-dermal Johnin tests for diagnosis of paratuberculosis in goats. One hundred and fifty adult goats were divided at random into three groups and each group v^as subjected to one of the intra-dermal tests. Group I being the single Johnin intra-dermal (SID) Johnin test whereas in group II (modified intra-dermal test - MID-I) and group III (modified intra-dermal test - MID-II). The second injections were given after third and fifth days of first injection. Six animals giving positive results and two animals giving negative results were slaughtered from each group and confirmation of infection was ascertained by histopathology. Fecal samples and rectal pinch from all the animals were subjected to Zeihl-Neelsen's acid-fast staining for detection for M. paratuberculosis. Serum magnesium levels of all testpositive animals and an equal number of test-negative animals from each group were compared. After 24 h MID-I was found to be superior to both SID and MID-II tests. The MID-I test had higher specificity (66.6 per cent against 58.33 per cent in SID and 40 per cent in l^iiD-ii) and predictive value of positive results (83.3 against 55.5 in SID and 50 per cent in MID-II)• The MID-I and MID II tests had 100 per cent sensitivity and predictive value of negative results as against 50 per cent and 33.3 per cent of SID. Examination of fecal sample and rectal pinch was found to be of little value in the diagnosis of early paratuberculosis in goats. Serum magnesium levels between test positive and test negative animals was found to be not significantly different in all three groups. The results of the present study indicate that MID-I test could be used as an efficient diagnostic test for detection of paratuberculosis among goats.