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Author: Ayswarya R. Venu × End date: 2009 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Genetic Polymorphism of Major histocompatibility Complex Class Ii DRB Genes in Nandidurg Goats
    (Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, 15-06-13) Ayswarya R. Venu; Jayashankar, M.R.; Nagaraja, C.S.; Satyanarayan, K.; Byregowda, S.M.
    The current study was conducted to analyse the PCR-RFLP polymorphism and the sequences of second exon of Major histocompatibility complex class II DRB genein 82 Nandidurg goats. The amplified fragment with size of 285 bp was digested by the restriction enzyme Pst I. Two restriction patterns, A having fragments of size 226, 44 and15 bp, and B having fragements of size 270 and 15 bp were detected from Pst I RFLP. In case of heterozygotes, four bands of 270, 226, 44 and 15 bp fragments were resolved on the gel indicating the presence of two Pst I recognition site on one chromosome and one recognition site on the homologous pair. The gene frequencies of A and B were 0.51 and 0.49 respectively, and the genotype frequencies were 0.21 (AA), 0.59 (AB) and 0.20 (BB). The studied population of Nandidurg goats was found to be in Hardy Weinberg equilibrium. The sequencing of DRB gene exon two revealed two different DRB alleles which were more similar to Capra hircus (97-100%). The Pst I site was found to be associated with a TAC codon at position 78 and the absence was found to be associated with GTG codon in the sequenced sample. The close association of Pst I restriction sites and amino acid substitution at position 78 suggests that PCR-RFLP could be a useful tool in relating amino acid substitution at critical position with disease resistance.