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ThesisItem Open Access COMPARATIVE STUDY ON OVSYNCH AND PROGESTERONE SPONGE BASED ESTRUS SYNCHRONIZATION PROTOCOLS IN MANDYA SHEEP(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2023) PRATHVIRAJ; SRINIVAS REDDY BELLURThe present study based on two estrus synchronization protocols in 30 multiparous Mandya breed of sheep. Study was carried out at the Livestock Research and Information Centre Nagamangala, KVAFSU, Bidar, during the breeding season. The ewes divided into two groups (15 each). OVSYNCH was followed for protocol 1, and Progesterone + OVSYNCH was followed for protocol 2. Blood samples from study animals were collected at day, 0, day 7, day of estrus/mating, 30 days post mating and at the time of mating. Pregnancy diagnosis were done 30 days post mating using ultrasonography The reproductive parameters such as estrus response, time for onset of estrus, rate of conception, lambing percentages, twinning rate, lamb birth weight and lambs sex ratio were recorded. Serum estrogen and progesterone were analyzed using commercially available ELISA kits, and serum biochemical parameters glucose, total protein, albumin, cholesterol, triglycerides and blood urea nitrogen were analyzed using reagent kits. The estrus response was 60.00 % for protocol 1 and 80.00 % for protocol 2. The average interval for onset of estrus were 19.00 hours for protocol 1 and 24.75 hours for protocol 2. The rate of conception during present study were 40.00 % in protocol 1 and 60.00 % in protocol 2. The lambing percentage on total ewe basis were 40 % and 60 %, lambing percentage on estrus basis were 66.60 % and 75.00 %, lambing percentage on tupping basis were 75.00 % and 90.00 % in protocol 1 and 2 respectively. The average Lamb birth weights were 1.97 kg and 2.06 Kg, Male: Female sex ratio was 1:1 and 1:1.25 for protocol 1 and 2 respectively. Significantly (P˂0.05) higher serum estrogen levels were noted at day of estrus/mating and on the day of lambing in both protocol 1 and protocol 2. Significantly lowest serum progesterone levels were observed at day of estrus/mating, in both the protocol 1 and protocol 2. Significantly (P˂ 0.05) lowest serum glucose levels were observed at day of lambing compared to all other stages of study period in both the protocols. Significantly lowest serum total protein and albumin levels were observed at day of lambing compared to all other stages of study period in both the protocols. Significantly lowest serum cholesterol levels were observed at day 0 and day 7 in both protocols. Significantly lowest levels were observed at day 0 and day 7 in protocol 1 and at day 0 in protocol 2 compared to all other stages of study period. Key words: Mandya ewe, Estrus synchronization, Reproductive parameter, Serum biochemistry
