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Kerala Veterinary and Animal Sciences University, Wayanad

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2016 - 20184
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Subject: Animal Reproduction, Gynaecology and Obstetrics × End date: 2019 × Start date: 2010 × Thesis Type: Ph.D ×
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Now showing 1 - 4 of 4
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    ThesisItemOpen Access
    YIELD AND QUALITY OF TRANSVAGINALLY RETRIEVED OOCYTES IN NORMAL AND REPEAT BREEDING CATTLE
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2017-12-01) ABHILASH R. S; ABHILASH R. S; Metilda Joseph; Metilda Joseph
    The study was conducted to assess the efficacy of transvaginal oocyte recovery (TVOR) and to evaluate the quality of oocytes recovered from 12 normal and 12 repeat breeder crossbred cows stationed at the University Livestock Farm and Fodder Research Station, Mannuthy. Both normal and repeat breeder cows further divided into two groups of six animals each based on the TVOR frequency. Transvaginal oocyte recovery was performed at a frequency of once weekly in groups I and III and twice weekly in group II and IV for a period of two months (Group I and II- normal cows, and group III and IV- repeat breeders, six animals each). Frequency of TVOR or reproductive status of animal was not found to influence ovarian biometry. Number of small ( 9 mm) follicles were significantly lower (p ≤ 0.05) in normal and repeat breeder cows subjected to twice weekly TVOR. The number of follicles aspirated, number of oocytes retrieved, culture grade oocytes, matured oocytes, fertilized oocytes, cleaved oocytes per session and oocyte recovery rate (%) were siginicantly higher (p ≤ 0.05) in normal animals and those animals subjected to twice weekly TVOR. However, there was no significant difference between maturation rate, fertilization rate and cleavage rates between normal and repeat breeder animals and animals subjected to once and twice weekly TVOR. Evaluation of oocyte maturation rate using Hoechst 33342 and FDA also revealed that maturation rate was higher in normal breeders. No significant difference in the serum progesterone and blood urea nitrogen level were observed when normal and repeat breeder cows were subjected to TVOR at varying frequencies. A significantly higher follicular fluid progesterone concentration was observed in normal animals when compared to repeat breeders. The present study revealed that oocyte quality in repeat breeders is significantly lower than normal breeders, and TVOR at twice weekly interval was found to be an effective tool for harvesting maximum number of oocytes from crossbred cows.
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    ThesisItemOpen Access
    DIAGNOSIS AND THERAPEUTIC MANAGEMENT OF CANINE PYOMETRA FOR RESTORING BREEDING EFFICIENCY
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES, MANNUTHY, THRISSUR, 2018-05-09) UNNIKRISHNAN M. P.; M.O.Kurien
    A study was undertaken to evaluate the efficacy of different therapeutic protocols in the treatment of canine pyometra, based on clinical, haematobiochemical, B-mode ultrasonographic and Doppler ultrasonographic evaluation. Combination treatment using mifepristone @ 2.5mg/kg b. wt. bid orally for five days, along with incremental doses of dinoprost from 10 to 50 µg/kg b. wt. tid; 48 h after initiation of mifepristone, till complete uterine evacuation was superior for therapeutic management of canine pyometra in terms of clinical recovery and future fertility. Haematology recorded anaemia, leucocytosis with neutrophilia and lymphopenia in patients with pyometra, which resolved after treatment. Serum biochemistry revealed marginal rise in blood urea nitrogen, high C-reactive protein and rise in progesterone values, which reduced to normal by day 15 of treatment. Early reduction in progesterone was noticed in cabergoline-based group. B-mode ultrasonography revealed uterine horn distension with anechoic to hypoechoic contents and thickened wall, which reduced to normal by day 15 of treatment. Delay in uterine resolution was noticed in cabergoline-based group. Doppler ultrasonography revealed increased uterine blood flow velocity (peak systolic velocity: 0.69 ± 0.05 to 0.75 ± 0.07; end diastolic velocity: 0.27 ± 0.02 to 0.31 ± 0.01) and decreased resistive index (0.59 ± 0.04 to 0.62 ± 0.06), which returned to normal after treatment. Bacteriological isolates from anterior vagina revealed E. coli (52.63 %), followed by Staphylococcus spp. (37.74 %), Streptococcus spp. (5.66 %) and Pseudomonas (3.77 %). Escherichia coli and Pseudomonas spp. were sensitive to amikacin, whereas Staphylococcus spp was most sensitive to ceftriaxone-tazobactam. Streptococcus spp. was 100 per cent sensitive to cephalosporin. Hyper-salivation was the most common side effect of prostaglandin treatment, exhibited in 31.25 per cent of dogs. Time taken for complete uterine evacuation varied between 5.88 ± 0.40 and 8.25 ± 0.73 days, with more in cabergoline-based group. Fertility studies revealed better cyclicity and conception in 75 and 66.67 per cent, respectively of mifepristone-dinoprost combination treated dogs, whereas overall cyclicity and conception was 68.75 and 54.55 per cent, respectively. Overall recurrence of 28.13 per cent was recorded after medical treatment with minimum recurrence (12.5 %) in mifepristone-dinoprost treated dogs.
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    ThesisItemOpen Access
    CHARACTERIZATION OF MALABARI BUCK SEMINAL PLASMA PROTEINS IN RELATION TO SEMEN FREEZABILITY AND FERTILITY
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCESMANNUTHY, THRISSUR, 2016-12-30) AMBILI JOHN; Hiron M. Harshan
    The study was undertaken to characterize the protein profile of Malabari buck seminal plasma for identifying protein markers of freezability and fertility and to compare the in vivo fertility of Malabari buck semen with low and high semen freezability. Adult Malabari bucks with post thaw sperm progressive motility of more than 35 per cent were classified as having high semen freezability while those with less than 30 per cent were classified as having low semen freezability (four each) were utilized for the study. Tris - egg yolk based extender was used for cryopreservation of the ejaculates (n = 48; 24 each from bucks having high and low semen freezability). Significantly higher values were noted in the pre-freeze acrosome integrity and membrane cholesterol levels and post-thaw motility, viability, acrosome integrity, hypo osmotic sperm swelling response (HOS) and membrane cholesterol levels of high semen freezability bucks when compared with low semen freezability bucks. Whereas the seminal plasma ALP and LDH were found to be significantly lower in high semen freezability bucks compared with low semen freezability bucks at post thaw stage. The total protein content and average number of protein bands in seminal plasma of high and low semen freezability bucks did not differ significantly (75.82 ± 4.62 mg/ml vs 67.07 ± 6.07 mg/ml; 20.83 ± 0.53 vs 22.09 ± 0.49). The 24 kDa, 79 kDa, 84 kDa and 134 kDa proteins were found to have significantly higher occurence in seminal plasma of bucks with high semen freezability whereas the occurence of 15 kDa, 19.9 kDa, 21.5 kDa, 70kDa and 89 kDa proteins were found significantly higher in seminal plasma of bucks with low semen freezability. On analysis of 2D gels, an average of 67.67 ± 4.06 and 75.33 ± 4.28 spots (pI 3-10) were detected in semen of high and low semen freezability bucks, respectively and the difference was not significant. On western blot analysis, osteopontin, a protein fertility marker, was found to be present in seminal plasma of all the bucks studied. For fertility studies 134 does were inseminated with semen preserved by chilling and 91 with cryopreserved semen. The conception rate (CR) with cryopreserved semen was significantly higher in bucks with high semen freezability than with low semen freezability. In the semen preserved by chilling, no significant difference was noticed between low and high semen freezability animals in the spermatozoa progressive motility, viability, acrosome integrity, HOS response or seminal plasma ALP and LDH levels after dilution or after 24 h of storage at refrigeration. But when chilled semen was used, the CR was significantly higher for bucks with low semen freezability than for bucks with high semen freezability.
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    ThesisItemOpen Access
    AUGMENTING REPRODUCTIVE EFFICIENCY IN POST PARTURIENT DOES WITH INTRAVAGINAL PROGESTERONE SPONGES, PROSTAGLANDIN F2α AND GONADOTROPIN RELEASING HORMONE
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2016-12-30) UPASANA RATNAKARAN; K.N. Aravinda Ghosh