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    ThesisItemOpen Access
    BACTERIAL AND VIRAL PATHOGENS ASSOCIATED WITH WHITE SHRIMP (Litopenaeus vannamei) CULTURE SYSTEM ALONG KARNATAKA COAST
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2015-07) NARASIMHA MURTHY, Y. G, B.F.Sc; M.N. VENUGOPAL; GIRISHA S.K.; MANJA NAIK B.
    Litopenaeus vannamei is an exotic species recently introduced in India to supplement the failing aquaculture of the Asian tiger shrimp, P. monodon due to viral diseases. After a brief initial success, the culture of L. vannamei is also experiencing serious problems due to bacterial and viral diseases. The present work was aimed at studying bacterial and viral pathogens associated with White shrimp (L. vannamei) culture system along Karnataka coast. A total of 154 Samples consisting of 81 shrimp and 73 farm water samples were collected from shrimp culture ponds along the Karnataka coast and tested for the presence of bacterial pathogens of shrimp (V. parahaemolyticus and V. harveyi) and human pathogens (V. parahaemolyticus, V. cholerae, and Salmonella) and shrimp viral pathogens (DNA and RNA viruses), and confirmation was done by using based molecular approach. Pure isolates of V. parahemolyticus were positive for toxR and tlh genes. V. harveyi and Salmonella isolates were positive respectively for vhh and hnsA genes with low prevalence. Complete absence of V. cholerae was noticed. Except, white spot syndrome virus (WSSV) and infectious hypodermal and haematopoietic necrosis virus (IHHNV), other viral pathogens were not isolated throughout the study. Detection of WSSV in nested PCR is suggestive of a low viral load in shrimps. V. parahaemolyticus, an opportunistic pathogen that causes large scale mortalities in L. vannamei under unfavourable environmental was not detected. The study indicated low prevalence of bacterial and viral pathogens in shrimp culture environment suggesting that under favourable conditions could proliferate disease problems. The viral infection might predispose shrimps to infection by bacterial pathogens such as V. parahemolyticus and Vibrio harveyi. Diseases caused by bacterial and viral pathogens can be prevented by appropriate management practices, promotion of alternatives to antibiotics such as immune stimulants and vaccines.