MODIFIED INTRA-DERMAL TEST FOR THE DIAGNOSIS OF PARATUBERCULOSIS IN GOATS
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Date
1994
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES Mannuthy - Thrissur
Abstract
Efficacy of single intra-dermal Johnin test was
compared against two modified intra-dermal Johnin tests for
diagnosis of paratuberculosis in goats. One hundred and fifty
adult goats were divided at random into three groups and each
group v^as subjected to one of the intra-dermal tests. Group I
being the single Johnin intra-dermal (SID) Johnin test whereas
in group II (modified intra-dermal test - MID-I) and group III
(modified intra-dermal test - MID-II). The second injections
were given after third and fifth days of first injection. Six
animals giving positive results and two animals giving
negative results were slaughtered from each group and
confirmation of infection was ascertained by histopathology.
Fecal samples and rectal pinch from all the animals were
subjected to Zeihl-Neelsen's acid-fast staining for detection
for M. paratuberculosis. Serum magnesium levels of all testpositive
animals and an equal number of test-negative animals
from each group were compared.
After 24 h MID-I was found to be superior to both SID
and MID-II tests. The MID-I test had higher specificity (66.6
per cent against 58.33 per cent in SID and 40 per cent in
l^iiD-ii) and predictive value of positive results (83.3 against
55.5 in SID and 50 per cent in MID-II)• The MID-I and MID II
tests had 100 per cent sensitivity and predictive value of
negative results as against 50 per cent and 33.3 per cent of
SID.
Examination of fecal sample and rectal pinch was found
to be of little value in the diagnosis of early
paratuberculosis in goats.
Serum magnesium levels between test positive and test
negative animals was found to be not significantly different
in all three groups.
The results of the present study indicate that MID-I
test could be used as an efficient diagnostic test for
detection of paratuberculosis among goats.
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