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Theses (PG)

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End date: 2009 × Start date: 2009 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    PHYTOPLANKTON DYNAMICS IN THE COASTAL WATERS OF PADUBIDRI, UDUPI DISTRICT
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2009-06) MEGHANA P.N.; H.R.V.REDDY; S.M.SHIVPRAKAS
    Seasonal variation of phytoplankton was studied in the coastal waters of Padubidri with reference to hydrographic parameters for a period of one year (February 2008 to January 2009). The sampling was made at monthly intervals at 5, 10, 15m depts. The salinity values ranged from 30.64 to 35.11ppt and exhibited bimodal seasonal oscillation with maximum values during pre monsoon period. The dissolved oxygen content varied between 4.0 and 6.5 mg/l with moderate values during pre monsoon period. The pH values were observed during post monsoon period. Among the nutrients ammonia and nitrite showed high values during post monsoon period while nitrate, phosphate and silicate showed higher values during pre monsoon period. Total phytoplankton population varied from 100 to 10, 95,000 cells/m3. Diatoms dominated the phytoplankton and contributed about 92% of total phytoplankton population followed by dinoflagellate (6%) and cyanobacteria (2%). In total 22 species of diatoms were encountered. The diatoms comprised of Bacteriastrum, Biddulphia, Cerataulina, Chaetoceros, Coscinodiscus, Cyclotella, Ditylum, Eucamphia, Leptocylindrus, Mellosira, Planktoniella, Rhizosolenia, Skeletonema, Streptotheca, Tricerratium, Asterionella, Fragillaria, Navicula, Nitzschia, Pleurosigma, Thalassionema, and Thallassiothrix..The dinophyceae was represented by 5 genera such as Ceratium, Dinophysis, Ornithoceros, Peridinium and Preperidinium and blue green algae by Tricodesmium and Oscillatoria The chlorophyll- “a” content fluctuated from 1.28 to 4.81mg/l Overall, phytoplankton population density was high during summer periods and low during post monsoon periods. The statistical analysis showed strong relationship between temperature, salinity, nutrients and phytoplankton abundance.
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    ThesisItemOpen Access
    EXPERIMENTAL STUDIES ON SILK PROTEIN MATERIAL FOR ITS EFFECT ON WOUND HEALING AND SAFETY IN LABORATORY ANIMALS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) SEVRINA RACHANA D'SOUZA
    Wound healing or wound repair is the process that follows injury to the skin and other soft tissues.
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    ThesisItemOpen Access
    PHARMACOLOGICAL AND SUBCHRONIC TOXICOLOGICAL STUDY OF Asteracantha longifolia IN RATS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) S. SHENBAGAM
    Asteracantha longifolia Nees. (Syn. Hygrophila spinosa, Hygrophila auriculata) is a common weed growing in marshy and water logged areas. It is an important medicinal herb, widely distributed in India and used by local population for different medicinal purposes
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    ThesisItemOpen Access
    TOXICITY STUDIES OF NORFLOXACIN, A FLUOROQUINOLONE IN RATS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) RASHMI, R
    Fluoroquinolones comprise a relatively large, growing and most interesting group of antibiotics
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    ThesisItemOpen Access
    TOXICITY STUDIES OF NORFLOXACIN IN BROILER CHICKENS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) MOHAMMAD BAYER DARMEL
    The term â chemotherapyâ ? was coined by Paul Ehrlich to describe the drugs which show aggression to invading organisms without damaging the host
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    ThesisItemOpen Access
    TOXICITY STUDIES OF RAW ARECA NUT EXTRACT IN RATS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) LOHITH, T.S
    Areca nut is a name given to the seed of the Areca catechu tree. Areca catechu is part of the Arecaceae family
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    ThesisItemOpen Access
    TOXICITY STUDIES OF ANNONA SQUAMOSA IN RATS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) DILIP,S.M
    Once the organisms have evolved in the universe, they adopt various mechanisms to survive in the environment
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    ThesisItemOpen Access
    Evaluation of Adjuvant Activity of Heat Shock Protein Fused to FMD Virus Polyprotein in Development of Mucosal Vaccine
    (Karnataka Veterinary, Animal and Fisheries Sciences University , Bidar, 2009-07-17) Usharani, J.; Shrikrishna, Isloor; Suryanarayana, V.V.S.; Rathnamma, D.; Veeregowda, B.M.; Reddy, G.R.; --
    The present work was undertaken to develop a mucosal vaccine as a therapeutic vaccine against FMD on the hypothesis that, the delivered candidate vaccine, if made anchored on mucosal surface may stimulate potent-IgA response, which can neutralize the virus during spread of the disease. In our present study, we made use of Enolase gene isolated from oral commensal of cattle, Enterococcus faecalis and FMDV polyvalent protein gene encoding major immunogenic epitopes of three serotypes (A22, Asia-1 and ‘O’). Enolase, a heat shock protein is identified to bind to plasminogen and to mucosal surface mainly by lysine residues in the C-terminal of the protein. α Enolase may be an excellent adapter molecule in the application of antigen delivery at the mucosal surface. The gene Enolase was used to produce a fusion protein with FMDV immunogen at the C-terminal end. For this, FMDV polyvalent protein gene was inserted at Not1 and Xho1 sites in the pET-32a+ vector. Enolase was inserted at Bam H1 and Xho1 sites of the pET-32a+ vector so as to get FMDV polyvalent protein gene at the 3’ end of Enolase. The construct was induced to get 3’fusion protein which was confirmed by immunoblot assay. The 3’fusion protein after purification was used for immune response study in guinea pigs. Oral wash and serum samples of these guinea pigs subjected to double sandwich ELISA showed that there was a specific IgA production.
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    ThesisItemOpen Access
    Immunological Evalution of Escherichia Coli Biofilm Vaccine in Cattle
    (Karnataka Veterinary, Animal and Fisheries Sciences University , Bidar, 2009-07-15) Chandrashekhara, N.; Shrikrishna, Isloor; Rathnamma, D.; yathiraj, S.; Sridhar, N.B.; Byregowda, S.M.; --, --
    The study was carried out to evaluate T cell proliferative response in cattle by analyzing lymphocyte subpopulation with reference to CD4 and CD8 cells by flow cytometry and humoral immune response with reference to serum IgG level by indirect ELISA, in mastitis causing E.coli Biofilm vaccinated and Free cell vaccinated groups. In all, eighteen cattle in early lactation which were free from mastitis were subjected to trials. Bentonite clay (an adjuvant by itself) based E.coli BF and FC vaccines were administered at 0, 30 and 60 days. Freund’s incomplete adjuvant was incorporated in the first shot. In Flow cytometric analysis, vaccination based on the E.coli BF showed significant enhancement in CD4 and CD8 cells. The percentage of CD4 and CD8 increased significantly in the E.coli BF vaccinated groups than in the control group and further, in comparison with E.coli FC vaccine, the percentage of CD4 and CD8 cell population found to be marginally increased on days 60 and 90 and significantly increased on day 120 post vaccination. Further, serum IgG level detected by indirect ELISA was significantly higher in BF vaccinated than FC vaccinated groups. The increased levels of IgG in the BF vaccinated serum coincided with the CD4 levels determined by the flow cytometry, indicating the higher titres of IgG due to the presence of CD4 cells. Both BF and FC vaccinated groups showed significant difference with control group. T cell proliferative immunological response by analyzing lymphocyte subpopulation with reference to CD4 and CD8 cells and humoral immune responses indicated superiority of bovine mastitis causing E.coli BF vaccine over FC vaccine.