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Subject: Agricultural Extension ×

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    ThesisItemOpen Access
    DETECTION AND MOLECULAR CHARACTERISATION OF CRYPTOSPORIDIUM SPECIES OF PIGS FROM BENGALURU REGION OF KARNATAKA
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR., 2022) MYREDDY NIKHIL; ANANDA K. J.
    The prevalence of GI parasites of pigs with special reference to molecular characterisation of Cryptosporidium species of pigs from Bengaluru region of Karnataka was carried out. A total of 336 pig faecal samples was examined, 115 (34.44 %) found positive for GI parasites. The piglets aged 0-3 months showed higher prevalence followed by 4-6 months and above 6 months. Breed wise prevalence revealed highest in indigenous followed by Duroc and Yorkshire. The gender wise showed higher in females (35.7 %) than in males (32.7 %). The diarrhoeic samples showed more GI parasites (47.45 %) than semisolid (46.09 %) and normal (20.98 %). Free range pigs harbour more GI parasites (47.32 %) than semi-intensive (35.71 %) and intensive (19.64 %). Hyostrongylus sp. was recorded as highest (14.2 %), followed by Ascaris sp. (13.6 %), Trichuris sp. (5.95 %), Physocephalus sp. (4.46 %), Balantidium cyst (4.46 %), Oesophagostomum sp. (3.27 %), Globochephalus sp. (2.97 %) and Metastrongylus spp. (0.59 %). The faecal sample examined by mZ-N showed 4.65 % positive for Cryptosporidium spp. and free-range pigs showed higher prevalence compare to semi-intensive and intensive. Sixty faecal DNA samples subjected to nested and conventional PCR targeting different genes of Cryptosporidium spp., showed 31.66 per cent positivity. The sequence analysis of PCR products and phylogenetic profile indicated the prevalence of C. scrofarum (pig genotype- II) and accession numbers OL69117, OL691173 and OL691174 were confirmed based on 100 per cent homology with Ludhiana isolate and 94.6 to 98.9 per cent with other existing isolates. Further, 60 DNA samples were subjected for nested and conventional PCR targeting different genes of Entamoeba sp., Giardia sp. and Cystoisospora sp. revealed ten positive for Entamoeba sp., one for Cystisospora sp. and three for Giardia sp. with a 141 prevalence rate of 16.6, 1.66 and 5.0 per cent respectively. The sequence analysis of PCR products indicated the presence of Entamoeba poleki and Cystoisospora suis in pigs. The species Cryptosporidium scrofarum (pig genotype-II), Entamoeba poleki and Cystoisospora suis were identified for the first time from southern Karnataka and are of zoonotic significance.