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20114
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Subject: Veterinary Microbiology Ɨ End date: 2011 Ɨ Start date: 2011 Ɨ Type: Thesis Ɨ
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    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF MAREKĆ¢ S DISEASE VIRUS FROM CHICKEN
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2011) ROOPA KADASANI
    Poultry keeping in India is almost 5,000 years old, however it witnessed a remarkablegrowth from backyard to commercial industry the in the last four decades
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    ThesisItemOpen Access
    ISOLATION AND MOLECULAR CHARACTERIZATION OF INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2011) RANGANATHA, S
    India is the largest milk producer in the world with an annual production of 104 million tones in 2008 whereas world milk production reached at 684 million tons in 2008.
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    DETERMINATION OF VIRULENCE FACTORS OF MAJOR STREPTOCOCCI ISOLATED FROM BOVINE MASTITIS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2011) KRISHNAVENI, N
    India is the largest milk producer of the world producing more than 100 million tons of milk per annum accounting to about 13 per cent of globalproduction and 57 per cent of total AsiaĆ¢ s production with 185 million cattle and 98 million buffaloes (Livestock Census, 2007).
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    ThesisItemOpen Access
    Isolation and Molecular Characterization of Infectious Bovine Rhinotracheitis Virus.
    (Karnataka Veterinary, Animal and Fisheries Sciences University , Bidar, 2011-08-16) Ranganatha, S.; Rathnamma, D.; Shrikrishna, Isloor; Veeregowda, B.M.; Narayana Bhat, M.; Patil, S.S.; --, --
    Infectious Bovine Rhinotracheitis is one of the important viral disease of cattle caused by Bovine herpes Virus -1. In India, IBR causes great economic loss to the farmers and its prevalence is in increasing trend over the past 10 years. The present study was undertaken to isolate and characterise the BoHV-1 isolates circulating in Karnataka state. Samples such as Nasal swabs, conjunctival swabs and aborted foetal liver were collected from 40 cattle showing symptoms similar to IBR. All the samples were subjected for virus isolation in MDBK cell line. Among the 40 samples only three samples yielded BoHV-1 isolates in cell culture which were confirmed by Virus neutralisation test. A sensitive and specific PCR was developed targeting conserved region of gC gene and applied directly to the DNA extracted from field samples. The sensitivity of the designed gC specific primers was 75 per cent and compared with the published primers targeting gB gene whose sensitivity was 66 per cent. The gC based PCR products were subjected for restriction enzyme analysis which further confirmed the isolates as BoHV-1. The amplified product of gC based PCR was cloned in pGEMT vector and sequenced. The nucleotide sequences of gC gene of BoHV-1 field isolates viz., PD_ADMAS707/10, PD_ADMAS723/10, PD_ADMAS741/10 along with the reference virus were compared with published sequences using the BLAST programme and submitted to GenBank under the accession numbers JN022593, JN022594, JN171865 respectively. Phylogenetic analysis revealed that all three field isolates were clustered with BoHV-1.1 subtype. Thus, the study revealed the persistence of BoHV-1.1 subtype in India.