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Theses (PG)

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2008 - 20093
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Subject: Veterinary Microbiology × End date: 2009 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    ISOLATION OF BACTERIOPHAGE AND ITS EVALUATION OF THERAPEUTIC EFFICACY ON EXPERIMENTALLY INDUCED ESCHERICHIA COU INFECTION IN CHICKENS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2008) CHArrHRA.B.S
    An ever changing path of innovation and modernization has lead to the metamorphosis of primitive poultry farming system into an industry which is highly profitable, contributing substantially to national exchequer and making India one of the top nations in the field of poultry production.
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    ThesisItemOpen Access
    Evaluation of Adjuvant Activity of Heat Shock Protein Fused to FMD Virus Polyprotein in Development of Mucosal Vaccine
    (Karnataka Veterinary, Animal and Fisheries Sciences University , Bidar, 2009-07-17) Usharani, J.; Shrikrishna, Isloor; Suryanarayana, V.V.S.; Rathnamma, D.; Veeregowda, B.M.; Reddy, G.R.; --
    The present work was undertaken to develop a mucosal vaccine as a therapeutic vaccine against FMD on the hypothesis that, the delivered candidate vaccine, if made anchored on mucosal surface may stimulate potent-IgA response, which can neutralize the virus during spread of the disease. In our present study, we made use of Enolase gene isolated from oral commensal of cattle, Enterococcus faecalis and FMDV polyvalent protein gene encoding major immunogenic epitopes of three serotypes (A22, Asia-1 and ‘O’). Enolase, a heat shock protein is identified to bind to plasminogen and to mucosal surface mainly by lysine residues in the C-terminal of the protein. α Enolase may be an excellent adapter molecule in the application of antigen delivery at the mucosal surface. The gene Enolase was used to produce a fusion protein with FMDV immunogen at the C-terminal end. For this, FMDV polyvalent protein gene was inserted at Not1 and Xho1 sites in the pET-32a+ vector. Enolase was inserted at Bam H1 and Xho1 sites of the pET-32a+ vector so as to get FMDV polyvalent protein gene at the 3’ end of Enolase. The construct was induced to get 3’fusion protein which was confirmed by immunoblot assay. The 3’fusion protein after purification was used for immune response study in guinea pigs. Oral wash and serum samples of these guinea pigs subjected to double sandwich ELISA showed that there was a specific IgA production.
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    ThesisItemOpen Access
    Immunological Evalution of Escherichia Coli Biofilm Vaccine in Cattle
    (Karnataka Veterinary, Animal and Fisheries Sciences University , Bidar, 2009-07-15) Chandrashekhara, N.; Shrikrishna, Isloor; Rathnamma, D.; yathiraj, S.; Sridhar, N.B.; Byregowda, S.M.; --, --
    The study was carried out to evaluate T cell proliferative response in cattle by analyzing lymphocyte subpopulation with reference to CD4 and CD8 cells by flow cytometry and humoral immune response with reference to serum IgG level by indirect ELISA, in mastitis causing E.coli Biofilm vaccinated and Free cell vaccinated groups. In all, eighteen cattle in early lactation which were free from mastitis were subjected to trials. Bentonite clay (an adjuvant by itself) based E.coli BF and FC vaccines were administered at 0, 30 and 60 days. Freund’s incomplete adjuvant was incorporated in the first shot. In Flow cytometric analysis, vaccination based on the E.coli BF showed significant enhancement in CD4 and CD8 cells. The percentage of CD4 and CD8 increased significantly in the E.coli BF vaccinated groups than in the control group and further, in comparison with E.coli FC vaccine, the percentage of CD4 and CD8 cell population found to be marginally increased on days 60 and 90 and significantly increased on day 120 post vaccination. Further, serum IgG level detected by indirect ELISA was significantly higher in BF vaccinated than FC vaccinated groups. The increased levels of IgG in the BF vaccinated serum coincided with the CD4 levels determined by the flow cytometry, indicating the higher titres of IgG due to the presence of CD4 cells. Both BF and FC vaccinated groups showed significant difference with control group. T cell proliferative immunological response by analyzing lymphocyte subpopulation with reference to CD4 and CD8 cells and humoral immune responses indicated superiority of bovine mastitis causing E.coli BF vaccine over FC vaccine.