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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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20161
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Subject: Veterinary Pharmacology × Start date: 2014 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    STUDIES ON ANTIDIABETIC EFFECT OF AQUEOUS AND ALCOHOLIC EXTRACTS OF MORINGA OLEIFERA IN STREPTOZOTOCIN INDUCED DIABETIC RATS
    (AAU, Anand, 2016) KARETHA HETALBEN BHIKHALAL; Dr. A. M. Thaker
    The present study was conducted on sixty six (66) male Albino Wistar rats dividing them in various groups having six rats in each group. Group I served as vehicle control and received 0.5 % solution of sodium bicarbonate in normal saline orally once daily for 28 days. Group II served as diabetic control and received streptozotocin at the dose rate of 60 mg/kg body weight, by dissolving it in 50 mM citric buffer (pH 4.5) solution as a single intraperitoneal injection. Rats of group III, IV, V, VI, VII, VIII and IX also received streptozotocin at the same way. Group III received glibenclamide at dose of 5 mg/kg of body weight (p.o.) once daily after establishment of diabetes for 28 days. Group IV, V and VI received aqueous extract of M. oleifera pods at dose of 100 and 200 and 400 mg/kg respectively (p.o.) once daily respectively while group VII, VIII and IX received alcoholic extract of M. oleifera pods at dose of 100 and 200 and 400 mg/kg (p.o.) respectively once daily after establishment of diabetes for 28 days. Whereas group X and XI were administered with aqueous and alcoholic extracts of M. oleifera pods respectively at dose of 200 mg/kg orally once daily for 28 days. Upon acute oral toxicity testing, aqueous and alcoholic extracts of Moringa oleifera pods were found safe. Phytochemical analysis by GC-MS revealed presence of many compounds in both aqueous and alcoholic extracts of pods. Rats of diabetic “Studies on antidiabetic effect of aqueous and alcoholic extracts of Moringa oleifera in streptozotocin induced diabetic rats” control group were found dull and depressed along with polydipsia, polyphagia and polyuria from first week of experiment. At the end of experiment, there was significant reduction in the body weight gain and increased feed consumption was found in diabetic rats which was significantly reversed with administration of standard drug, aqueous and alcoholic extracts of M. oleifera pods. Administration of aqueous and alcoholic extracts of M. oleifera pods at dose rate of 100, 200 and 400 mg/kg body weight and glibenclamide at 5 mg/kg body weight in diabetic rat for 28 days showed significant (p<0.01) reduction in the elevated level of blood glucose and TLC and significant (p<0.01) increase in the reduced level of Hb, RBCs, PCV, MCV, MCH and MCHC in dose- dependent manner. Daily oral administration of glibenclamide at 5 mg/kg body weight and aqueous and alcoholic extracts of M. oleifera pods at dose rate of 100, 200 and 400 mg/kg body weight in diabetic rats for 28 days produced significant (p<0.01) reduction in the elevated level of SGPT, SGOT, TC, LDH, CK and BUN and significant (p<0.01) increase in the reduced level of liver glycogen, albumin and total protein in dose- dependent manner. Microscopic examination of pancreas revealed destruction, decreased number, dearrangement, diminished size and shape of β cells of islets of langerhans and damaged acinar cells, while histopathological examination of pancreas of both extracts and glibenclamide treated groups revealed restoration in damaged histoarchitecture structure. The hypoglycemic effect of glibenclamide, as a reference drug on reducing blood glucose was more potent and significant as compared to plant extracts (aqueous “Studies on antidiabetic effect of aqueous and alcoholic extracts of Moringa oleifera in streptozotocin induced diabetic rats” and alcoholic extracts of M. oleifera pods) treatment and brought all the hematological and biochemical parameters up to the normal level. Aqueous and alcoholic extracts of M. oleifera pods showed effectiveness in dose- dependent manner. Both aqueous and alcoholic extracts of the M. oleifera pods at the dose rate of 400 mg/kg body weight showed better effect than dose rate of 100 and 200 mg/kg body weight. The antidiabetic activity of aqueous and alcoholic extracts of M. oleifera pods may be due to the presence of phytochemical constituents such as quercetin, flavonoids, phenol, glycoside and alkaloids. Further investigation to define its clinical efficacy would be highly desirable.