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Anand Agricultural University, Anand
Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur
AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.
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ThesisItem Open Access ASSESSMENT OF GENETIC VARIABILITY AND CHARACTER ASSOCIATION STUDIES IN Aloe (Aloe barbadensis Mill.) FOR AGRO - BOTANICAL AND QUALITY TRAITS(AAU, Anand, 2004) DEORE, ABHIJIT A.; Sriram, S.Aloe (Aloe barbadensis Mill.), one of the priority medicinal plants of commercial importance in India, belongs to the family Liliaceae. Dry weight of juice and aloin content (gm/100 gm dry exudate) are important economic traits of this plant. These traits are manifestations of many contributing characters. As the work on genetics of these traits is scarce in the literature, the present investigation was undertaken. Information was generated on variability, character associations as well as direct and indirect effects of different characters on these economic parameters of Aloe. The field experiment was conducted during January-2002 to February-2003 at the experimental farm of Medicinal and Aromatic Plants Project, Anand Agricultural University, Anand. The experimental material (19 months old) comprised of 23 genotypes. The evaluations were made in completely randomized design. Observations were recorded on randomly selected five plants and each plant was treated as single repeat. Analysis of variance revealed significant differences among the genotypes for all 35 characters studied suggesting presence of good deal of variability in the experimental material. The characters studied were length of inflorescence, number of flowers per inflorescence, density of flowers in an inflorescence, per cent pollen fertility, number of capsules per inflorescence, number of seeds per inflorescence, number of seeds per capsule, per cent seed germination, leaf length, leaf breadth, leaf thickness at the base, number of leaves per plant, weight of single leaf, weight of gel, volume of gel, dry gel content (gm/100 ml juice), fresh weight of juice, dry weight of juice, moisture per cent in juice, fresh weight of exudate, dry weight of exudate, moisture per cent in exudate, dry exudate content (gm/100 ml exudate), cold extracted gel (gm/100 ml juice), cold extracted gel (gm/100 gm gel powder), hot extracted gel (gm/100 ml juice), hot extracted gel (gm/100 gm gel powder), aloin content (gm/100 gm fresh exudate), aloin content (gm/100 gm dry exudate), aloin content (gm/100 gm fresh juice), aloin content (gm/100 gm gel powder), barbaloin content (gm/100 gm fresh exudate), barbaloin content (gm/100 gm dry exudate), barbaloin content (gm/100 gm fresh juice) and barbaloin content (gm/100 gm gel powder). Characters, length of inflorescence, number of flowers per inflorescence, density of flowers, per cent pollen fertility, number of seeds per inflorescence, per cent seed germination, weight of leaf, weight of gel, volume of gel, fresh weight of juice, dry weight of juice, fresh weight of exudate, dry weight of exudate, cold and hot extracted gel in juice, cold and hot extracted gel in powder showed predominance of genotypic variance and also showed high magnitude of broad sense heritability coupled with high genetic advance. Hence, it is concluded that experimental material possessed potential for improvement in these traits. Correlation matrix revealed that all character paired were positively and significantly correlated with each other both at genotypic and phenotypic levels but for number of flowers per inflorescence and number of leaves per plant which were negatively correlated. Characters, length of inflorescence, number of flowers per inflorescence, leaf length, leaf breadth, leaf thickness at the base, weight of single leaf, weight of gel and fresh weight of juice showed their highly significant and high magnitude positive association with both the economic parameters viz., dry weight of juice and aloin content (gm/100 gm dry exudate). Hence, based on high magnitude correlation coefficient with economically important characters, it could be concluded that certain inflorescence and leaf characters could be exploited for improvement in this crop. Path analyses revealed that leaf thickness at the base and weight of gel had positive direct effect on both the economic parameters i.e. dry weight of juice and aloin content (gm/100 gm dry exudate). Other characters, length of inflorescence, number of flowers per inflorescence, leaf length, leaf breadth, weight of single leaf and fresh weight of juice had direct but negative effect on these economic traits. Though their correlation with economic traits was positive and significant, their direct and indirect effect on both the economic parameters was variable. In general, characters leaf thickness at the base and weight of gel, with high magnitude of direct effects may be helpful to select the elite genotypes for improvement in these two economic traits i.e. selection could be based on visibly assessable character leaf thickness at the base and later results could be confirmed in laboratory, testing for gel weight. Based on these analyses and mean values, eight elite genotypes viz., IC-112522, IC-112527, KM 12535, IC-112528, KM 11269, KM 12531, Bitter rayan and Sweet rayan were identified for further use in crop improvement programme.ThesisItem Open Access STUDIES ON SOME ASPECTS OF INFERTILITY IN JERSEY COWS USED EXTENSIVELY IN EMBRYO TRANSFER TECHNOLOGY.(AAU, Anand, 2004) SHAH, RAKHIBEN MADANBHAI; PATEL, D. M.The present investigation on "Studies on some aspects of infertility in Jersey cows used extensively in Embryo Transfer Technology" was undertaken on Jersey animals (n=10) at the Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand Campus Anand. The study was carried out during the months of May 2003 to September 2003 The experimental animals were located at Reproductive Biology Research Unit, Veterinary College, Anand. All the Jersey cows were used in research related to non-surgical embryo transfer and were super-ovulated and flushed number of times under the strict Veterinary care. Also, excellent quality embryos after evaluation were transferred into some these animals, which served as recipients. Preliminary examination was made to know the reproductive status of the animals. Animals were divided in two groups. In first group normal estrus cycle of animals before breeding were observed. In the second estrous cycle of the first group all the animals were given intrauterine antibiotic, ampicilin and cloxacilin preparation (Ampoxin 2 gm containing ampicilin 1000 mg. and cloxacilin 1000 mg). In the second group animals were treated with GnRH (Receptal, 5 ml, I/M) and were bred. Blood collection was made at weekly interval and the pregnancy diagnosis was done on day 45 post breeding. The blood serum levels of glucose, calcium, phosphorus, calcium: phosphorus ratio, iron, copper, cobalt, zinc, manganese were lower in these animals. Repeated rectal examination of these cows revealed the cause of infertility to be cystic ovarian degeneration (two animals), ovarobursal adhesion (one animal), and early embryonic mortality (two animals). Tubal insufflation method of testing fallopian tube patency revealed bilateral complete tubal blockage in two animals and partial tubal blockage in three animals. These findings clearly demonstrated that superovulation in embryo transfer technology lowers the fertility in cows and repeated super ovulation lead to sterility in cows.ThesisItem Open Access GENETIC ARCHITECTURE OF GREEN FRUIT YIELD AND QUALITY CHARACTERS IN RELATION TO GMS BASED HYBRIDS IN CHILLI (Capsicum annuum var. longum (D.C.)Sendt.)(AAU, Anand, 2004) PATEL, PRAGNESH R.; Patel, J. A.The present investigation comprised of 40 F1S developed from four Gms lines and ten male parents through Line x Tester mating design in chilli (Capsicum annuum var. longum (D.C.) Sendt.) was undertaken at Main Vegetable Research Station, Gujarat Agricultural University Anand during kharif planting crop season of the year 2002-03. The experimental material (40 F1S, 4 Gms lines, 10 pollen parents and one check variety) was evaluated in complete randomized block design with three replications. The main objective of the experiment was to study heterosis, gene effects and combining ability effects for green fruit yield and various yield as well as quality characters. Analysis of variance indicated presence of considerable amount of variability among genotypes for all the fourteen characters studied. Mean squares due to parents and hybrids were significant for all the characters. Whereas, parents was. hybrids contrast comparison was significant for all the attributes except average fruit weight, suggesting the existence of differences between parents and hybrids leading to evidence for presence of heterosis for all those characters. In respect to per se performance of parents, among the Gms lines ACMS-3 and ACMS-4 and among the male inbreds ACS 97-2, ACS 2000-01. ACS 2000-02, ACS 2000-03, Punjab Gucchedar and RHRC Pendent were found to be superior for green fruit yield and most of the yield as well as quality characters. Among the hybrids. ACMS-2 x Pb. Gucchedar. ACMS-3 x Pb. Gucchedar. ACMS-3 x ACS 97-2, ACMS-4 x Arka lohit and ACMS-2 x ACS 2000-02 had high per se mean for green fruit yield. These hybrids also possessed high mean values for other important characters in respect to yield and quality. In the present investigation, significant and higher estimates of relative heterosis, hetcrobeltiosis and standard heterosis were observed for green fruit yield. The hybrids ACMS-2 x Pb. Gucchedar. ACMS-4 x ACS 2000-03, ACMS-4 x ACS 2000-01 and ACMS-3 x Pb. Gucchedar exerted greater amount of heterobelliosis and standard heterosis for yield, these hybrids also depicted larger estimates of heterosis for number of fruits per plain. These lop ranking hybrids also registered significant desirable heterosis for most of the growth and quality characters in addition to important yield contributing component characters. For number of fruits per plant, hybrid ACMS-3 x Pb. Gucchedar borne the highest fruits followed by ACMS-4 x Arka lohit and ACMS-2 X Pb. Gucchedar. likewise these hybrids also depicted the maximum values of standard heterosis and larger estimates of heterobeltiosis for the said trait. These hybrids also registered higher estimates of various heterotic effects for one or more than one yield contributing component characters. In respect to earliness, hybrids ACMS-: X ACS 2000-03. ACMS-4 x ACS 2000-03 and ACMS-1 x ACS 96-33 look the minimum days for flowering. hence these hybrid had the least estimates of standard heterosis. The estimates of components of genetic variance revealed the importance of non additive gene effect for genetic control of green fruit yield and its important component character number of fruits per plant, for all the growth attributes except plant height and for all the quality parameters. Additive gene effect was importance for fruit length and fruit diameter. Whereas, both the components of genetic variance were significant for average fruit weight. For plant height and fruit shape index none of the gene effects had significant estimates.ThesisItem Open Access LINE X TESTER ANALYSIS FOR COMBINING ABILITY, HETEROSIS AND GENE ACTION IN OKRA (Abelmoschus esculentus (L.) Moench(AAU, Anand, 2004) AHLAWAT, TIMUR RAMPAL; Joshi, K. I.The present investigation entitled "Line x Tester analysis for combining ability, heterosis and gene action in okra (Ahelmoschus esculentus (L.) Moench)" was undertaken at Main Vegetable Research Station, Gujarat Agricultural University, Anand during kharif 2000 and 2001. The experimental material comprising of fifteen females, five males, their 75 hybrids and standard hybrid check GOH-1 was evaluated in a randomized block design with three replications in both the environments. Observations were recorded on fruit yield per plant and eight other yield components. The individual environment-wise and pooled analysis of variance revealed sufficient variation among the genotypes for all the traits studied, indicating the existence of sufficient variability in the material. The pooled analysis over environments revealed that environmental differences were non-significant for all the characters, thereby suggesting that the characters were not influenced by the changes in environment. Considerable level of heterosis was observed for fruit yield and its component traits. Maximum heterosis was observed for number of primary branches per plant followed by fruit yield per plant, plant height, fruits per plant and fruit weight. However, the magnitude of heterosis varied in different environments for the various traits studied.ThesisItem Open Access INTEGRATED NUTRIENT MANAGEMENT IN Kharif PIGEONPEA [Cajanus cajan (L.) Millsp.] UNDER IRRIGATED CONDITION(AAU, Anand, 2004) PATEL, CHIRAGKUMAR NATVARBHAI; SADHU, A. C.A field experiment was conducted during Kharif season of 2002 at the College Agronomy Farm, Gujarat Agricultural University, Anand campus, Anand to study the "Integrated nutrient management in Kharif pigeonpea [Cajanus cajan (L.) Millsp.] under irrigated condition". The soil of the experimental plot was loamy sand in texture having good drainage, low in nitrogen, medium in phosphorus and rich in potash with pH 7.9. Twelve treatment combinations consisting of four levels of bio-fertilizer / FYM (control, Rhizobium inoculation, Rhizobium + PSB inoculation and Rhizobium + PSB inoculation +FYM @ 10 t / ha) and three fertility levels (0 %, 50 % and 100 % recommended dose of fertilizers i.e. 25 kg N + 50 kg P2O5 / ha) were tested under factorial randomized block design with four replications. The results indicated that the growth and yield parameters as well as yield and protein content in grain were significantly affected by different bio-fertilizer / FYM levels. The treatment of Rhizobium + PSB inoculation +FYM @ 10 t / ha recorded significantly the highest plant height at harvest, no. of branches / plant, no of pods / plant, pod length and test weight. This treatment being at par with Rhizobium + PSB inoculation treatment also recorded significantly higher values of plant height at 30 DAS, no of seeds / pod, grain yield / plant and protein content in grain (23.59 %) than control and Rhizobiiim inoculation alone. Marked increase in the growth and yield attributes resulted in significantly the highest grain and straw yields (1495 kg/ha and 5186 kg/ha, respectively) under the treatment of Rhizobium + PSB inoculation +FYM @ 10 t / ha. It also recorded the highest net realization of Rs 16,413 / ha, while maximum C.B.R. of 3.35 was recorded under the treatment of Rhizobium + PSB inoculation. The results revealed that different fertility levels had marked influence on various parameters except harvest index. Each increase in fertility levels from no. fertilizer to 100 % recommended fertilizer dose (25 kg N + 50 kg P2O5 / ha) significantly increased the growth and yield attributes, yield as well as protein content in grains. Application of 100 % recommended dose of fertilizers recorded significantly the highest plant height at 30 DAS and at harvest, no. branches / plant, no of pods / plant, pod length, no of seeds / pod, test weight, grain yield / plant and this resulted in significantly the highest grain as well as straw yields (1530 kg/ha and 4529 kg/ha, respectively) under this fertility level. Significantly the highest protein content in grain (23.91 %) was also recorded under 100 % recommended fertilizer dose. This treatment also gave the highest net realization of Rs 17,462 / ha with maximum C.B.R. of 3.12. During the course of investigation, interaction effect of bio-fertilizer / FYM levels X fertility levels revealed that treatment combination of Rhizobium +PSB inoculation +FYM @ 10 t / ha +100 % recommended fertilizer dose (25 kg N + 50 kg P2O5 / ha) recorded the highest no.of pods / plant (147.8) and the highest grain yield (1643 kg/ha). Rhizobium +PSB inoculation +FYM @ 10 t / ha + 100 % recommended fertilizer dose also recorded the highest net realization of Rs 17490 / ha, while the maximum C.B.R. (3.12) was recorded under treatment combination Rhizobium +PSB inoculation +100 % recommended fertilizer dose (25 kg N + 50 kg P2O5 / ha). In light of the results obtained from this investigation, it is revealed that the seeds of pigeonpea should be treated with Rhizobium and Phosphorus solubilizing bacteria along with the application of 10 t FYM / ha and 100 % recommended fertilizer dose (25 kg N + 50 kg P2O5 / ha) for getting maximum yield and net profit from pigeonpea under middle Gujarat agro-climatic conditions.ThesisItem Open Access STUDIES ON CLINICO-ETIOPATHOLOGY AND THERAPEUTIC MANAGEMENT OF VARIOUS CANINE DERMATOSES(AAU, Anand, 2004) Nair, Sreegeetha Sreedharan; Nauriyal, D. S.On screening of 2618 dogs brought at the Zaveri Clinic affiliated to the College of Veterinary Science and Animal Husbandry, Anand during the period January 2001 and December 2001, the overall incidence of dermatitis was observed to be 23.68 per cent (620 cases). Of the various types of dermatitis studied the incidence of tick infestation was found to be maximum (19.19%) whereas that of acanthosis nigricans was minimum (0.32 per cent), Greater number of cases of dermatitis were observed during the rainy season (45.48%) while breedwise GSD (27.90%), Spitz (26.12%) and Mongrel (21.29%) showed higher susceptibility to skin afflictions. More than 50 per cent of the affected dogs were found to be males and 48.06 per cent dogs presented with dermatological afflictions were between 1 to 6 years of age. Microscopic examination of the skin scrapings collected from suspected cases of scabies, demodicosis and fungal infection revealed Demodex spp. (10 cases), Sarcoptes scahiei var. cams (13 cases) and fungal hyphae or spores (15 cases). Further, cultural inoculation of scrapings from suspected cases of fungal infection resulted in isolation of 10 fungal isolcates identified as Trichophyton spp. (2), filamentous fungi (4), Aspergilhis spp. (2) and yeast (2 isolates). The bacteriologjcal culture examination of 43 pus swabs collected from pyogenic skin lesions resulted in the growth of 44 bacterial isolates which were obtained either as nionomicrobic or as mixed culture. Similarly bacterial cultural examination of otic exudates also yielded growth of bacterial isolates in pure culture or mixed culture, Among various bacteria cultured, Staphylococcus spp, accounted for the highest number of isolates from cases of pyoderma as well as otitis externa. One of the ear exudates collected from a Cocker Spaniel with seborrhoeic sicca resulted in the isolation of Malassezia pachydcnnatis: In vitro antibacterial susceptibility test performed on all the bacterial isolates obtained from cases of pyoderma and otitis externa exhibited highest sensitivity to ciprofloxacin which also proved to be the most effective antibacterial when used in the treatment of clinical cases. Hematological study conducted on dermatitis revealed statistically significant decrease in hemoglobin concentration and total erythrocytic count, increased erythrocyte sedimentation rate, neutrophilia and eosinophilia, Histopathological study of the biopsy specimens collected from cases of dermatological afflictions revealed characteristic changes. The histopathological alterations observed in the biopsy specimens of pyoderma comprised of acute superficial pyoderma, deep pyoderma and chronic suppurative deimatitis. Periodic Acid Schiff (PAS) stained sections of skin infected with fungi showed fungal spores and hyphae along with other pronounced histological changes such as hyperkeratosis, acanthosis, presence of scales on the epidermis as well as follicular changes. In case of scabies, predominenl changes noticed included presence of degenerated pieces of mites mixed with exudate in the epidermis or epidermal burrows, in demodicosis, the predominent changes included dilatation of hair follicles with mites, folliculitis and destruction of hair follicles and dermal tissue. Skin biopsies from cases of flea-allergic dermatitis, tick, lice and fly infestations showed body parts of parasites and their excreta mixed with keratin and cellular debris on the superficial epidermis. The cases of allergic dermatitis revealed epidermal and dermal oedema, superficial exudate, necrotic changes and cellular infiltration. The seborrhoeic lesions revealed hyperkeratosis of epidermis, hyperkeratotic projection of papillae and keratin plugging of the hair follicles. In acral lick dermatitis, notable microscopic changes included sebaceous gland hyperplasia, hyperkeratinization and destruction of collagen fibre. The biopsy from callus lesions revealed subepidermal cyst and keratin pearl and dermal fibrosis. Histological changes in acanthosis nigrican included detachment of keratin layer, epidermal papillae and atrophy of hair follicles. Histopathological investigation of tumourous masses revealed characteristic lesions of lipoma, fibromelanoma, hemangioma and adenocarcinoma. The cases of pyoderma and pyogenic lesions were treated successfully with systemic antibiotics like ciprofloxacin, erythromycin and cephalexin and topical antiseptic preparations. The dogs suffering from fungal infections were treated effectively with topical antifungal drugs alone or in combination with griseofulvin. In infestations caused by Sarcoptes and Demodex spp., topical use of amitraz and subcutaneous injection of ivermectin or simultaneous use of both drugs brought about clinical and parasitological cure. In case of scabies, use of other ectoparasiticidal drugs viz. deltamethrin and cypermethrin also proved to be effective. In flea-infested dogs and dogs with other arthropod parasites like ticks, lice and flies, treatment of both the animal and its environment with acaricidal drugs was found to be effective in controlling parasite population. The ectoparasiticidal compounds found effective against fleas were carbaryl and deltamethrin. The insecticides found effective against ticks, lice and flies included deltamethrin, cypermethrin, carbaryl and amitraz. Combination of topical use of these drugs along with subcutaneous injection of invermectin also proved to have appreciable parasiticidal effects on arthropods. The effective management of immune mediated dermatoses (contact dermatitis, atopy, food allergy and drug allergy) was done by identification of the underlying cause, its correction and control of pruritus with topical and systemic antipruritic agents. In cases of seborrhoea, the lesions showed resolution with the use of selenium sulphide containing antiseborrhoeic shampoo for bathing, topical use of keratolytic compound along with dietary supplementation with sunflower or peanut oil. The cases of acral lick dermatitis were treated effectively with combination of drugs comprising of oral administration of corticosteroids and intra-lesional injections of triamcinolone acetonide. The callus condition was clinically managed by daily soaking of the affected area in luke-warm water and topical use oremollienl cream on the lesions. The lesions observed in acanthosis nigrican were treated with topical application of compound containing benzoic acid and use of corticosteroid drug. The pruritus associated with various disorders barring demodicosis was controlled with systemic use of prednisolone in tapering dosage. Non-sleroidal antiinflammatory drugs like clemastine and hydroxyzine also showed excellent therapeutic response in most of the cases of pruritus. The treatment protocol found effective in the treatment of ear infection comprised of cleaning of ear debris using a ceruminolytic/ear wax dissolvent and subsequently instillation of ear preparations containing antibacterial drugs (in cases of bacterial infection) or antifungal drugs (in case of mycotic infection). The cases of otitis caused by Malassezia pachydermatis were treated successfully by systemic use of ketoconazole and instillation with otic preparation containing antifungal agent.ThesisItem Open Access MOLECULAR CHARACTERIZATION OF "KATHIAWARI" BREED OF HORSE USING MICROSATELLITE MARKERS(AAU, Anand, 2004) GANGARAMBHAI, KORINGA PRAKASHKUMAR; Solanki, J. V.It is essential to characterize the germplasm for introgenetic variability, which will help in planning for conservation strategy as well as genetic improvement. Microsatellite markers are widely accepted as a marker of choice as they are highly polymorphic single locus DNA sequenced throughout the genome and are readily adaptable to Polymerase Chain Reaction (PCR). Present study is an attempt to characterize Kathiawari horse breed using fifteen sets of equine microsatellite markers. DNA samples were taken from sixty horses from its native tract. These samples were assessed for genetic variation using fifteen equine specific microsatellite markers viz. loci NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15. All samples did not respond to PCR amplification. Forty seven to fifty eight samples yielded amplification. The PCR products were checked on 2% agarose for amplification. Further the amplicons wefe run on sequencing PAGE (DenaturingPAGE) with 10 bp ladder for allele sizing and allele typing. The sizes (length) of different microsateliites were found to be approximately 153bp, 174bp, 173bp, 176bp, 216bp, 142bp, 122bp,200bp,137bp, 166bp,178bp, 126bp, 97bp, 142bp, and 144bp for microsateliites NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15, respectively. A wide range of genetic analyses were then performed on the resultant data. A high level of genetic variation was observed. Microsateliites NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15 showed 4, 4, 2, 5, 6, 4, 5, 5, 8, 8, 6, 6, 8, 4 and 3 alleles as well as 7, 7, 2, 7, 13, 6, 12, 9, 11, 20, 14, 10, 20, 9 and 5 genotypic combinations, respectively. The heterozygosity values in these microsateliites were 0.5306, 0.5745, 0.1400, 0.6400, 0.8600, 0.5472, 0.8200, 0.8200, 0.6792, 0.7959, 0.7241, 0.8478, 0.8235, 0.7400 and 0.5185 for microsateliites NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15 respectively. Microsatellite LEX 20 was highly polymorphic and NVHEQ 54 was least polymorphic among all fifteen microsateliites. Polymorphic information content (PIC) was calculated from number of alleles and heterozygosity. It was 0.441, 0.485, 0.122, 0.483, 0.737, 0.434, 0.732, 0.664, 0.711, 0.752, 0.707, 0.699, 0.820, 0.682 and 0.377 for microsatellite NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15 respectively. The microsatellites were found to be highly polymorphic with 2 to 8 alleles, 0.1400 to 0.8600 heterozygosity and 0.122 to 0.820 PIC in the Kathiawari horse. This is the first reported molecular characterization study on Kathiawari horse.ThesisItem Open Access BIOCHEMICAL CHARACTERIZATION, ANTIMICROBIAL SENSITIVITY, PCR - BASED DETECTION AND MOUSE PATHOGENICITY OF PASTEURELLA MULTOCIDA FIELD ISOLATES(AAU, Anand, 2004) KIRTIBHAI, PATEL HETALBEN; Purohit, J. H.Two isolates of Pasteurella multocida each from poultry, sheep, rabbit, buffalo and the vaccine strain (P52 strain) were studied for cultural, morphological and biochemical characterization, antimicrobial sensitivity, PCR-based detection and mouse pathogenicity of P. multocida field isolates. The heart blood and organs of the experimental mice were processed for reisolation of P. multocida. The heart blood and reisolated organisms from the experimental mice were subjected to PCRbased detection. All the test isolates were Gram negative, cocco-bacillary rods and produced non-haemolytic colonies on blood agar and failed to grow on MacConkey agar. All the isolates (100%) were positive for oxidase, catalase, indole production, nitrate reduction and fermentations of glucose, mannitol, sucrose and mannose while negative for citrate utilization and fermentation of maltose, arabinose, lactose, dulcitol, salicin and trehalose. The isolates (100%) were sensitive to enrofloxacin, flumequine, chloramphenicol and tetracycline, while nine isolates (90%) were sensitive to norfloxacin and cephalexin, two isolates (20%) were sensitive to penicillin G. All the isolates were (100%) resistant against sulphadiazine while four isolates(40%) were intermediate to penicillin G. Sixty per cent of P. multocida isolates were sensitive to Allium sativum, 40% sensitive to Ocimum sanctum, 20% sensitive to Zingiber officinale, 10% sensitive to Azadirachta indica, 10% sensitive to Curcuma longa. All the isolates were lethal to mice. Significant difference was observed in death time between species and between dilutions. Poultry isolates were found highly pathogenic to mice. Gross changes in mice were characterized by congestion and haemorrhages in most of vital organs. Histopathological lesions were characterized by mild to sever congestion, haemorrhages, and bacterial emboli. PM-PCR from reisolated colonies of P. multocida and gemomic DNA of test isolates were tested by PCR-based characterization, which revaled amplified product of approximately 465 bp size of all the P. multocida isolates, E.coli did not show amplification. Direct PCR from mice blood could not show any amplification.ThesisItem Open Access SURVEILLANCE OF HAEMORRHAGIC SEPTICAEMIA IN GUJARAT STATE WITH ISOLATION, BIOCHEMICAL CHARACTERIZATION AND PCR BASED DETECTION OF PASTEURELLA MULTOCIDA FROM THE FIELD OUTBREAKS(AAU, Anand, 2004) NADODHA, J. V.; Purohit, J. H.The present research work was undertaken with a view to study the surveillance of Haemorrhagic Septicaemia in relation to agro-climatic zones of Guajrat State during the period of January 1998 to December 2003 with isolation and identification of Pasteurella multocida from the field outbreaks in cattle and buffaloes. The isolates were studied for their in vitro antibiotic sensitivity. The PM-PCR was also tried for detection of P. multocida. The data during the study period were collected from the respective sources. The data were compiled and distributed year, month and zonewise. Incidences of HS were correlated to meteorological parameters of different zones. A total of 226 clinical samples (109 each of blood and nasal swabs and eight morbid material) were collected from suspected cases of HS in cattle and buffaloes and processed for demonstration of bipolar organisms and isolation of P. multocida. The isolates were identified by cultural, morphological and biochemical characteristics. The isolates were subjected to in vitro antibiotic sensitivity. The colony PCR was carried out for detection of P.multocida. The highest number of HS outbreaks were recorded during the year 1998 and lowest were found in 2000. The outbreaks were observed throughout the year but more number of outbreaks were found during the rainy season, i.e., in the months of August and September. Amongst the agro-climatic zones, maximum number of outbreaks were recorded in Zone-IV, whereas minimum in Zone-VII. Positive correlation was observed between outbreaks of HS and rainfall, relative humidity and minimum temperature. While no correlation was observed with outbreaks of HS and maximum temperature. A total of four isolates of P.multocida were recovered only from buffaloes blood which showed the presence of bipolar organisms in smear. All the isolates produced non-haemolytic, round, grayish, smooth and mucoid colonies on blood agar but failed to grow on MacConkey agar. The isolates were found non-motile, Gram negative, coccobacillary rods. All the isolates produced oxidase, catalse, indole and reduced nitrate but did not utilise citrate. They fermented glucose, sucrose, mannitol and maimose but not fermented maltose, arabinose, lactose, dulcitol, salicin, inositol and trehalose. All the isolates were found sensitive to gentamicin, chloramphenicol and cephalexin, while two isolates were sensitive to tetracycline, ampicillin and nitrofurantoin. All the isolates were found to be resistant against penicillin-G and streptomycin. All the P. multocida isolates along with P52 vaccine strain amplified product of approximately 465 bp size while E.coli failed to amplify.