SURVEILLANCE OF HAEMORRHAGIC SEPTICAEMIA IN GUJARAT STATE WITH ISOLATION, BIOCHEMICAL CHARACTERIZATION AND PCR BASED DETECTION OF PASTEURELLA MULTOCIDA FROM THE FIELD OUTBREAKS
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Date
2004
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AAU, Anand
Abstract
The present research work was undertaken with a view to study the surveillance of Haemorrhagic Septicaemia in relation to agro-climatic zones of Guajrat State during the period of January 1998 to December 2003 with isolation and identification of Pasteurella multocida from the field outbreaks in cattle and buffaloes. The isolates were studied for their in vitro antibiotic sensitivity. The PM-PCR was also tried for detection of P. multocida. The data during the study period were collected from the respective sources. The data were compiled and distributed year, month and zonewise. Incidences of HS were correlated to meteorological parameters of different zones. A total of 226 clinical samples (109 each of blood and nasal swabs and eight morbid material) were collected from suspected cases of HS in cattle and buffaloes and processed for demonstration of bipolar organisms and isolation of P. multocida. The isolates were identified by cultural, morphological and biochemical characteristics. The isolates were subjected to in vitro antibiotic sensitivity. The colony PCR was carried out for detection of P.multocida. The highest number of HS outbreaks were recorded during the year 1998 and lowest were found in 2000. The outbreaks were observed throughout the year but more number of outbreaks were found during the rainy season, i.e., in the months of August and September. Amongst the agro-climatic zones, maximum number of outbreaks were recorded in Zone-IV, whereas minimum in Zone-VII. Positive correlation was observed between outbreaks of HS and rainfall, relative humidity and minimum temperature. While no correlation was observed with outbreaks of HS and maximum temperature. A total of four isolates of P.multocida were recovered only from buffaloes blood which showed the presence of bipolar organisms in smear. All the isolates produced non-haemolytic, round, grayish, smooth and mucoid colonies on blood agar but failed to grow on MacConkey agar. The isolates were found non-motile, Gram negative, coccobacillary rods. All the isolates produced oxidase, catalse, indole and reduced nitrate but did not utilise citrate. They fermented glucose, sucrose, mannitol and maimose but not fermented maltose, arabinose, lactose, dulcitol, salicin, inositol and trehalose. All the isolates were found sensitive to gentamicin, chloramphenicol and cephalexin, while two isolates were sensitive to tetracycline, ampicillin and nitrofurantoin. All the isolates were found to be resistant against penicillin-G and streptomycin. All the P. multocida isolates along with P52 vaccine strain amplified product of approximately 465 bp size while E.coli failed to amplify.
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VETERINARY MICROBIOLOGY, CHARACTERIZATION