Thumbnail Image

Kerala Veterinary and Animal Sciences University, Wayanad

Browse

20211
Show more
Reset filters
Subject: Clinical Veterinary Medicine, Ethics and Jurisprudence × Author: ANAZ S.R. × Type: Thesis ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    SUITABILITY OF SALINE-ADENINE-GLUCOSE MANNITOL ADDITIVE IN CITRATE-PHOSPHATE DEXTROSE-ADENINE FOR STORING PACKED RED BLOOD CELLS OF GOATS
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2021-01-21) ANAZ S.R.; N. Madhavan Unny
    In veterinary transfusion medicine, studies related to farm animal transfusions are scarce. Due to the rise in emotional and commercial value, need of transfusion in this area is increasing day by day. The present study entitled ‘Suitability of saline-adenine glucose-mannitol additive in citrate-phosphate-dextrose-adenine for storing packed red blood cells of goats’ envisages the identification of a suitable storage media for goat blood upto 42 days. Ten apparently healthy goats weighing 35 to 60 kg within an age range of 4 to 8 years maintained at University Goat and Sheep Farm, Mannuthy and other organized farms in Thrissur district were selected for the study. Whole blood units were collected from the selected animals using commercially available CPDA-SAGM blood bags under aseptic conditions. After centrifugation of each unit (350ml), plasma was separated and SAGM were added to pRBCs and stored at 4 ± 20C in dedicated refrigerator upto 42 days. For analysing the parameters, samples were collected from the bag every two weeks from the day of collection to day 42. Light and scanning electron microscopic (SEM) studies were carried out on day 0, 28 and 42 of storage. Biochemical and haematological parameters, RBC haemolysis assessment, morphological variation and cultural studies of stored blood was carried out. The pH of the stored blood remained stable whereas glucose showed a significant reduction. Potassium, malondialdehyde and reduced glutathione increased throughout the storage. Haemoglobin and volume of packed red cells did not show any significant change during storage. Haemolysis of RBCs was analysed using osmotic fragility test. The mean cell fragility of pRBCs did not show a major change from day 14 to 42 of storage. Anisocytosis and poikilocytosis was recorded in light microscopy. In SEM only a few acanthocytes and dacrocytes were recorded. Storage related variations were recorded in the samples as the storage period progressed. The study suggests that CPDA-SAGM is a suitable storage media for caprine pRBCs.