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ThesisItem Open Access COMPARATIVE EVALUATION OE RT-PCR EOR VP3, VP7 AND NS3 GENES IN DETECTING BLUETONGUE VIRUS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) VINAY, SThe Indian farming community mostly comprises of farmers of poor section having sheep and goat as their integral part contributing significantly to the nationâ s economyThesisItem Open Access STANDARDIZATION OF INDIRECT ELISA FOR SEROPREVALENCE OF LEPTOSPIROSIS IN BOVINES(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) SENTHILNATHAN, GLeptospirosis is a widespread zoonosis caused by Leptospira interrogans, a pathogen capable of infecting most mammalian species through either direct contact with an infected animal or indirect contact with contaminated soil or water.ThesisItem Open Access STUDY ON THE IMMUNOGENIC EFFECT OF FIMBRIAL ANTIGEN EXTRACTED FROM E. co//SEROTYPES OF POULTRY ORIGIN(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) PRADNYANKUR L BORKARThe world poultry industry has grown to a mega industry and the broiler chicken industry has attained the second place in the world after pork. The chicken meat represents 29% of the total meat from farm animals. The enhanced growth in layer and broiler industry has become possible due to the strong consumer demand for product that are cheap, safe and healthy, 'fhere has been a rapid growth in the poultry industry over the last four decades due to the transformation from backyard stage to an organized dynamic agro-industry with growlh rates of 4-6% in layers and 8 10% in broilers, respectively.ThesisItem Open Access STANDARDIZATION OF MILK BASED ELISA IN RABBITS IMMUNIZED WITH BOVINE MASTITIS CAUSING Escherichia coli AND Staphylococcus aureus BIOFILM VACCINES(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) JYOTHI, H.LBovine mastitis is one of the most problematic diseases and continues to have major economic impact on the dairy industry throughout the world. It is difficult to estimate the losses associated with clinical mastitis, which arise from the cost of treatment and culling of animals with decreased milk production (Bradley, 2002). However, it is estimated that mastitis alone causes approximately 70 per cent of all avoidable losses incurred during milk production. A world wide annual loss due to mastitis is estimated to be $ 35 billions. The total economic losses due to mastitis alone accounts up to Rs. 6053.21 crores per annum in India (Dua, 2001).ThesisItem Open Access EVALUATION OF ADJUVANT ACTIVITY OF HEAT SHOCK PROTEIN FUSED TO FMD VIRUS POLYPROTEIN IN DEVELOPMENT OF MUCOSAL VACCINE(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) USHARANI, JThe global livestock industry is facing a constant threat from Foot and Mouth Disease (FMD), the highly contagious and most economically important diseaseThesisItem Open Access IMMUNOLOGICAL EVALUTION OF Escherichia coli BIOFILM VACCINE IN CATTLE(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) CHANDRASHEKHARA, NThe Indian dairy industry has shown remarkable development in the past decade and is rapidly growingThesisItem Open Access IMMUNOLOGICAL EVALUTION OF Escherichia coli BIOFILM VACCINE IN CATTLE(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) CHANDRASHEKHARA, NThe Indian dairy industry has shown remarkable development in the past decade and is rapidly growing.ThesisItem Open Access AMPLIFICATION AND CLONING OF p29 GENE OF Mycoplasma gallisepticum(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) VINAYKUMAR, Y. LIndia's poultry industry has transformed from a mere backyard activity into a major commercial activity in just four decades. Today, it is one of the fastest growing segments of the agricultural sector in India. While the production of agricultural crops has been rising at a rate of 1.5 to 2 per cent per annum, but that of eggs and broilers has been rising at a rate of 8 to 10 per cent per annum. India ranks third in egg production and sixth in chicken meat production (www.ildexindia.com, 2008).ThesisItem Open Access Evaluation of Adjuvant Activity of Heat Shock Protein Fused to FMD Virus Polyprotein in Development of Mucosal Vaccine(Karnataka Veterinary, Animal and Fisheries Sciences University , Bidar, 2009-07-17) Usharani, J.; Shrikrishna, Isloor; Suryanarayana, V.V.S.; Rathnamma, D.; Veeregowda, B.M.; Reddy, G.R.; --The present work was undertaken to develop a mucosal vaccine as a therapeutic vaccine against FMD on the hypothesis that, the delivered candidate vaccine, if made anchored on mucosal surface may stimulate potent-IgA response, which can neutralize the virus during spread of the disease. In our present study, we made use of Enolase gene isolated from oral commensal of cattle, Enterococcus faecalis and FMDV polyvalent protein gene encoding major immunogenic epitopes of three serotypes (A22, Asia-1 and ‘O’). Enolase, a heat shock protein is identified to bind to plasminogen and to mucosal surface mainly by lysine residues in the C-terminal of the protein. α Enolase may be an excellent adapter molecule in the application of antigen delivery at the mucosal surface. The gene Enolase was used to produce a fusion protein with FMDV immunogen at the C-terminal end. For this, FMDV polyvalent protein gene was inserted at Not1 and Xho1 sites in the pET-32a+ vector. Enolase was inserted at Bam H1 and Xho1 sites of the pET-32a+ vector so as to get FMDV polyvalent protein gene at the 3’ end of Enolase. The construct was induced to get 3’fusion protein which was confirmed by immunoblot assay. The 3’fusion protein after purification was used for immune response study in guinea pigs. Oral wash and serum samples of these guinea pigs subjected to double sandwich ELISA showed that there was a specific IgA production.
