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ThesisItem Open Access SEED GERMINATION TECHNIQUE {PUNYAKOTl TEST) TO DETECT EARLY PREGNANCY IN COWS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2006) S. V. KRISHNA RAOEarly pregnancy detection in dairy cows {Bos taiinis) is an integral part of a rational and successful animal husbandry practice. In this respect a plethora of pregnancy detection techniques have been tried in cows with variable degrees of reliability. These techniques broadly comprise physical (rectal palpation, radiography, laparoscopy and ultrasonography), chemical (cuboniĆ¢ s test,), biochemical (assay of hormones and pregnancy specific proteins by radioimmunoassay), immunological (rosette inhibition test for early pregnancy factor) (Wani et al., 2003) and biological methods (Ascheim-Zondek test).